Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Kai Sun, Yong Chang, Binbin Zhou, Xiaojin Wang, Lin Liu Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People’s Republic of China Abstract: This article presents a genera...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sun K, Chang Y, Zhou B, Wang X, Liu L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Electrochemical biosensor colorimetric assay gold nanoparticle aptameric peptide protein kinase A signal amplification Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/07b6e6a257d14bc4be2ed55926a2a547
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:07b6e6a257d14bc4be2ed55926a2a547
record_format	dspace
spelling	oai:doaj.org-article:07b6e6a257d14bc4be2ed55926a2a5472021-12-02T01:34:06ZGold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor1178-2013https://doaj.org/article/07b6e6a257d14bc4be2ed55926a2a5472017-03-01T00:00:00Zhttps://www.dovepress.com/gold-nanoparticles-based-electrochemical-method-for-the-detection-of-p-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Kai Sun, Yong Chang, Binbin Zhou, Xiaojin Wang, Lin Liu Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People’s Republic of China Abstract: This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold nanoparticles (AuNPs)-based colorimetric assay into sensitive electrochemical analysis. In the colorimetric assay, the kinase-specific aptameric peptide triggered the aggregation of AuNPs in solution. However, the specific binding of peptide to the target protein (kinase) inhibited its ability to trigger the assembly of AuNPs. In the electrochemical analysis, peptides immobilized on a gold electrode and presented as solution triggered together the in situ formation of AuNPs-based network architecture on the electrode surface. Nevertheless, the formation of peptide–kinase complex on the electrode surface made the peptide-triggered AuNPs assembly difficult. Electrochemical impedance spectroscopy was used to measure the change in surface property in the binding events. When a ferrocene-labeled peptide (Fc-peptide) was used in this design, the network of AuNPs/Fc-peptide produced a good voltammetric signal. The competitive assay allowed for the detection of protein kinase A with a detection limit of 20 mU/mL. This work should be valuable for designing novel optical or electronic biosensors and likely lead to many detection applications. Keywords: electrochemical biosensor, colorimetric assay, gold nanoparticle, aptameric peptide, protein kinase A, signal amplification Sun KChang YZhou BWang XLiu LDove Medical PressarticleElectrochemical biosensorcolorimetric assaygold nanoparticleaptameric peptideprotein kinase Asignal amplificationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1905-1915 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Electrochemical biosensor colorimetric assay gold nanoparticle aptameric peptide protein kinase A signal amplification Medicine (General) R5-920
spellingShingle	Electrochemical biosensor colorimetric assay gold nanoparticle aptameric peptide protein kinase A signal amplification Medicine (General) R5-920 Sun K Chang Y Zhou B Wang X Liu L Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor
description	Kai Sun, Yong Chang, Binbin Zhou, Xiaojin Wang, Lin Liu Henan Province of Key Laboratory of New Optoelectronic Functional Materials, College of Chemistry and Chemical Engineering, Anyang Normal University, Anyang, Henan, People’s Republic of China Abstract: This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold nanoparticles (AuNPs)-based colorimetric assay into sensitive electrochemical analysis. In the colorimetric assay, the kinase-specific aptameric peptide triggered the aggregation of AuNPs in solution. However, the specific binding of peptide to the target protein (kinase) inhibited its ability to trigger the assembly of AuNPs. In the electrochemical analysis, peptides immobilized on a gold electrode and presented as solution triggered together the in situ formation of AuNPs-based network architecture on the electrode surface. Nevertheless, the formation of peptide–kinase complex on the electrode surface made the peptide-triggered AuNPs assembly difficult. Electrochemical impedance spectroscopy was used to measure the change in surface property in the binding events. When a ferrocene-labeled peptide (Fc-peptide) was used in this design, the network of AuNPs/Fc-peptide produced a good voltammetric signal. The competitive assay allowed for the detection of protein kinase A with a detection limit of 20 mU/mL. This work should be valuable for designing novel optical or electronic biosensors and likely lead to many detection applications. Keywords: electrochemical biosensor, colorimetric assay, gold nanoparticle, aptameric peptide, protein kinase A, signal amplification
format	article
author	Sun K Chang Y Zhou B Wang X Liu L
author_facet	Sun K Chang Y Zhou B Wang X Liu L
author_sort	Sun K
title	Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor
title_short	Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor
title_full	Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor
title_fullStr	Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor
title_full_unstemmed	Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor
title_sort	gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/07b6e6a257d14bc4be2ed55926a2a547
work_keys_str_mv	AT sunk goldnanoparticlesbasedelectrochemicalmethodforthedetectionofproteinkinasewithapeptidelikeinhibitorasthebioreceptor AT changy goldnanoparticlesbasedelectrochemicalmethodforthedetectionofproteinkinasewithapeptidelikeinhibitorasthebioreceptor AT zhoub goldnanoparticlesbasedelectrochemicalmethodforthedetectionofproteinkinasewithapeptidelikeinhibitorasthebioreceptor AT wangx goldnanoparticlesbasedelectrochemicalmethodforthedetectionofproteinkinasewithapeptidelikeinhibitorasthebioreceptor AT liul goldnanoparticlesbasedelectrochemicalmethodforthedetectionofproteinkinasewithapeptidelikeinhibitorasthebioreceptor
_version_	1718402996766769152

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Ejemplares similares