Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots

Isolation of individual cells from mixed populations is desirable for many biomedical applications. Here the authors use photoswitchable Pdots to allow 'optical painting', where cells of interest are marked based on their visual characteristics, and can then be isolated by fluorescence act...

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Autores principales: Chun-Ting Kuo, Alison M. Thompson, Maria Elena Gallina, Fangmao Ye, Eleanor S. Johnson, Wei Sun, Mengxia Zhao, Jiangbo Yu, I-Che Wu, Bryant Fujimoto, Christopher C. DuFort, Markus A. Carlson, Sunil R. Hingorani, Amy L. Paguirigan, Jerald P. Radich, Daniel T. Chiu
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2016
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Acceso en línea:https://doaj.org/article/079bb7e0e37b47bdb03eb5a552a99f85
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Sumario:Isolation of individual cells from mixed populations is desirable for many biomedical applications. Here the authors use photoswitchable Pdots to allow 'optical painting', where cells of interest are marked based on their visual characteristics, and can then be isolated by fluorescence activated cell sorting.