CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.

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Autores principales:	Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge, Jan Michiels
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/07d39f442b264873bcd3c1d920a2d3d7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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spelling	oai:doaj.org-article:07d39f442b264873bcd3c1d920a2d3d72021-12-02T16:49:32ZCRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing10.1038/s41467-018-04651-52041-1723https://doaj.org/article/07d39f442b264873bcd3c1d920a2d3d72018-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04651-5https://doaj.org/toc/2041-1723Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.Toon SwingsDavid C. MarcianoBenu AtriRachel E. BossermanChen WangMarlies LeysenCamille BonteThomas SchalckIan FureyBram Van den BerghNatalie VerstraetenPeter J. ChristieChristophe HermanOlivier LichtargeJan MichielsNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Toon Swings David C. Marciano Benu Atri Rachel E. Bosserman Chen Wang Marlies Leysen Camille Bonte Thomas Schalck Ian Furey Bram Van den Bergh Natalie Verstraeten Peter J. Christie Christophe Herman Olivier Lichtarge Jan Michiels CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
description	Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.
format	article
author	Toon Swings David C. Marciano Benu Atri Rachel E. Bosserman Chen Wang Marlies Leysen Camille Bonte Thomas Schalck Ian Furey Bram Van den Bergh Natalie Verstraeten Peter J. Christie Christophe Herman Olivier Lichtarge Jan Michiels
author_facet	Toon Swings David C. Marciano Benu Atri Rachel E. Bosserman Chen Wang Marlies Leysen Camille Bonte Thomas Schalck Ian Furey Bram Van den Bergh Natalie Verstraeten Peter J. Christie Christophe Herman Olivier Lichtarge Jan Michiels
author_sort	Toon Swings
title	CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_short	CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_full	CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_fullStr	CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_full_unstemmed	CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing
title_sort	crispr-frt targets shared sites in a knock-out collection for off-the-shelf genome editing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/07d39f442b264873bcd3c1d920a2d3d7
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CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

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