An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence

Abstract The overall light energy to biomass conversion efficiency of plant photosynthesis is generally regarded as low. Forward genetic screens in Arabidopsis have yielded very few mutants with substantially enhanced photochemistry. Here, we report the isolation of a novel Arabidopsis mutant with a...

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Autores principales:	Niels van Tol, Martijn Rolloos, Dieuwertje Augustijn, A. Alia, Huub J. de Groot, Paul J. J. Hooykaas, Bert J. van der Zaal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07d0078d068c4d11a9846fcebdf4ed31
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spelling	oai:doaj.org-article:07d0078d068c4d11a9846fcebdf4ed312021-12-02T12:32:39ZAn Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence10.1038/s41598-017-03611-12045-2322https://doaj.org/article/07d0078d068c4d11a9846fcebdf4ed312017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03611-1https://doaj.org/toc/2045-2322Abstract The overall light energy to biomass conversion efficiency of plant photosynthesis is generally regarded as low. Forward genetic screens in Arabidopsis have yielded very few mutants with substantially enhanced photochemistry. Here, we report the isolation of a novel Arabidopsis mutant with a high operating efficiency of Photosystem II (φPSII) and low chlorophyll fluorescence from a library of lines harboring T-DNA constructs encoding artificial transcription factors. This mutant was named Low Chlorophyll Fluorescence 1 (LCF1). Only a single T-DNA insertion was detected in LCF1, which interrupted the expression of the full length mRNA of the gene At4g36280 (MORC2). We demonstrate that the high φPSII and low levels of chlorophyll fluorescence were due to a decrease in PSII:PSI ratio. Although LCF1 plants had decreased rosette surface area and biomass under normal growth conditions, they contained more starch per gram fresh weight. The growth defect of LCF1 was alleviated by low light and short day conditions, and growth could even be enhanced after a period of dark-induced senescence, showing that the plant can utilize its excess photosynthetic conversion capacity as a resource when needed.Niels van TolMartijn RolloosDieuwertje AugustijnA. AliaHuub J. de GrootPaul J. J. HooykaasBert J. van der ZaalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Niels van Tol Martijn Rolloos Dieuwertje Augustijn A. Alia Huub J. de Groot Paul J. J. Hooykaas Bert J. van der Zaal An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence
description	Abstract The overall light energy to biomass conversion efficiency of plant photosynthesis is generally regarded as low. Forward genetic screens in Arabidopsis have yielded very few mutants with substantially enhanced photochemistry. Here, we report the isolation of a novel Arabidopsis mutant with a high operating efficiency of Photosystem II (φPSII) and low chlorophyll fluorescence from a library of lines harboring T-DNA constructs encoding artificial transcription factors. This mutant was named Low Chlorophyll Fluorescence 1 (LCF1). Only a single T-DNA insertion was detected in LCF1, which interrupted the expression of the full length mRNA of the gene At4g36280 (MORC2). We demonstrate that the high φPSII and low levels of chlorophyll fluorescence were due to a decrease in PSII:PSI ratio. Although LCF1 plants had decreased rosette surface area and biomass under normal growth conditions, they contained more starch per gram fresh weight. The growth defect of LCF1 was alleviated by low light and short day conditions, and growth could even be enhanced after a period of dark-induced senescence, showing that the plant can utilize its excess photosynthetic conversion capacity as a resource when needed.
format	article
author	Niels van Tol Martijn Rolloos Dieuwertje Augustijn A. Alia Huub J. de Groot Paul J. J. Hooykaas Bert J. van der Zaal
author_facet	Niels van Tol Martijn Rolloos Dieuwertje Augustijn A. Alia Huub J. de Groot Paul J. J. Hooykaas Bert J. van der Zaal
author_sort	Niels van Tol
title	An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence
title_short	An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence
title_full	An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence
title_fullStr	An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence
title_full_unstemmed	An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence
title_sort	arabidopsis mutant with high operating efficiency of photosystem ii and low chlorophyll fluorescence
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/07d0078d068c4d11a9846fcebdf4ed31
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An Arabidopsis mutant with high operating efficiency of Photosystem II and low chlorophyll fluorescence

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