Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2

Fly Dicer-2 is thought to use two distinct – processive or distributive – modes of cleavage by distinguishing the terminal structures of double-stranded RNA (dsRNA) substrates with the help of its cofactor LoquaciousPD (Loqs-PD). Here the authors show by single-molecule imaging that dsRNA terminal s...

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Autores principales:	Masahiro Naganuma, Hisashi Tadakuma, Yukihide Tomari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/075f930718904d9eb6b1018d79517ab4
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spelling	oai:doaj.org-article:075f930718904d9eb6b1018d79517ab42021-12-02T16:08:13ZSingle-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-210.1038/s41467-021-24555-12041-1723https://doaj.org/article/075f930718904d9eb6b1018d79517ab42021-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-24555-1https://doaj.org/toc/2041-1723Fly Dicer-2 is thought to use two distinct – processive or distributive – modes of cleavage by distinguishing the terminal structures of double-stranded RNA (dsRNA) substrates with the help of its cofactor LoquaciousPD (Loqs-PD). Here the authors show by single-molecule imaging that dsRNA terminal structures and Loqs-PD change the probability for Dicer to initiate processive cleavage but not the mode of cleavage action per se.Masahiro NaganumaHisashi TadakumaYukihide TomariNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Masahiro Naganuma Hisashi Tadakuma Yukihide Tomari Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
description	Fly Dicer-2 is thought to use two distinct – processive or distributive – modes of cleavage by distinguishing the terminal structures of double-stranded RNA (dsRNA) substrates with the help of its cofactor LoquaciousPD (Loqs-PD). Here the authors show by single-molecule imaging that dsRNA terminal structures and Loqs-PD change the probability for Dicer to initiate processive cleavage but not the mode of cleavage action per se.
format	article
author	Masahiro Naganuma Hisashi Tadakuma Yukihide Tomari
author_facet	Masahiro Naganuma Hisashi Tadakuma Yukihide Tomari
author_sort	Masahiro Naganuma
title	Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
title_short	Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
title_full	Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
title_fullStr	Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
title_full_unstemmed	Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2
title_sort	single-molecule analysis of processive double-stranded rna cleavage by drosophila dicer-2
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/075f930718904d9eb6b1018d79517ab4
work_keys_str_mv	AT masahironaganuma singlemoleculeanalysisofprocessivedoublestrandedrnacleavagebydrosophiladicer2 AT hisashitadakuma singlemoleculeanalysisofprocessivedoublestrandedrnacleavagebydrosophiladicer2 AT yukihidetomari singlemoleculeanalysisofprocessivedoublestrandedrnacleavagebydrosophiladicer2
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Single-molecule analysis of processive double-stranded RNA cleavage by Drosophila Dicer-2

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