Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus

Abstract. Adji A, Niode NJ, Memah VV, Posangi J, Wahongan GJP, Tallei TE. 2021. Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus. Biodiversitas 22: 72-78. Gene polymorphisms in Dermatophagoides pteronyssinus (DP) mite can affect the immune response that plays a r...

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Autores principales:	Aryani Adji, NURDJANNAH J. NIODE, VENTJE V. MEMAH, JIMMY POSANGI, GRETA J. P. WAHONGAN, TRINA E. TALLEI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	dermatophagoides pteronyssinus, mite, allergen, der p 1 gene, polymorphism Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/0782e7a389154e41b08e90d4dfebf9da
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spelling	oai:doaj.org-article:0782e7a389154e41b08e90d4dfebf9da2021-11-22T00:53:57ZDer p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus1412-033X2085-472210.13057/biodiv/d220110https://doaj.org/article/0782e7a389154e41b08e90d4dfebf9da2020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7049https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Adji A, Niode NJ, Memah VV, Posangi J, Wahongan GJP, Tallei TE. 2021. Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus. Biodiversitas 22: 72-78. Gene polymorphisms in Dermatophagoides pteronyssinus (DP) mite can affect the immune response that plays a role in atopic disease. This study aimed to determine the polymorphisms of Der p 1 gene from DP. House dust containing DP was used as a source of DNA containing the Der p 1 gene. The gene was amplified using specific primers DP Der 1 P 108F and DP Der 1 P 1509R. The amplified fragments were cloned using the Toyobo pTA2 vector and sorted then compared with the Gene Bank database. The result shows that there are 8 polymorphism sites in the Der p 1 gene positioned at 190 (GR), 463 (CT), 645 (AR), 751 (CY, CT), 787 (CG), 827 (TA; TW), and 1084 (CG). In the Der p 1 gene, there are 5 silent mutations and 3 nonsynonymous substitutions, resulting in amino acid polymorphisms as follows: Histidine H146 to Tyrosine Y146, Alanine A220 to Valine V220 and amino acid X220, and Tyrosine T232 to Serine S232. Polymorphisms in these amino acids lead to a polarity change, from polar to polar and non-polar to non-polar. These dynamics in polarity does not change the structure or function of the allergen protein. So that they can be used for vaccine design strategies.Aryani AdjiNURDJANNAH J. NIODEVENTJE V. MEMAHJIMMY POSANGIGRETA J. P. WAHONGANTRINA E. TALLEIMBI & UNS Soloarticledermatophagoides pteronyssinus, mite, allergen, der p 1 gene, polymorphismBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	dermatophagoides pteronyssinus, mite, allergen, der p 1 gene, polymorphism Biology (General) QH301-705.5
spellingShingle	dermatophagoides pteronyssinus, mite, allergen, der p 1 gene, polymorphism Biology (General) QH301-705.5 Aryani Adji NURDJANNAH J. NIODE VENTJE V. MEMAH JIMMY POSANGI GRETA J. P. WAHONGAN TRINA E. TALLEI Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus
description	Abstract. Adji A, Niode NJ, Memah VV, Posangi J, Wahongan GJP, Tallei TE. 2021. Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus. Biodiversitas 22: 72-78. Gene polymorphisms in Dermatophagoides pteronyssinus (DP) mite can affect the immune response that plays a role in atopic disease. This study aimed to determine the polymorphisms of Der p 1 gene from DP. House dust containing DP was used as a source of DNA containing the Der p 1 gene. The gene was amplified using specific primers DP Der 1 P 108F and DP Der 1 P 1509R. The amplified fragments were cloned using the Toyobo pTA2 vector and sorted then compared with the Gene Bank database. The result shows that there are 8 polymorphism sites in the Der p 1 gene positioned at 190 (GR), 463 (CT), 645 (AR), 751 (CY, CT), 787 (CG), 827 (TA; TW), and 1084 (CG). In the Der p 1 gene, there are 5 silent mutations and 3 nonsynonymous substitutions, resulting in amino acid polymorphisms as follows: Histidine H146 to Tyrosine Y146, Alanine A220 to Valine V220 and amino acid X220, and Tyrosine T232 to Serine S232. Polymorphisms in these amino acids lead to a polarity change, from polar to polar and non-polar to non-polar. These dynamics in polarity does not change the structure or function of the allergen protein. So that they can be used for vaccine design strategies.
format	article
author	Aryani Adji NURDJANNAH J. NIODE VENTJE V. MEMAH JIMMY POSANGI GRETA J. P. WAHONGAN TRINA E. TALLEI
author_facet	Aryani Adji NURDJANNAH J. NIODE VENTJE V. MEMAH JIMMY POSANGI GRETA J. P. WAHONGAN TRINA E. TALLEI
author_sort	Aryani Adji
title	Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus
title_short	Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus
title_full	Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus
title_fullStr	Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus
title_full_unstemmed	Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus
title_sort	der p 1 gene sequence polymorphism in house dust mite dermatophagoides pteronyssinus
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/0782e7a389154e41b08e90d4dfebf9da
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Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus

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