CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.

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Bibliographic Details
Main Authors: Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge, Jan Michiels
Format: article
Language:EN
Published: Nature Portfolio 2018
Subjects:
Science
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Online Access:https://doaj.org/article/07d39f442b264873bcd3c1d920a2d3d7
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