Evidence for exocellular Arsenic in Fronds of Pteris vittata

Abstract The arsenic (As) hyperaccumulating fern species Pteris vittata (PV) is capable of accumulating large quantities of As in its aboveground tissues. Transformation to AsIII and vacuolar sequestration is believed to be the As detoxification mechanism in PV. Here we present evidence for a prepon...

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Autores principales:	Rupali Datta, Padmini Das, Ryan Tappero, Pravin Punamiya, Evert Elzinga, Shivendra Sahi, Huan Feng, Jeffrey Kiiskila, Dibyendu Sarkar
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07ce908e7339469db4c55fcbbe780781
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spelling	oai:doaj.org-article:07ce908e7339469db4c55fcbbe7807812021-12-02T16:07:00ZEvidence for exocellular Arsenic in Fronds of Pteris vittata10.1038/s41598-017-03194-x2045-2322https://doaj.org/article/07ce908e7339469db4c55fcbbe7807812017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03194-xhttps://doaj.org/toc/2045-2322Abstract The arsenic (As) hyperaccumulating fern species Pteris vittata (PV) is capable of accumulating large quantities of As in its aboveground tissues. Transformation to AsIII and vacuolar sequestration is believed to be the As detoxification mechanism in PV. Here we present evidence for a preponderance of exocellular As in fronds of Pteris vittata despite numerous reports of a tolerance mechanism involving intracellular compartmentalization. Results of an extraction experiment show that 43–71% of the As extruded out of the fronds of PV grown in 0.67, 3.3 and 6.7 mM AsV. SEM-EDX analysis showed that As was localized largely on the lower pinna surface, with smaller amounts on the upper surface, as crystalline deposits. X-ray fluorescence imaging of pinna cross-sections revealed preferential localization of As on the pinna surface in the proximity of veins, with the majority localized near the midrib. Majority of the As in the pinnae is contained in the apoplast rather than vacuoles. Our results provide evidence that exocellular sequestration is potentially a mechanism of As detoxification in PV, particularly at higher As concentrations, raising concern about its use for phytoremediation.Rupali DattaPadmini DasRyan TapperoPravin PunamiyaEvert ElzingaShivendra SahiHuan FengJeffrey KiiskilaDibyendu SarkarNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rupali Datta Padmini Das Ryan Tappero Pravin Punamiya Evert Elzinga Shivendra Sahi Huan Feng Jeffrey Kiiskila Dibyendu Sarkar Evidence for exocellular Arsenic in Fronds of Pteris vittata
description	Abstract The arsenic (As) hyperaccumulating fern species Pteris vittata (PV) is capable of accumulating large quantities of As in its aboveground tissues. Transformation to AsIII and vacuolar sequestration is believed to be the As detoxification mechanism in PV. Here we present evidence for a preponderance of exocellular As in fronds of Pteris vittata despite numerous reports of a tolerance mechanism involving intracellular compartmentalization. Results of an extraction experiment show that 43–71% of the As extruded out of the fronds of PV grown in 0.67, 3.3 and 6.7 mM AsV. SEM-EDX analysis showed that As was localized largely on the lower pinna surface, with smaller amounts on the upper surface, as crystalline deposits. X-ray fluorescence imaging of pinna cross-sections revealed preferential localization of As on the pinna surface in the proximity of veins, with the majority localized near the midrib. Majority of the As in the pinnae is contained in the apoplast rather than vacuoles. Our results provide evidence that exocellular sequestration is potentially a mechanism of As detoxification in PV, particularly at higher As concentrations, raising concern about its use for phytoremediation.
format	article
author	Rupali Datta Padmini Das Ryan Tappero Pravin Punamiya Evert Elzinga Shivendra Sahi Huan Feng Jeffrey Kiiskila Dibyendu Sarkar
author_facet	Rupali Datta Padmini Das Ryan Tappero Pravin Punamiya Evert Elzinga Shivendra Sahi Huan Feng Jeffrey Kiiskila Dibyendu Sarkar
author_sort	Rupali Datta
title	Evidence for exocellular Arsenic in Fronds of Pteris vittata
title_short	Evidence for exocellular Arsenic in Fronds of Pteris vittata
title_full	Evidence for exocellular Arsenic in Fronds of Pteris vittata
title_fullStr	Evidence for exocellular Arsenic in Fronds of Pteris vittata
title_full_unstemmed	Evidence for exocellular Arsenic in Fronds of Pteris vittata
title_sort	evidence for exocellular arsenic in fronds of pteris vittata
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/07ce908e7339469db4c55fcbbe780781
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Evidence for exocellular Arsenic in Fronds of Pteris vittata

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