Human herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment.
<h4>Background</h4>Human herpesvirus-8 (HHV-8) is the etiological agent of Kaposi's sarcoma (KS) and of some lymphoproliferative disorders of B cells. Most malignancies develop after long-lasting viral dormancy, and a preventing role for both humoral and cellular immune control is s...
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oai:doaj.org-article:07d5416e9cf6493f8714c9577bdb59cc2021-11-18T07:36:28ZHuman herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment.1932-620310.1371/journal.pone.0015029https://doaj.org/article/07d5416e9cf6493f8714c9577bdb59cc2010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21124778/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Human herpesvirus-8 (HHV-8) is the etiological agent of Kaposi's sarcoma (KS) and of some lymphoproliferative disorders of B cells. Most malignancies develop after long-lasting viral dormancy, and a preventing role for both humoral and cellular immune control is suggested by the high frequency of these pathologies in immunosuppressed patients. B cells, macrophages and dendritic cells of peripheral lymphoid organs and blood represent the major reservoir of HHV-8. Due to the dual role of B cells in HHV-8 infection, both as virus reservoir and as agents of humoral immune control, we analyzed the subset distribution and the functional state of peripheral blood B cells in HHV-8-infected individuals with and without cKS.<h4>Methodology/principal findings</h4>Circulating B cells and their subsets were analyzed by 6-color flow cytometry in the following groups: 1- patients HHV-8 positive with classic KS (cKS) (n = 47); 2- subjects HHV-8 positive and cKS negative (HSP) (n = 10); 3- healthy controls, HHV-8 negative and cKS negative (HC) (n = 43). The number of B cells belonging to the preimmune/natural effector compartment, including transitional, pre-naïve, naïve and MZ-like subsets, was significantly higher among HHV-8 positive subjects, with or without cKS, while was comparable to healthy controls in the antigen-experienced T-cell dependent compartment. The increased number of preimmune/natural effector B cells was associated with increased resistance to spontaneous apoptosis, while it did not correlate with HHV-8 viral load.<h4>Conclusions/significance</h4>Our results indicate that long-lasting HHV-8 infection promotes an imbalance in peripheral B cell subsets, perturbing the equilibrium between earlier and later steps of maturation and activation processes. This observation may broaden our understanding of the complex interplay between viral and immune factors leading HHV-8-infected individuals to develop HHV-8-associated malignancies.Silvia Della BellaAdriano TaddeoElena ColomboLucia BrambillaMonica BellinviaFabrizio PregliascoMonica CappellettiMaria Luisa CalabròMaria Luisa VillaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 11, p e15029 (2010) |
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Medicine R Science Q Silvia Della Bella Adriano Taddeo Elena Colombo Lucia Brambilla Monica Bellinvia Fabrizio Pregliasco Monica Cappelletti Maria Luisa Calabrò Maria Luisa Villa Human herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment. |
| description |
<h4>Background</h4>Human herpesvirus-8 (HHV-8) is the etiological agent of Kaposi's sarcoma (KS) and of some lymphoproliferative disorders of B cells. Most malignancies develop after long-lasting viral dormancy, and a preventing role for both humoral and cellular immune control is suggested by the high frequency of these pathologies in immunosuppressed patients. B cells, macrophages and dendritic cells of peripheral lymphoid organs and blood represent the major reservoir of HHV-8. Due to the dual role of B cells in HHV-8 infection, both as virus reservoir and as agents of humoral immune control, we analyzed the subset distribution and the functional state of peripheral blood B cells in HHV-8-infected individuals with and without cKS.<h4>Methodology/principal findings</h4>Circulating B cells and their subsets were analyzed by 6-color flow cytometry in the following groups: 1- patients HHV-8 positive with classic KS (cKS) (n = 47); 2- subjects HHV-8 positive and cKS negative (HSP) (n = 10); 3- healthy controls, HHV-8 negative and cKS negative (HC) (n = 43). The number of B cells belonging to the preimmune/natural effector compartment, including transitional, pre-naïve, naïve and MZ-like subsets, was significantly higher among HHV-8 positive subjects, with or without cKS, while was comparable to healthy controls in the antigen-experienced T-cell dependent compartment. The increased number of preimmune/natural effector B cells was associated with increased resistance to spontaneous apoptosis, while it did not correlate with HHV-8 viral load.<h4>Conclusions/significance</h4>Our results indicate that long-lasting HHV-8 infection promotes an imbalance in peripheral B cell subsets, perturbing the equilibrium between earlier and later steps of maturation and activation processes. This observation may broaden our understanding of the complex interplay between viral and immune factors leading HHV-8-infected individuals to develop HHV-8-associated malignancies. |
| format |
article |
| author |
Silvia Della Bella Adriano Taddeo Elena Colombo Lucia Brambilla Monica Bellinvia Fabrizio Pregliasco Monica Cappelletti Maria Luisa Calabrò Maria Luisa Villa |
| author_facet |
Silvia Della Bella Adriano Taddeo Elena Colombo Lucia Brambilla Monica Bellinvia Fabrizio Pregliasco Monica Cappelletti Maria Luisa Calabrò Maria Luisa Villa |
| author_sort |
Silvia Della Bella |
| title |
Human herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment. |
| title_short |
Human herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment. |
| title_full |
Human herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment. |
| title_fullStr |
Human herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment. |
| title_full_unstemmed |
Human herpesvirus-8 infection leads to expansion of the preimmune/natural effector B cell compartment. |
| title_sort |
human herpesvirus-8 infection leads to expansion of the preimmune/natural effector b cell compartment. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/07d5416e9cf6493f8714c9577bdb59cc |
| work_keys_str_mv |
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