<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities

ABSTRACT Candida albicans is a fungus that colonizes oral cavity surfaces, the gut, and the genital tract. Streptococcus gordonii is a ubiquitous oral bacterium that has been shown to form biofilm communities with C. albicans. Formation of dual-species S. gordonii-C. albicans biofilm communities inv...

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Autores principales:	Lindsay C. Dutton, Angela H. Nobbs, Katy Jepson, Mark A. Jepson, M. Margaret Vickerman, Sami Aqeel Alawfi, Carol A. Munro, Richard J. Lamont, Howard F. Jenkinson
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2014
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/07c4261ac2b84ba6b459a69cc1ff14e4
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spelling	oai:doaj.org-article:07c4261ac2b84ba6b459a69cc1ff14e42021-11-15T15:45:13Z<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities10.1128/mBio.00911-142150-7511https://doaj.org/article/07c4261ac2b84ba6b459a69cc1ff14e42014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00911-14https://doaj.org/toc/2150-7511ABSTRACT Candida albicans is a fungus that colonizes oral cavity surfaces, the gut, and the genital tract. Streptococcus gordonii is a ubiquitous oral bacterium that has been shown to form biofilm communities with C. albicans. Formation of dual-species S. gordonii-C. albicans biofilm communities involves interaction of the S. gordonii SspB protein with the Als3 protein on the hyphal filament surface of C. albicans. Mannoproteins comprise a major component of the C. albicans cell wall, and in this study we sought to determine if mannosylation in cell wall biogenesis of C. albicans was necessary for hyphal adhesin functions associated with interkingdom biofilm development. A C. albicans mnt1Δ mnt2Δ mutant, with deleted α-1,2-mannosyltransferase genes and thus defective in O-mannosylation, was abrogated in biofilm formation under various growth conditions and produced hyphal filaments that were not recognized by S. gordonii. Cell wall proteomes of hypha-forming mnt1Δ mnt2Δ mutant cells showed growth medium-dependent alterations, compared to findings for the wild type, in a range of protein components, including Als1, Als3, Rbt1, Scw1, and Sap9. Hyphal filaments formed by mnt1Δ mnt2Δ mutant cells, unlike wild-type hyphae, did not interact with C. albicans Als3 or Hwp1 partner cell wall proteins or with S. gordonii SspB partner adhesin, suggesting defective functionality of adhesins on the mnt1Δ mnt2Δ mutant. These observations imply that early stage O-mannosylation is critical for activation of hyphal adhesin functions required for biofilm formation, recognition by bacteria such as S. gordonii, and microbial community development. IMPORTANCE In the human mouth, microorganisms form communities known as biofilms that adhere to the surfaces present. Candida albicans is a fungus that is often found within these biofilms. We have focused on the mechanisms by which C. albicans becomes incorporated into communities containing bacteria, such as Streptococcus. We find that impairment of early stage addition of mannose sugars to C. albicans hyphal filament proteins deleteriously affects their subsequent performance in mediating formation of polymicrobial biofilms. Our analyses provide new understanding of the way that microbial communities develop, and of potential means to control C. albicans infections.Lindsay C. DuttonAngela H. NobbsKaty JepsonMark A. JepsonM. Margaret VickermanSami Aqeel AlawfiCarol A. MunroRichard J. LamontHoward F. JenkinsonAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 5, Iss 2 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Lindsay C. Dutton Angela H. Nobbs Katy Jepson Mark A. Jepson M. Margaret Vickerman Sami Aqeel Alawfi Carol A. Munro Richard J. Lamont Howard F. Jenkinson <italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities
description	ABSTRACT Candida albicans is a fungus that colonizes oral cavity surfaces, the gut, and the genital tract. Streptococcus gordonii is a ubiquitous oral bacterium that has been shown to form biofilm communities with C. albicans. Formation of dual-species S. gordonii-C. albicans biofilm communities involves interaction of the S. gordonii SspB protein with the Als3 protein on the hyphal filament surface of C. albicans. Mannoproteins comprise a major component of the C. albicans cell wall, and in this study we sought to determine if mannosylation in cell wall biogenesis of C. albicans was necessary for hyphal adhesin functions associated with interkingdom biofilm development. A C. albicans mnt1Δ mnt2Δ mutant, with deleted α-1,2-mannosyltransferase genes and thus defective in O-mannosylation, was abrogated in biofilm formation under various growth conditions and produced hyphal filaments that were not recognized by S. gordonii. Cell wall proteomes of hypha-forming mnt1Δ mnt2Δ mutant cells showed growth medium-dependent alterations, compared to findings for the wild type, in a range of protein components, including Als1, Als3, Rbt1, Scw1, and Sap9. Hyphal filaments formed by mnt1Δ mnt2Δ mutant cells, unlike wild-type hyphae, did not interact with C. albicans Als3 or Hwp1 partner cell wall proteins or with S. gordonii SspB partner adhesin, suggesting defective functionality of adhesins on the mnt1Δ mnt2Δ mutant. These observations imply that early stage O-mannosylation is critical for activation of hyphal adhesin functions required for biofilm formation, recognition by bacteria such as S. gordonii, and microbial community development. IMPORTANCE In the human mouth, microorganisms form communities known as biofilms that adhere to the surfaces present. Candida albicans is a fungus that is often found within these biofilms. We have focused on the mechanisms by which C. albicans becomes incorporated into communities containing bacteria, such as Streptococcus. We find that impairment of early stage addition of mannose sugars to C. albicans hyphal filament proteins deleteriously affects their subsequent performance in mediating formation of polymicrobial biofilms. Our analyses provide new understanding of the way that microbial communities develop, and of potential means to control C. albicans infections.
format	article
author	Lindsay C. Dutton Angela H. Nobbs Katy Jepson Mark A. Jepson M. Margaret Vickerman Sami Aqeel Alawfi Carol A. Munro Richard J. Lamont Howard F. Jenkinson
author_facet	Lindsay C. Dutton Angela H. Nobbs Katy Jepson Mark A. Jepson M. Margaret Vickerman Sami Aqeel Alawfi Carol A. Munro Richard J. Lamont Howard F. Jenkinson
author_sort	Lindsay C. Dutton
title	<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities
title_short	<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities
title_full	<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities
title_fullStr	<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities
title_full_unstemmed	<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities
title_sort	<italic toggle="yes">o</italic>-mannosylation in <named-content content-type="genus-species">candida albicans</named-content> enables development of interkingdom biofilm communities
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/07c4261ac2b84ba6b459a69cc1ff14e4
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<italic toggle="yes">O</italic>-Mannosylation in <named-content content-type="genus-species">Candida albicans</named-content> Enables Development of Interkingdom Biofilm Communities

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