Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death.
<h4>Background</h4>Retinal detachment often leads to a severe and permanent loss of vision and its therapeutic management remains to this day exclusively surgical. We have used surgical specimens to perform a differential analysis of the transcriptome of human retinal tissues following d...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Public Library of Science (PLoS)
2011
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/07c8bb8cac074eb59cd7f7d7776adb4e |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:07c8bb8cac074eb59cd7f7d7776adb4e |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:07c8bb8cac074eb59cd7f7d7776adb4e2021-11-18T07:32:35ZTranscriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death.1932-620310.1371/journal.pone.0028791https://doaj.org/article/07c8bb8cac074eb59cd7f7d7776adb4e2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22174898/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Retinal detachment often leads to a severe and permanent loss of vision and its therapeutic management remains to this day exclusively surgical. We have used surgical specimens to perform a differential analysis of the transcriptome of human retinal tissues following detachment in order to identify new potential pharmacological targets that could be used in combination with surgery to further improve final outcome.<h4>Methodology/principal findings</h4>Statistical analysis reveals major involvement of the immune response in the disease. Interestingly, using a novel approach relying on coordinated expression, the interindividual variation was monitored to unravel a second crucial aspect of the pathological process: the death of photoreceptor cells. Within the genes identified, the expression of the major histocompatibility complex I gene HLA-C enables diagnosis of the disease, while PKD2L1 and SLCO4A1 -which are both down-regulated- act synergistically to provide an estimate of the duration of the retinal detachment process. Our analysis thus reveals the two complementary cellular and molecular aspects linked to retinal detachment: an immune response and the degeneration of photoreceptor cells. We also reveal that the human specimens have a higher clinical value as compared to artificial models that point to IL6 and oxidative stress, not implicated in the surgical specimens studied here.<h4>Conclusions/significance</h4>This systematic analysis confirmed the occurrence of both neurodegeneration and inflammation during retinal detachment, and further identifies precisely the modification of expression of the different genes implicated in these two phenomena. Our data henceforth give a new insight into the disease process and provide a rationale for therapeutic strategies aimed at limiting inflammation and photoreceptor damage associated with retinal detachment and, in turn, improving visual prognosis after retinal surgery.Marie-Noëlle DelyferWolfgang RaffelsbergerDavid MercierJean-François KorobelnikAlain GaudricDavid G CharterisRamin TadayoniFlorence MetgeGeorges CaputoPierre-Olivier BaraleRaymond RippJean-Denis MullerOlivier PochJosé-Alain SahelThierry LéveillardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 12, p e28791 (2011) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Marie-Noëlle Delyfer Wolfgang Raffelsberger David Mercier Jean-François Korobelnik Alain Gaudric David G Charteris Ramin Tadayoni Florence Metge Georges Caputo Pierre-Olivier Barale Raymond Ripp Jean-Denis Muller Olivier Poch José-Alain Sahel Thierry Léveillard Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death. |
| description |
<h4>Background</h4>Retinal detachment often leads to a severe and permanent loss of vision and its therapeutic management remains to this day exclusively surgical. We have used surgical specimens to perform a differential analysis of the transcriptome of human retinal tissues following detachment in order to identify new potential pharmacological targets that could be used in combination with surgery to further improve final outcome.<h4>Methodology/principal findings</h4>Statistical analysis reveals major involvement of the immune response in the disease. Interestingly, using a novel approach relying on coordinated expression, the interindividual variation was monitored to unravel a second crucial aspect of the pathological process: the death of photoreceptor cells. Within the genes identified, the expression of the major histocompatibility complex I gene HLA-C enables diagnosis of the disease, while PKD2L1 and SLCO4A1 -which are both down-regulated- act synergistically to provide an estimate of the duration of the retinal detachment process. Our analysis thus reveals the two complementary cellular and molecular aspects linked to retinal detachment: an immune response and the degeneration of photoreceptor cells. We also reveal that the human specimens have a higher clinical value as compared to artificial models that point to IL6 and oxidative stress, not implicated in the surgical specimens studied here.<h4>Conclusions/significance</h4>This systematic analysis confirmed the occurrence of both neurodegeneration and inflammation during retinal detachment, and further identifies precisely the modification of expression of the different genes implicated in these two phenomena. Our data henceforth give a new insight into the disease process and provide a rationale for therapeutic strategies aimed at limiting inflammation and photoreceptor damage associated with retinal detachment and, in turn, improving visual prognosis after retinal surgery. |
| format |
article |
| author |
Marie-Noëlle Delyfer Wolfgang Raffelsberger David Mercier Jean-François Korobelnik Alain Gaudric David G Charteris Ramin Tadayoni Florence Metge Georges Caputo Pierre-Olivier Barale Raymond Ripp Jean-Denis Muller Olivier Poch José-Alain Sahel Thierry Léveillard |
| author_facet |
Marie-Noëlle Delyfer Wolfgang Raffelsberger David Mercier Jean-François Korobelnik Alain Gaudric David G Charteris Ramin Tadayoni Florence Metge Georges Caputo Pierre-Olivier Barale Raymond Ripp Jean-Denis Muller Olivier Poch José-Alain Sahel Thierry Léveillard |
| author_sort |
Marie-Noëlle Delyfer |
| title |
Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death. |
| title_short |
Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death. |
| title_full |
Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death. |
| title_fullStr |
Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death. |
| title_full_unstemmed |
Transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death. |
| title_sort |
transcriptomic analysis of human retinal detachment reveals both inflammatory response and photoreceptor death. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2011 |
| url |
https://doaj.org/article/07c8bb8cac074eb59cd7f7d7776adb4e |
| work_keys_str_mv |
AT marienoelledelyfer transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT wolfgangraffelsberger transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT davidmercier transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT jeanfrancoiskorobelnik transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT alaingaudric transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT davidgcharteris transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT ramintadayoni transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT florencemetge transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT georgescaputo transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT pierreolivierbarale transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT raymondripp transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT jeandenismuller transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT olivierpoch transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT josealainsahel transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath AT thierryleveillard transcriptomicanalysisofhumanretinaldetachmentrevealsbothinflammatoryresponseandphotoreceptordeath |
| _version_ |
1718423266233679872 |