Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells

Spinosad, a neurotoxic insecticide, is widely used for crop protection. In order to elucidate the effects of spinosad on oxidative stress and genotoxicity in Sf9 cells, the levels of lipid peroxidation, the activity of antioxidative enzymes, and DNA damage were measured. The results showed that spin...

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Autores principales:	Wenping Xu, Mingjun Yang, Jufang Gao, Yang Zhang, Liming Tao
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	oxidative stress dna damage spinosad sf9 cells Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/07887eef096947e29550bc4f3f4edc11
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spelling	oai:doaj.org-article:07887eef096947e29550bc4f3f4edc112021-11-26T11:19:46ZOxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells0954-01051465-344310.1080/09540105.2017.1364708https://doaj.org/article/07887eef096947e29550bc4f3f4edc112018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1364708https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443Spinosad, a neurotoxic insecticide, is widely used for crop protection. In order to elucidate the effects of spinosad on oxidative stress and genotoxicity in Sf9 cells, the levels of lipid peroxidation, the activity of antioxidative enzymes, and DNA damage were measured. The results showed that spinosad caused a time-dependent increase in the formation of malondialdehyde and decrease in the activity of superoxide dismutase and catalase. Further studies confirmed that spinosad induced 8-oxoguanine accumulation in Sf9 cells, which is accompanied by increased expression of DNA repair enzymes (OGG1 and MTH1). The neutral comet assay revealed that spinosad induced significant time-related increases of DNA double-strand breaks in Sf9 cells. Our results indicate that spinosad effectively induced oxidative stress and DNA damage in Sf9 cells.Wenping XuMingjun YangJufang GaoYang ZhangLiming TaoTaylor & Francis Grouparticleoxidative stressdna damagespinosadsf9 cellsAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 171-181 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	oxidative stress dna damage spinosad sf9 cells Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	oxidative stress dna damage spinosad sf9 cells Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Wenping Xu Mingjun Yang Jufang Gao Yang Zhang Liming Tao Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
description	Spinosad, a neurotoxic insecticide, is widely used for crop protection. In order to elucidate the effects of spinosad on oxidative stress and genotoxicity in Sf9 cells, the levels of lipid peroxidation, the activity of antioxidative enzymes, and DNA damage were measured. The results showed that spinosad caused a time-dependent increase in the formation of malondialdehyde and decrease in the activity of superoxide dismutase and catalase. Further studies confirmed that spinosad induced 8-oxoguanine accumulation in Sf9 cells, which is accompanied by increased expression of DNA repair enzymes (OGG1 and MTH1). The neutral comet assay revealed that spinosad induced significant time-related increases of DNA double-strand breaks in Sf9 cells. Our results indicate that spinosad effectively induced oxidative stress and DNA damage in Sf9 cells.
format	article
author	Wenping Xu Mingjun Yang Jufang Gao Yang Zhang Liming Tao
author_facet	Wenping Xu Mingjun Yang Jufang Gao Yang Zhang Liming Tao
author_sort	Wenping Xu
title	Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
title_short	Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
title_full	Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
title_fullStr	Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
title_full_unstemmed	Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells
title_sort	oxidative stress and dna damage induced by spinosad exposure in spodoptera frugiperda sf9 cells
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/07887eef096947e29550bc4f3f4edc11
work_keys_str_mv	AT wenpingxu oxidativestressanddnadamageinducedbyspinosadexposureinspodopterafrugiperdasf9cells AT mingjunyang oxidativestressanddnadamageinducedbyspinosadexposureinspodopterafrugiperdasf9cells AT jufanggao oxidativestressanddnadamageinducedbyspinosadexposureinspodopterafrugiperdasf9cells AT yangzhang oxidativestressanddnadamageinducedbyspinosadexposureinspodopterafrugiperdasf9cells AT limingtao oxidativestressanddnadamageinducedbyspinosadexposureinspodopterafrugiperdasf9cells
_version_	1718409516395003904

Oxidative stress and DNA damage induced by spinosad exposure in Spodoptera frugiperda Sf9 cells

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