TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano

Abstract TIM29 is a mitochondrial inner membrane protein that interacts with the protein import complex TIM22. TIM29 was shown to stabilize the TIM22 complex but its biological function remains largely unknown. Until recently, it was classified as one of the Domain of Unknown Function (DUF) genes, w...

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Autores principales:	Stijn Mouton, Kirill Ustyantsev, Frank Beltman, Lisa Glazenburg, Eugene Berezikov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0781953ca2a7452aba463a4374b11e70
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spelling	oai:doaj.org-article:0781953ca2a7452aba463a4374b11e702021-12-02T14:12:47ZTIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano10.1038/s41598-020-80682-72045-2322https://doaj.org/article/0781953ca2a7452aba463a4374b11e702021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80682-7https://doaj.org/toc/2045-2322Abstract TIM29 is a mitochondrial inner membrane protein that interacts with the protein import complex TIM22. TIM29 was shown to stabilize the TIM22 complex but its biological function remains largely unknown. Until recently, it was classified as one of the Domain of Unknown Function (DUF) genes, with a conserved protein domain DUF2366 of unclear function. Since characterizing DUF genes can provide novel biological insight, we used previously established transcriptional profiles of the germline and stem cells of the flatworm Macrostomum lignano to probe conserved DUFs for their potential role in germline biology, stem cell function, regeneration, and development. Here, we demonstrate that DUF2366/TIM29 knockdown in M. lignano has very limited effect during the normal homeostatic condition but prevents worms from adapting to a highly proliferative state required for regeneration.Stijn MoutonKirill UstyantsevFrank BeltmanLisa GlazenburgEugene BerezikovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stijn Mouton Kirill Ustyantsev Frank Beltman Lisa Glazenburg Eugene Berezikov TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano
description	Abstract TIM29 is a mitochondrial inner membrane protein that interacts with the protein import complex TIM22. TIM29 was shown to stabilize the TIM22 complex but its biological function remains largely unknown. Until recently, it was classified as one of the Domain of Unknown Function (DUF) genes, with a conserved protein domain DUF2366 of unclear function. Since characterizing DUF genes can provide novel biological insight, we used previously established transcriptional profiles of the germline and stem cells of the flatworm Macrostomum lignano to probe conserved DUFs for their potential role in germline biology, stem cell function, regeneration, and development. Here, we demonstrate that DUF2366/TIM29 knockdown in M. lignano has very limited effect during the normal homeostatic condition but prevents worms from adapting to a highly proliferative state required for regeneration.
format	article
author	Stijn Mouton Kirill Ustyantsev Frank Beltman Lisa Glazenburg Eugene Berezikov
author_facet	Stijn Mouton Kirill Ustyantsev Frank Beltman Lisa Glazenburg Eugene Berezikov
author_sort	Stijn Mouton
title	TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano
title_short	TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano
title_full	TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano
title_fullStr	TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano
title_full_unstemmed	TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano
title_sort	tim29 is required for enhanced stem cell activity during regeneration in the flatworm macrostomum lignano
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/0781953ca2a7452aba463a4374b11e70
work_keys_str_mv	AT stijnmouton tim29isrequiredforenhancedstemcellactivityduringregenerationintheflatwormmacrostomumlignano AT kirillustyantsev tim29isrequiredforenhancedstemcellactivityduringregenerationintheflatwormmacrostomumlignano AT frankbeltman tim29isrequiredforenhancedstemcellactivityduringregenerationintheflatwormmacrostomumlignano AT lisaglazenburg tim29isrequiredforenhancedstemcellactivityduringregenerationintheflatwormmacrostomumlignano AT eugeneberezikov tim29isrequiredforenhancedstemcellactivityduringregenerationintheflatwormmacrostomumlignano
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TIM29 is required for enhanced stem cell activity during regeneration in the flatworm Macrostomum lignano

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