The generation and use of recombinant extracellular vesicles as biological reference material
There is no universal reference material to develop extracellular vesicle (EV) separation methods and carry out calibration and normalization. Here the authors use HIV-derived gag proteins to assemble recombinant fluorescent EV as a trackable reference material resembling the physical and biochemica...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2019
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/07dfa2e581d14bf79f66bb78fe9a3cd4 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:07dfa2e581d14bf79f66bb78fe9a3cd4 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:07dfa2e581d14bf79f66bb78fe9a3cd42021-12-02T15:36:21ZThe generation and use of recombinant extracellular vesicles as biological reference material10.1038/s41467-019-11182-02041-1723https://doaj.org/article/07dfa2e581d14bf79f66bb78fe9a3cd42019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11182-0https://doaj.org/toc/2041-1723There is no universal reference material to develop extracellular vesicle (EV) separation methods and carry out calibration and normalization. Here the authors use HIV-derived gag proteins to assemble recombinant fluorescent EV as a trackable reference material resembling the physical and biochemical properties of sample EV.Edward GeeurickxJoeri TulkensBert DhondtJan Van DeunLien LippensGlenn VergauwenElisa HeyrmanDelphine De SutterKris GevaertFrancis ImpensIlkka MiinalainenPieter-Jan Van BockstalThomas De BeerMarca H. M. WaubenEsther N. M. Nolte-‘t-HoenKatarzyna BlochJohannes V. SwinnenEdwin van der PolRienk NieuwlandGeert BraemsNico CallewaertPieter MestdaghJo VandesompeleHannelore DenysSven EyckermanOlivier De WeverAn HendrixNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Science Q |
| spellingShingle |
Science Q Edward Geeurickx Joeri Tulkens Bert Dhondt Jan Van Deun Lien Lippens Glenn Vergauwen Elisa Heyrman Delphine De Sutter Kris Gevaert Francis Impens Ilkka Miinalainen Pieter-Jan Van Bockstal Thomas De Beer Marca H. M. Wauben Esther N. M. Nolte-‘t-Hoen Katarzyna Bloch Johannes V. Swinnen Edwin van der Pol Rienk Nieuwland Geert Braems Nico Callewaert Pieter Mestdagh Jo Vandesompele Hannelore Denys Sven Eyckerman Olivier De Wever An Hendrix The generation and use of recombinant extracellular vesicles as biological reference material |
| description |
There is no universal reference material to develop extracellular vesicle (EV) separation methods and carry out calibration and normalization. Here the authors use HIV-derived gag proteins to assemble recombinant fluorescent EV as a trackable reference material resembling the physical and biochemical properties of sample EV. |
| format |
article |
| author |
Edward Geeurickx Joeri Tulkens Bert Dhondt Jan Van Deun Lien Lippens Glenn Vergauwen Elisa Heyrman Delphine De Sutter Kris Gevaert Francis Impens Ilkka Miinalainen Pieter-Jan Van Bockstal Thomas De Beer Marca H. M. Wauben Esther N. M. Nolte-‘t-Hoen Katarzyna Bloch Johannes V. Swinnen Edwin van der Pol Rienk Nieuwland Geert Braems Nico Callewaert Pieter Mestdagh Jo Vandesompele Hannelore Denys Sven Eyckerman Olivier De Wever An Hendrix |
| author_facet |
Edward Geeurickx Joeri Tulkens Bert Dhondt Jan Van Deun Lien Lippens Glenn Vergauwen Elisa Heyrman Delphine De Sutter Kris Gevaert Francis Impens Ilkka Miinalainen Pieter-Jan Van Bockstal Thomas De Beer Marca H. M. Wauben Esther N. M. Nolte-‘t-Hoen Katarzyna Bloch Johannes V. Swinnen Edwin van der Pol Rienk Nieuwland Geert Braems Nico Callewaert Pieter Mestdagh Jo Vandesompele Hannelore Denys Sven Eyckerman Olivier De Wever An Hendrix |
| author_sort |
Edward Geeurickx |
| title |
The generation and use of recombinant extracellular vesicles as biological reference material |
| title_short |
The generation and use of recombinant extracellular vesicles as biological reference material |
| title_full |
The generation and use of recombinant extracellular vesicles as biological reference material |
| title_fullStr |
The generation and use of recombinant extracellular vesicles as biological reference material |
| title_full_unstemmed |
The generation and use of recombinant extracellular vesicles as biological reference material |
| title_sort |
generation and use of recombinant extracellular vesicles as biological reference material |
| publisher |
Nature Portfolio |
| publishDate |
2019 |
| url |
https://doaj.org/article/07dfa2e581d14bf79f66bb78fe9a3cd4 |
| work_keys_str_mv |
AT edwardgeeurickx thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT joeritulkens thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT bertdhondt thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT janvandeun thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT lienlippens thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT glennvergauwen thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT elisaheyrman thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT delphinedesutter thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT krisgevaert thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT francisimpens thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT ilkkamiinalainen thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT pieterjanvanbockstal thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT thomasdebeer thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT marcahmwauben thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT esthernmnoltethoen thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT katarzynabloch thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT johannesvswinnen thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT edwinvanderpol thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT rienknieuwland thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT geertbraems thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT nicocallewaert thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT pietermestdagh thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT jovandesompele thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT hanneloredenys thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT sveneyckerman thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT olivierdewever thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT anhendrix thegenerationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT edwardgeeurickx generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT joeritulkens generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT bertdhondt generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT janvandeun generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT lienlippens generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT glennvergauwen generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT elisaheyrman generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT delphinedesutter generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT krisgevaert generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT francisimpens generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT ilkkamiinalainen generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT pieterjanvanbockstal generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT thomasdebeer generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT marcahmwauben generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT esthernmnoltethoen generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT katarzynabloch generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT johannesvswinnen generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT edwinvanderpol generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT rienknieuwland generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT geertbraems generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT nicocallewaert generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT pietermestdagh generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT jovandesompele generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT hanneloredenys generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT sveneyckerman generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT olivierdewever generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial AT anhendrix generationanduseofrecombinantextracellularvesiclesasbiologicalreferencematerial |
| _version_ |
1718386272998785024 |