An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells

An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells

Abstract GADD45A (growth arrest and DNA damage inducible alpha), a stress response gene induced by genotoxic and nongenotoxic stresses, is implicated in various key processes, including the control of cell cycle checkpoints and DNA repair. The expression of GADD45A is directly regulated by numerous...

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Autores principales: Jie Li, Jie Dong, Shaohua Li, Wei Xia, Xueting Su, Xingliang Qin, Ying Chen, Hongmei Ding, Hui Li, Aixue Huang, Chenjun Bai, Tongnan Hu, Chenglong Wang, Bingfeng Chu, Ningsheng Shao
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Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/0777e147f5584144a906a00fb7c35159
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spelling oai:doaj.org-article:0777e147f5584144a906a00fb7c351592021-12-02T11:52:27ZAn alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells10.1038/s41598-017-07332-32045-2322https://doaj.org/article/0777e147f5584144a906a00fb7c351592017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07332-3https://doaj.org/toc/2045-2322Abstract GADD45A (growth arrest and DNA damage inducible alpha), a stress response gene induced by genotoxic and nongenotoxic stresses, is implicated in various key processes, including the control of cell cycle checkpoints and DNA repair. The expression of GADD45A is directly regulated by numerous transcription factors, with p53 being the most representative. Moreover, post-transcriptional regulation also plays a role in GADD45A expression. However, little is known about the regulatory effects of microRNAs (miRNAs) on GADD45A expression. As a potential tumour suppressor, miR-138 has pleiotropic biological functions in various cancers. We have previously reported p53-mediated activation of miR-138 in human non-small-cell lung cancer (NSCLC) cells. In this study, we found that miR-138 specifically targeted AGO2, which affects the stability and maturation of miR-130b. Decreased expression of miR-130b promoted the expression of GADD45A and resulted in the G2/M phase arrest and proliferation inhibition in human NSCLC cells. Our results suggested that p53 could alternatively upregulate GADD45A in human NSCLC cells through a post-transcriptional pathway in which miR-138 is involved.Jie LiJie DongShaohua LiWei XiaXueting SuXingliang QinYing ChenHongmei DingHui LiAixue HuangChenjun BaiTongnan HuChenglong WangBingfeng ChuNingsheng ShaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Jie Li
Jie Dong
Shaohua Li
Wei Xia
Xueting Su
Xingliang Qin
Ying Chen
Hongmei Ding
Hui Li
Aixue Huang
Chenjun Bai
Tongnan Hu
Chenglong Wang
Bingfeng Chu
Ningsheng Shao
An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
description Abstract GADD45A (growth arrest and DNA damage inducible alpha), a stress response gene induced by genotoxic and nongenotoxic stresses, is implicated in various key processes, including the control of cell cycle checkpoints and DNA repair. The expression of GADD45A is directly regulated by numerous transcription factors, with p53 being the most representative. Moreover, post-transcriptional regulation also plays a role in GADD45A expression. However, little is known about the regulatory effects of microRNAs (miRNAs) on GADD45A expression. As a potential tumour suppressor, miR-138 has pleiotropic biological functions in various cancers. We have previously reported p53-mediated activation of miR-138 in human non-small-cell lung cancer (NSCLC) cells. In this study, we found that miR-138 specifically targeted AGO2, which affects the stability and maturation of miR-130b. Decreased expression of miR-130b promoted the expression of GADD45A and resulted in the G2/M phase arrest and proliferation inhibition in human NSCLC cells. Our results suggested that p53 could alternatively upregulate GADD45A in human NSCLC cells through a post-transcriptional pathway in which miR-138 is involved.
format article
author Jie Li
Jie Dong
Shaohua Li
Wei Xia
Xueting Su
Xingliang Qin
Ying Chen
Hongmei Ding
Hui Li
Aixue Huang
Chenjun Bai
Tongnan Hu
Chenglong Wang
Bingfeng Chu
Ningsheng Shao
author_facet Jie Li
Jie Dong
Shaohua Li
Wei Xia
Xueting Su
Xingliang Qin
Ying Chen
Hongmei Ding
Hui Li
Aixue Huang
Chenjun Bai
Tongnan Hu
Chenglong Wang
Bingfeng Chu
Ningsheng Shao
author_sort Jie Li
title An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
title_short An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
title_full An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
title_fullStr An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
title_full_unstemmed An alternative microRNA-mediated post-transcriptional regulation of GADD45A by p53 in human non-small-cell lung cancer cells
title_sort alternative microrna-mediated post-transcriptional regulation of gadd45a by p53 in human non-small-cell lung cancer cells
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/0777e147f5584144a906a00fb7c35159
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