Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients

Tingting Wu,&ast; Dandan Xie,&ast; Xiaotong Zhao, Murong Xu, Li Luo, Datong Deng, Mingwei Chen Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230032, People’s Republic of China&ast;These authors contributed equally to this workCorres...

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Autores principales:	Wu T, Xie D, Zhao X, Xu M, Luo L, Deng D, Chen M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	type 2 diebetes diabetic foot diabetic foot osteomyelitis mirnas Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/07af333a2c20487cb42befead13adf3c
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spelling	oai:doaj.org-article:07af333a2c20487cb42befead13adf3c2021-12-02T17:00:53ZEnhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients1178-7007https://doaj.org/article/07af333a2c20487cb42befead13adf3c2021-10-01T00:00:00Zhttps://www.dovepress.com/enhanced-expression-of-mir-34c-in-peripheral-plasma-associated-with-di-peer-reviewed-fulltext-article-DMSOhttps://doaj.org/toc/1178-7007Tingting Wu,&ast; Dandan Xie,&ast; Xiaotong Zhao, Murong Xu, Li Luo, Datong Deng, Mingwei Chen Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230032, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Mingwei ChenDepartment of Endocrinology, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, Anhui, 230032, People’s Republic of ChinaEmail chmw1@163.comObjective: To explore the correlation between the expression of miR-34c in peripheral blood of patients with type 2 diabetes mellitus (T2DM) and the onset of diabetic foot ulcer (DFU) and diabetic foot osteomyelitis (DFO).Methods: Sixty newly diagnosed patients with T2DM without DFU (T2DM group), 112 T2DM patients with DFU (DFU group) and 60 controls with normal glucose tolerance (NC group). The DFU group patients were subdivided into DFO (n=64) and NDFO (n=48) groups. Quantitative real-time PCR (qRT-PCR) method was used to determine miR-34c expression levels in the peripheral blood of subjects to analyze the clinical characteristics of DFU and DFO risk factors.Results: MiR-34c expression level in the T2DM group was marked higher than the NC group [2.99 (1.45– 6.22) vs 1.01 (0.89– 1.52)] (P < 0.05). However, the expression level of miR-34c in the DFU group was significantly higher than the T2DM group [9.65 (6.15– 18.63) vs 2.99 (1.45– 6.22)] (P < 0.01). Compared with the NDFO group, the expression level of miR-34c in the DFO group was also obviously increased [13.46 (8.89– 19.11) vs 6.02 (5.93– 14.72)] (P < 0.01). The expression level of miR-34c in DFU patients was positively correlated with the amputation rate of foot ulcers (P=0.030) and was negatively correlated with the healing rate of foot ulcers after eight weeks (P=0.025). Multifactorial logistic regression analysis showed that increased expression of miR-34c was an independent risk factor for DFU and DFO (ORDFU=3.47, ORDFO=4.25, P < 0.01). Meanwhile, the ROC curve analysis indicated that the AUC of miR-34c for the diagnosis of DFU and DFO was 0.803 and 0.904, the optimum sensitivity being was 100% and 98.7%, the optimum specificity was 98.4% and 98.4%, respectively.Conclusion: The increased expression of miR-34c in peripheral blood of T2DM patients is closely related to the occurrence, development and prognosis of DFU and DFO.Keywords: type 2 diabetes, diabetic foot, diabetic foot osteomyelitis, miRNAsWu TXie DZhao XXu MLuo LDeng DChen MDove Medical Pressarticletype 2 diebetesdiabetic footdiabetic foot osteomyelitismirnasSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 4263-4273 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	type 2 diebetes diabetic foot diabetic foot osteomyelitis mirnas Specialties of internal medicine RC581-951
spellingShingle	type 2 diebetes diabetic foot diabetic foot osteomyelitis mirnas Specialties of internal medicine RC581-951 Wu T Xie D Zhao X Xu M Luo L Deng D Chen M Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients
description	Tingting Wu,&ast; Dandan Xie,&ast; Xiaotong Zhao, Murong Xu, Li Luo, Datong Deng, Mingwei Chen Department of Endocrinology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230032, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Mingwei ChenDepartment of Endocrinology, The First Affiliated Hospital of Anhui Medical University, No. 218 Jixi Road, Hefei, Anhui, 230032, People’s Republic of ChinaEmail chmw1@163.comObjective: To explore the correlation between the expression of miR-34c in peripheral blood of patients with type 2 diabetes mellitus (T2DM) and the onset of diabetic foot ulcer (DFU) and diabetic foot osteomyelitis (DFO).Methods: Sixty newly diagnosed patients with T2DM without DFU (T2DM group), 112 T2DM patients with DFU (DFU group) and 60 controls with normal glucose tolerance (NC group). The DFU group patients were subdivided into DFO (n=64) and NDFO (n=48) groups. Quantitative real-time PCR (qRT-PCR) method was used to determine miR-34c expression levels in the peripheral blood of subjects to analyze the clinical characteristics of DFU and DFO risk factors.Results: MiR-34c expression level in the T2DM group was marked higher than the NC group [2.99 (1.45– 6.22) vs 1.01 (0.89– 1.52)] (P < 0.05). However, the expression level of miR-34c in the DFU group was significantly higher than the T2DM group [9.65 (6.15– 18.63) vs 2.99 (1.45– 6.22)] (P < 0.01). Compared with the NDFO group, the expression level of miR-34c in the DFO group was also obviously increased [13.46 (8.89– 19.11) vs 6.02 (5.93– 14.72)] (P < 0.01). The expression level of miR-34c in DFU patients was positively correlated with the amputation rate of foot ulcers (P=0.030) and was negatively correlated with the healing rate of foot ulcers after eight weeks (P=0.025). Multifactorial logistic regression analysis showed that increased expression of miR-34c was an independent risk factor for DFU and DFO (ORDFU=3.47, ORDFO=4.25, P < 0.01). Meanwhile, the ROC curve analysis indicated that the AUC of miR-34c for the diagnosis of DFU and DFO was 0.803 and 0.904, the optimum sensitivity being was 100% and 98.7%, the optimum specificity was 98.4% and 98.4%, respectively.Conclusion: The increased expression of miR-34c in peripheral blood of T2DM patients is closely related to the occurrence, development and prognosis of DFU and DFO.Keywords: type 2 diabetes, diabetic foot, diabetic foot osteomyelitis, miRNAs
format	article
author	Wu T Xie D Zhao X Xu M Luo L Deng D Chen M
author_facet	Wu T Xie D Zhao X Xu M Luo L Deng D Chen M
author_sort	Wu T
title	Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients
title_short	Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients
title_full	Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients
title_fullStr	Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients
title_full_unstemmed	Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients
title_sort	enhanced expression of mir-34c in peripheral plasma associated with diabetic foot ulcer in type 2 diabetes patients
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/07af333a2c20487cb42befead13adf3c
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Enhanced Expression of miR-34c in Peripheral Plasma Associated with Diabetic Foot Ulcer in Type 2 Diabetes Patients

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