The influence of different sample preparation on mechanical properties of human iliotibial tract

Abstract In the run-up to biomechanical testing, fresh human tissue samples are often frozen in order to inhibit initial decomposition processes and to achieve a temporal independence of tissue acquisition from biomechanical testing. The aim of this study was to compare the mechanical properties of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Benjamin Fischer, Sascha Kurz, Andreas Höch, Stefan Schleifenbaum
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0778fa5b51f84d41b4d38aed1169d302
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0778fa5b51f84d41b4d38aed1169d302
record_format	dspace
spelling	oai:doaj.org-article:0778fa5b51f84d41b4d38aed1169d3022021-12-02T17:41:17ZThe influence of different sample preparation on mechanical properties of human iliotibial tract10.1038/s41598-020-71790-52045-2322https://doaj.org/article/0778fa5b51f84d41b4d38aed1169d3022020-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-71790-5https://doaj.org/toc/2045-2322Abstract In the run-up to biomechanical testing, fresh human tissue samples are often frozen in order to inhibit initial decomposition processes and to achieve a temporal independence of tissue acquisition from biomechanical testing. The aim of this study was to compare the mechanical properties of fresh tissue samples of the human iliotibial tract (IT) to fresh-frozen samples taken from the same IT and those modified with different concentrations of Dimethylsulfoxide (DMSO) prior to freezing. All samples were partial plastinated and destructive tensile tests were conducted with a uniaxial tensile test setup. A plastination technique already established in the laboratory was modified to improve the clamping behaviour of the samples. Material failure was caused by a gradual rupture of the load-bearing collagen fibre bundles. Contrary to our expectations, no significant difference was found between the tensile strength of fresh and fresh frozen specimens. The addition of 1 wt% DMSO did not increase the tensile strength compared to fresh-frozen samples; an addition of 10 wt% DMSO even resulted in a decrease. Based on our findings, the use of simple fresh-frozen specimens to determine the tensile strength is viable; however fresh specimens should be used to generate a complete property profile.Benjamin FischerSascha KurzAndreas HöchStefan SchleifenbaumNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Benjamin Fischer Sascha Kurz Andreas Höch Stefan Schleifenbaum The influence of different sample preparation on mechanical properties of human iliotibial tract
description	Abstract In the run-up to biomechanical testing, fresh human tissue samples are often frozen in order to inhibit initial decomposition processes and to achieve a temporal independence of tissue acquisition from biomechanical testing. The aim of this study was to compare the mechanical properties of fresh tissue samples of the human iliotibial tract (IT) to fresh-frozen samples taken from the same IT and those modified with different concentrations of Dimethylsulfoxide (DMSO) prior to freezing. All samples were partial plastinated and destructive tensile tests were conducted with a uniaxial tensile test setup. A plastination technique already established in the laboratory was modified to improve the clamping behaviour of the samples. Material failure was caused by a gradual rupture of the load-bearing collagen fibre bundles. Contrary to our expectations, no significant difference was found between the tensile strength of fresh and fresh frozen specimens. The addition of 1 wt% DMSO did not increase the tensile strength compared to fresh-frozen samples; an addition of 10 wt% DMSO even resulted in a decrease. Based on our findings, the use of simple fresh-frozen specimens to determine the tensile strength is viable; however fresh specimens should be used to generate a complete property profile.
format	article
author	Benjamin Fischer Sascha Kurz Andreas Höch Stefan Schleifenbaum
author_facet	Benjamin Fischer Sascha Kurz Andreas Höch Stefan Schleifenbaum
author_sort	Benjamin Fischer
title	The influence of different sample preparation on mechanical properties of human iliotibial tract
title_short	The influence of different sample preparation on mechanical properties of human iliotibial tract
title_full	The influence of different sample preparation on mechanical properties of human iliotibial tract
title_fullStr	The influence of different sample preparation on mechanical properties of human iliotibial tract
title_full_unstemmed	The influence of different sample preparation on mechanical properties of human iliotibial tract
title_sort	influence of different sample preparation on mechanical properties of human iliotibial tract
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/0778fa5b51f84d41b4d38aed1169d302
work_keys_str_mv	AT benjaminfischer theinfluenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract AT saschakurz theinfluenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract AT andreashoch theinfluenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract AT stefanschleifenbaum theinfluenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract AT benjaminfischer influenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract AT saschakurz influenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract AT andreashoch influenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract AT stefanschleifenbaum influenceofdifferentsamplepreparationonmechanicalpropertiesofhumaniliotibialtract
_version_	1718379686596182016

The influence of different sample preparation on mechanical properties of human iliotibial tract

Ejemplares similares