INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS

Abstract. Alpha-1-acid glycoprotein (orosomucoid) is a multifunctional acute phase reactant belonging to the family of lipocalines from plasma alpha-2 globulin fraction. In present study, we investigated dosedependent effects of orosomucoid upon secretion of IL-1â, IL-2, IL-3, IL-4 by mononuclear ce...

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Autores principales:	М. V. Osikov, L. V. Krivohizhina, E. V. Makarov, V. Yu. Akhmatov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	alpha-1-acid glycoprotein cytokines mononuclear cells Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/076d9d2d5ff24e9abcbf5b476fd71b20
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spelling	oai:doaj.org-article:076d9d2d5ff24e9abcbf5b476fd71b202021-11-18T08:03:38ZINFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS1563-06252313-741X10.15789/1563-0625-2007-1-47-52https://doaj.org/article/076d9d2d5ff24e9abcbf5b476fd71b202014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/119https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Alpha-1-acid glycoprotein (orosomucoid) is a multifunctional acute phase reactant belonging to the family of lipocalines from plasma alpha-2 globulin fraction. In present study, we investigated dosedependent effects of orosomucoid upon secretion of IL-1â, IL-2, IL-3, IL-4 by mononuclear cells from venous blood of healthy volunteers. Mononuclear cells were separated by means of gradient centrifugation, followed by incubation for 24 hours with 250, 500, or 1000 mcg of orosomucoid per ml RPMI-1640 medium (resp., low, medium and high dose). The levels of cytokine production were assayed by ELISA technique. Orosomucoid-induced secretion of IL-1â and IL-4 was increased, whereas IL-3 secretion was inhibited. IL-2 production was suppressed at low doses of orosomucoid, and stimulated at medium and high doses. The effect of alpha-1-acid glycoprotein upon production of IL-2, IL-3 and IL-4 was dose-dependent. Hence, these data indicate that orosomucoid is capable of modifying IL-1â, IL-2, IL-3, and IL-4 secretion by blood mononuclear cells.М. V. OsikovL. V. KrivohizhinaE. V. MakarovV. Yu. AkhmatovSPb RAACIarticlealpha-1-acid glycoproteincytokinesmononuclear cellsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 9, Iss 1, Pp 47-52 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	alpha-1-acid glycoprotein cytokines mononuclear cells Immunologic diseases. Allergy RC581-607
spellingShingle	alpha-1-acid glycoprotein cytokines mononuclear cells Immunologic diseases. Allergy RC581-607 М. V. Osikov L. V. Krivohizhina E. V. Makarov V. Yu. Akhmatov INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS
description	Abstract. Alpha-1-acid glycoprotein (orosomucoid) is a multifunctional acute phase reactant belonging to the family of lipocalines from plasma alpha-2 globulin fraction. In present study, we investigated dosedependent effects of orosomucoid upon secretion of IL-1â, IL-2, IL-3, IL-4 by mononuclear cells from venous blood of healthy volunteers. Mononuclear cells were separated by means of gradient centrifugation, followed by incubation for 24 hours with 250, 500, or 1000 mcg of orosomucoid per ml RPMI-1640 medium (resp., low, medium and high dose). The levels of cytokine production were assayed by ELISA technique. Orosomucoid-induced secretion of IL-1â and IL-4 was increased, whereas IL-3 secretion was inhibited. IL-2 production was suppressed at low doses of orosomucoid, and stimulated at medium and high doses. The effect of alpha-1-acid glycoprotein upon production of IL-2, IL-3 and IL-4 was dose-dependent. Hence, these data indicate that orosomucoid is capable of modifying IL-1â, IL-2, IL-3, and IL-4 secretion by blood mononuclear cells.
format	article
author	М. V. Osikov L. V. Krivohizhina E. V. Makarov V. Yu. Akhmatov
author_facet	М. V. Osikov L. V. Krivohizhina E. V. Makarov V. Yu. Akhmatov
author_sort	М. V. Osikov
title	INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS
title_short	INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS
title_full	INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS
title_fullStr	INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS
title_full_unstemmed	INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS
title_sort	influence of alpha-1-acid glycoprotein upon production of cytokines by peripheral blood mononuclears
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/076d9d2d5ff24e9abcbf5b476fd71b20
work_keys_str_mv	AT mvosikov influenceofalpha1acidglycoproteinuponproductionofcytokinesbyperipheralbloodmononuclears AT lvkrivohizhina influenceofalpha1acidglycoproteinuponproductionofcytokinesbyperipheralbloodmononuclears AT evmakarov influenceofalpha1acidglycoproteinuponproductionofcytokinesbyperipheralbloodmononuclears AT vyuakhmatov influenceofalpha1acidglycoproteinuponproductionofcytokinesbyperipheralbloodmononuclears
_version_	1718422522310950912

INFLUENCE OF ALPHA-1-ACID GLYCOPROTEIN UPON PRODUCTION OF CYTOKINES BY PERIPHERAL BLOOD MONONUCLEARS

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