Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure

Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Yuwono T. 2020. Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure. Biodiversitas 21: 3380-3390. The goal of the research was to determine the antimicrobial a...

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Autores principales:	Ambarwati Ambarwati, Subagus Wahyuono, Sukarti Moeljopawiro, Triwibowo Yuwono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	streptomyces, cyperus rotundus l., rhizosphere, antimicrobial, antismash Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/07ac6190a62a43de95abe5730ff0e4ac
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spelling	oai:doaj.org-article:07ac6190a62a43de95abe5730ff0e4ac2021-11-22T00:40:51ZAntimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure1412-033X2085-472210.13057/biodiv/d210763https://doaj.org/article/07ac6190a62a43de95abe5730ff0e4ac2020-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5687https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Yuwono T. 2020. Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure. Biodiversitas 21: 3380-3390. The goal of the research was to determine the antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and predict their chemical structure of the bioactive compounds based on the AntiSMASH analysis of the whole genome sequencing. Streptomyces sp. CRB46 was isolated from Cyperus rotundus L. rhizosphere in the Cemoro Sewu highland, Indonesia (1920 meters above sea level). The results showed that the supernatant ethyl acetate (EAS) extract could inhibit 11 of 12 tested microorganisms with a diameter of the inhibition zone ranging from 17-35 mm (paper disc method). The supernatant extract was found more effective in inhibiting microorganisms than the ethyl acetate extract of pellets (EAP). The EAP extract was only able to inhibit the growth of seven tested microorganisms (16-26 mm). The results of LCMS analysis showed that crude extract of EAS consisted of 20 mixtures of organic compounds. Based on FTIR analysis, 16 functional groups were found in the EAS extracts. In addition, based on AntiSMASH analysis, it was also found that Streptomyces sp. CRB46 produced eight classes of bioactive compounds, but only six compounds have been identified as their chemical structures. It can, therefore, be concluded that Streptomyces sp. CRB46 has the potential to produce bioactive compounds that can be used for microbial infection treatment.Ambarwati AmbarwatiSubagus WahyuonoSukarti MoeljopawiroTriwibowo YuwonoMBI & UNS Soloarticlestreptomyces, cyperus rotundus l., rhizosphere, antimicrobial, antismashBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 7 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	streptomyces, cyperus rotundus l., rhizosphere, antimicrobial, antismash Biology (General) QH301-705.5
spellingShingle	streptomyces, cyperus rotundus l., rhizosphere, antimicrobial, antismash Biology (General) QH301-705.5 Ambarwati Ambarwati Subagus Wahyuono Sukarti Moeljopawiro Triwibowo Yuwono Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure
description	Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Yuwono T. 2020. Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure. Biodiversitas 21: 3380-3390. The goal of the research was to determine the antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and predict their chemical structure of the bioactive compounds based on the AntiSMASH analysis of the whole genome sequencing. Streptomyces sp. CRB46 was isolated from Cyperus rotundus L. rhizosphere in the Cemoro Sewu highland, Indonesia (1920 meters above sea level). The results showed that the supernatant ethyl acetate (EAS) extract could inhibit 11 of 12 tested microorganisms with a diameter of the inhibition zone ranging from 17-35 mm (paper disc method). The supernatant extract was found more effective in inhibiting microorganisms than the ethyl acetate extract of pellets (EAP). The EAP extract was only able to inhibit the growth of seven tested microorganisms (16-26 mm). The results of LCMS analysis showed that crude extract of EAS consisted of 20 mixtures of organic compounds. Based on FTIR analysis, 16 functional groups were found in the EAS extracts. In addition, based on AntiSMASH analysis, it was also found that Streptomyces sp. CRB46 produced eight classes of bioactive compounds, but only six compounds have been identified as their chemical structures. It can, therefore, be concluded that Streptomyces sp. CRB46 has the potential to produce bioactive compounds that can be used for microbial infection treatment.
format	article
author	Ambarwati Ambarwati Subagus Wahyuono Sukarti Moeljopawiro Triwibowo Yuwono
author_facet	Ambarwati Ambarwati Subagus Wahyuono Sukarti Moeljopawiro Triwibowo Yuwono
author_sort	Ambarwati Ambarwati
title	Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure
title_short	Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure
title_full	Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure
title_fullStr	Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure
title_full_unstemmed	Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure
title_sort	antimicrobial activity of ethyl acetate extracts of streptomyces sp. crb46 and the prediction of their bioactive compounds chemical structure
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/07ac6190a62a43de95abe5730ff0e4ac
work_keys_str_mv	AT ambarwatiambarwati antimicrobialactivityofethylacetateextractsofstreptomycesspcrb46andthepredictionoftheirbioactivecompoundschemicalstructure AT subaguswahyuono antimicrobialactivityofethylacetateextractsofstreptomycesspcrb46andthepredictionoftheirbioactivecompoundschemicalstructure AT sukartimoeljopawiro antimicrobialactivityofethylacetateextractsofstreptomycesspcrb46andthepredictionoftheirbioactivecompoundschemicalstructure AT triwibowoyuwono antimicrobialactivityofethylacetateextractsofstreptomycesspcrb46andthepredictionoftheirbioactivecompoundschemicalstructure
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Antimicrobial activity of ethyl acetate extracts of Streptomyces sp. CRB46 and the prediction of their bioactive compounds chemical structure

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