Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions

ABSTRACT Typically, established lab strains are widely used to study host-pathogen interactions. However, to better reflect the infection process, the experimental use of clinical isolates has come more into focus. Here, we analyzed the interaction of multiple vaginal isolates of the opportunistic f...

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Autores principales:	Franziska Gerwien, Christine Dunker, Philipp Brandt, Enrico Garbe, Ilse D. Jacobsen, Slavena Vylkova
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	Candida albicans vulvovaginal candidiasis macrophages cell wall Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/07ca3bfe98764c868e87612908843f05
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spelling	oai:doaj.org-article:07ca3bfe98764c868e87612908843f052021-11-15T15:30:51ZClinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions10.1128/mSphere.00393-202379-5042https://doaj.org/article/07ca3bfe98764c868e87612908843f052020-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00393-20https://doaj.org/toc/2379-5042ABSTRACT Typically, established lab strains are widely used to study host-pathogen interactions. However, to better reflect the infection process, the experimental use of clinical isolates has come more into focus. Here, we analyzed the interaction of multiple vaginal isolates of the opportunistic fungal pathogen Candida albicans, the most common cause of vulvovaginal candidiasis in women, with key players of the host immune system: macrophages. We tested several strains isolated from asymptomatic or symptomatic women with acute and recurrent infections. While all clinical strains showed a response similar to the commonly used lab strain SC5314 in various in vitro assays, they displayed remarkable differences during interaction with macrophages. This coincided with significantly reduced β-glucan exposure on the cell surface, which appeared to be a shared property among the tested vaginal strains for yeast extract/peptone/dextrose-grown cells, which is partly lost when the isolates faced vaginal niche-like nutrient conditions. However, macrophage damage, survival of phagocytosis, and filamentation capacities were highly strain-specific. These results highlight the high heterogeneity of C. albicans strains in host-pathogen interactions, which have to be taken into account to bridge the gap between laboratory-gained data and disease-related outcomes in an actual patient. IMPORTANCE Vulvovaginal candidiasis is one of the most common fungal infections in humans with Candida albicans as the major causative agent. This study is the first to compare clinical vaginal isolates of defined patient groups in their interaction with macrophages, highlighting the vastly different outcomes in comparison to a laboratory strain using commonly applied virulence-determining assays.Franziska GerwienChristine DunkerPhilipp BrandtEnrico GarbeIlse D. JacobsenSlavena VylkovaAmerican Society for MicrobiologyarticleCandida albicansvulvovaginal candidiasismacrophagescell wallMicrobiologyQR1-502ENmSphere, Vol 5, Iss 4 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Candida albicans vulvovaginal candidiasis macrophages cell wall Microbiology QR1-502
spellingShingle	Candida albicans vulvovaginal candidiasis macrophages cell wall Microbiology QR1-502 Franziska Gerwien Christine Dunker Philipp Brandt Enrico Garbe Ilse D. Jacobsen Slavena Vylkova Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions
description	ABSTRACT Typically, established lab strains are widely used to study host-pathogen interactions. However, to better reflect the infection process, the experimental use of clinical isolates has come more into focus. Here, we analyzed the interaction of multiple vaginal isolates of the opportunistic fungal pathogen Candida albicans, the most common cause of vulvovaginal candidiasis in women, with key players of the host immune system: macrophages. We tested several strains isolated from asymptomatic or symptomatic women with acute and recurrent infections. While all clinical strains showed a response similar to the commonly used lab strain SC5314 in various in vitro assays, they displayed remarkable differences during interaction with macrophages. This coincided with significantly reduced β-glucan exposure on the cell surface, which appeared to be a shared property among the tested vaginal strains for yeast extract/peptone/dextrose-grown cells, which is partly lost when the isolates faced vaginal niche-like nutrient conditions. However, macrophage damage, survival of phagocytosis, and filamentation capacities were highly strain-specific. These results highlight the high heterogeneity of C. albicans strains in host-pathogen interactions, which have to be taken into account to bridge the gap between laboratory-gained data and disease-related outcomes in an actual patient. IMPORTANCE Vulvovaginal candidiasis is one of the most common fungal infections in humans with Candida albicans as the major causative agent. This study is the first to compare clinical vaginal isolates of defined patient groups in their interaction with macrophages, highlighting the vastly different outcomes in comparison to a laboratory strain using commonly applied virulence-determining assays.
format	article
author	Franziska Gerwien Christine Dunker Philipp Brandt Enrico Garbe Ilse D. Jacobsen Slavena Vylkova
author_facet	Franziska Gerwien Christine Dunker Philipp Brandt Enrico Garbe Ilse D. Jacobsen Slavena Vylkova
author_sort	Franziska Gerwien
title	Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions
title_short	Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions
title_full	Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions
title_fullStr	Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions
title_full_unstemmed	Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions
title_sort	clinical <italic toggle="yes">candida albicans</italic> vaginal isolates and a laboratory strain show divergent behaviors during macrophage interactions
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/07ca3bfe98764c868e87612908843f05
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Clinical <italic toggle="yes">Candida albicans</italic> Vaginal Isolates and a Laboratory Strain Show Divergent Behaviors during Macrophage Interactions

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