Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.

The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and cl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Karel Bezstarosti, Mart M Lamers, Wouter A S Doff, Peter C Wever, Khoa T D Thai, Jeroen J A van Kampen, Bart L Haagmans, Jeroen A A Demmers
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/077cab94180b48079fe6484c8ab0ec50
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:077cab94180b48079fe6484c8ab0ec50
record_format	dspace
spelling	oai:doaj.org-article:077cab94180b48079fe6484c8ab0ec502021-12-02T20:16:22ZTargeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.1932-620310.1371/journal.pone.0259165https://doaj.org/article/077cab94180b48079fe6484c8ab0ec502021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259165https://doaj.org/toc/1932-6203The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was estimated to be in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values <24 and in several samples with higher CT values. A clear relationship was observed between summed MS peak intensities for SARS-CoV-2 proteins and Ct values reflecting the abundance of viral RNA. Taken together, these results suggest that targeted MS based proteomics may have the potential to be used as an additional tool in COVID-19 diagnostics.Karel BezstarostiMart M LamersWouter A S DoffPeter C WeverKhoa T D ThaiJeroen J A van KampenBart L HaagmansJeroen A A DemmersPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259165 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Karel Bezstarosti Mart M Lamers Wouter A S Doff Peter C Wever Khoa T D Thai Jeroen J A van Kampen Bart L Haagmans Jeroen A A Demmers Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.
description	The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was estimated to be in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values <24 and in several samples with higher CT values. A clear relationship was observed between summed MS peak intensities for SARS-CoV-2 proteins and Ct values reflecting the abundance of viral RNA. Taken together, these results suggest that targeted MS based proteomics may have the potential to be used as an additional tool in COVID-19 diagnostics.
format	article
author	Karel Bezstarosti Mart M Lamers Wouter A S Doff Peter C Wever Khoa T D Thai Jeroen J A van Kampen Bart L Haagmans Jeroen A A Demmers
author_facet	Karel Bezstarosti Mart M Lamers Wouter A S Doff Peter C Wever Khoa T D Thai Jeroen J A van Kampen Bart L Haagmans Jeroen A A Demmers
author_sort	Karel Bezstarosti
title	Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.
title_short	Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.
title_full	Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.
title_fullStr	Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.
title_full_unstemmed	Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.
title_sort	targeted proteomics as a tool to detect sars-cov-2 proteins in clinical specimens.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/077cab94180b48079fe6484c8ab0ec50
work_keys_str_mv	AT karelbezstarosti targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens AT martmlamers targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens AT wouterasdoff targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens AT petercwever targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens AT khoatdthai targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens AT jeroenjavankampen targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens AT bartlhaagmans targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens AT jeroenaademmers targetedproteomicsasatooltodetectsarscov2proteinsinclinicalspecimens
_version_	1718374447775219712

Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens.

Ejemplares similares