Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots
Isolation of individual cells from mixed populations is desirable for many biomedical applications. Here the authors use photoswitchable Pdots to allow 'optical painting', where cells of interest are marked based on their visual characteristics, and can then be isolated by fluorescence act...
Guardado en:
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2016
|
Materias: | |
Acceso en línea: | https://doaj.org/article/079bb7e0e37b47bdb03eb5a552a99f85 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:079bb7e0e37b47bdb03eb5a552a99f85 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:079bb7e0e37b47bdb03eb5a552a99f852021-12-02T15:35:18ZOptical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots10.1038/ncomms114682041-1723https://doaj.org/article/079bb7e0e37b47bdb03eb5a552a99f852016-04-01T00:00:00Zhttps://doi.org/10.1038/ncomms11468https://doaj.org/toc/2041-1723Isolation of individual cells from mixed populations is desirable for many biomedical applications. Here the authors use photoswitchable Pdots to allow 'optical painting', where cells of interest are marked based on their visual characteristics, and can then be isolated by fluorescence activated cell sorting.Chun-Ting KuoAlison M. ThompsonMaria Elena GallinaFangmao YeEleanor S. JohnsonWei SunMengxia ZhaoJiangbo YuI-Che WuBryant FujimotoChristopher C. DuFortMarkus A. CarlsonSunil R. HingoraniAmy L. PaguiriganJerald P. RadichDaniel T. ChiuNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-11 (2016) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Science Q |
spellingShingle |
Science Q Chun-Ting Kuo Alison M. Thompson Maria Elena Gallina Fangmao Ye Eleanor S. Johnson Wei Sun Mengxia Zhao Jiangbo Yu I-Che Wu Bryant Fujimoto Christopher C. DuFort Markus A. Carlson Sunil R. Hingorani Amy L. Paguirigan Jerald P. Radich Daniel T. Chiu Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots |
description |
Isolation of individual cells from mixed populations is desirable for many biomedical applications. Here the authors use photoswitchable Pdots to allow 'optical painting', where cells of interest are marked based on their visual characteristics, and can then be isolated by fluorescence activated cell sorting. |
format |
article |
author |
Chun-Ting Kuo Alison M. Thompson Maria Elena Gallina Fangmao Ye Eleanor S. Johnson Wei Sun Mengxia Zhao Jiangbo Yu I-Che Wu Bryant Fujimoto Christopher C. DuFort Markus A. Carlson Sunil R. Hingorani Amy L. Paguirigan Jerald P. Radich Daniel T. Chiu |
author_facet |
Chun-Ting Kuo Alison M. Thompson Maria Elena Gallina Fangmao Ye Eleanor S. Johnson Wei Sun Mengxia Zhao Jiangbo Yu I-Che Wu Bryant Fujimoto Christopher C. DuFort Markus A. Carlson Sunil R. Hingorani Amy L. Paguirigan Jerald P. Radich Daniel T. Chiu |
author_sort |
Chun-Ting Kuo |
title |
Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots |
title_short |
Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots |
title_full |
Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots |
title_fullStr |
Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots |
title_full_unstemmed |
Optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable Pdots |
title_sort |
optical painting and fluorescence activated sorting of single adherent cells labelled with photoswitchable pdots |
publisher |
Nature Portfolio |
publishDate |
2016 |
url |
https://doaj.org/article/079bb7e0e37b47bdb03eb5a552a99f85 |
work_keys_str_mv |
AT chuntingkuo opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT alisonmthompson opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT mariaelenagallina opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT fangmaoye opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT eleanorsjohnson opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT weisun opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT mengxiazhao opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT jiangboyu opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT ichewu opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT bryantfujimoto opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT christophercdufort opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT markusacarlson opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT sunilrhingorani opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT amylpaguirigan opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT jeraldpradich opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots AT danieltchiu opticalpaintingandfluorescenceactivatedsortingofsingleadherentcellslabelledwithphotoswitchablepdots |
_version_ |
1718386576685268992 |