Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells

Abstract All over the world, different types of nanomaterials with a diversified spectrum of applications are designed and developed, especially in the field of nanomedicine. The great variety of nanoparticles (NPs), in vitro test systems and cell lines led to a vast amount of publications with conf...

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Autores principales:	Lisa Landgraf, Daniel Nordmeyer, Peter Schmiel, Qi Gao, Sandra Ritz, Julia S. Gebauer, Stefan Graß, Silvia Diabaté, Lennart Treuel, Christina Graf, Eckart Rühl, Katharina Landfester, Volker Mailänder, Carsten Weiss, Reinhard Zellner, Ingrid Hilger
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0767725af98b41829a2aceae8fe4321c
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spelling	oai:doaj.org-article:0767725af98b41829a2aceae8fe4321c2021-12-02T12:32:36ZValidation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells10.1038/s41598-017-02958-92045-2322https://doaj.org/article/0767725af98b41829a2aceae8fe4321c2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02958-9https://doaj.org/toc/2045-2322Abstract All over the world, different types of nanomaterials with a diversified spectrum of applications are designed and developed, especially in the field of nanomedicine. The great variety of nanoparticles (NPs), in vitro test systems and cell lines led to a vast amount of publications with conflicting data. To identify the decisive principles of these variabilities, we conducted an intercomparison study of collaborating laboratories within the German DFG Priority Program SPP1313, using well-defined experimental parameters and well-characterized NPs. The participants analyzed the in vitro biocompatibility of silica and polymer NPs on human hepatoma HepG2 cells. Nanoparticle mediated effects on cell metabolism, internalization, and inflammation were measured. All laboratories showed that both nanoparticle formulations were internalized and had a low cytotoxicity profile. Interestingly, small variations in nanoparticle preparation, cell handling and the type of culture slide influenced the nanoparticle stability and the outcomes of cell assays. The round robin test demonstrated the importance of the use of clearly defined and characterized NPs and parameters for reproducible results across laboratories. Comparative analyses of in vitro screening methods performed in multiple laboratories are absolutely essential to establish robust standard operation procedure as a prerequisite for sound hazard assessment of nanomaterials.Lisa LandgrafDaniel NordmeyerPeter SchmielQi GaoSandra RitzJulia S. GebauerStefan GraßSilvia DiabatéLennart TreuelChristina GrafEckart RühlKatharina LandfesterVolker MailänderCarsten WeissReinhard ZellnerIngrid HilgerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lisa Landgraf Daniel Nordmeyer Peter Schmiel Qi Gao Sandra Ritz Julia S. Gebauer Stefan Graß Silvia Diabaté Lennart Treuel Christina Graf Eckart Rühl Katharina Landfester Volker Mailänder Carsten Weiss Reinhard Zellner Ingrid Hilger Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells
description	Abstract All over the world, different types of nanomaterials with a diversified spectrum of applications are designed and developed, especially in the field of nanomedicine. The great variety of nanoparticles (NPs), in vitro test systems and cell lines led to a vast amount of publications with conflicting data. To identify the decisive principles of these variabilities, we conducted an intercomparison study of collaborating laboratories within the German DFG Priority Program SPP1313, using well-defined experimental parameters and well-characterized NPs. The participants analyzed the in vitro biocompatibility of silica and polymer NPs on human hepatoma HepG2 cells. Nanoparticle mediated effects on cell metabolism, internalization, and inflammation were measured. All laboratories showed that both nanoparticle formulations were internalized and had a low cytotoxicity profile. Interestingly, small variations in nanoparticle preparation, cell handling and the type of culture slide influenced the nanoparticle stability and the outcomes of cell assays. The round robin test demonstrated the importance of the use of clearly defined and characterized NPs and parameters for reproducible results across laboratories. Comparative analyses of in vitro screening methods performed in multiple laboratories are absolutely essential to establish robust standard operation procedure as a prerequisite for sound hazard assessment of nanomaterials.
format	article
author	Lisa Landgraf Daniel Nordmeyer Peter Schmiel Qi Gao Sandra Ritz Julia S. Gebauer Stefan Graß Silvia Diabaté Lennart Treuel Christina Graf Eckart Rühl Katharina Landfester Volker Mailänder Carsten Weiss Reinhard Zellner Ingrid Hilger
author_facet	Lisa Landgraf Daniel Nordmeyer Peter Schmiel Qi Gao Sandra Ritz Julia S. Gebauer Stefan Graß Silvia Diabaté Lennart Treuel Christina Graf Eckart Rühl Katharina Landfester Volker Mailänder Carsten Weiss Reinhard Zellner Ingrid Hilger
author_sort	Lisa Landgraf
title	Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells
title_short	Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells
title_full	Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells
title_fullStr	Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells
title_full_unstemmed	Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells
title_sort	validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of sio2 and polymer nanoparticles in hepg2 cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/0767725af98b41829a2aceae8fe4321c
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_version_	1718394018299117568

Validation of weak biological effects by round robin experiments: cytotoxicity/biocompatibility of SiO2 and polymer nanoparticles in HepG2 cells

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