Diversity of Potentially Pathogenic Escherichia coli O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions

In 2011, an outbreak of hemorrhagic colitis and hemolytic uremic syndrome (HUS) was reported in Europe that was related to a hybrid STEAEC of Escherichia coli (E. coli) O104:H4 strain. The current study aimed to analyze strains of E. coli O104...

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Autores principales:	Armando Navarro, Claudia van der Ploeg, Ariel Rogé, Delia Licona-Moreno, Gabriela Delgado, Rosario Morales-Espinosa, Alejandro Cravioto, Carlos Eslava
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Escherichia coli</i> O104:H4 serotypes virulence genes STEAEC DEC pathotypes Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/07e0b4418c1e46359be2686ef8923fb3
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spelling	oai:doaj.org-article:07e0b4418c1e46359be2686ef8923fb32021-11-25T18:24:23ZDiversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions10.3390/microorganisms91122272076-2607https://doaj.org/article/07e0b4418c1e46359be2686ef8923fb32021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2227https://doaj.org/toc/2076-2607In 2011, an outbreak of hemorrhagic colitis and hemolytic uremic syndrome (HUS) was reported in Europe that was related to a hybrid STEAEC of <i>Escherichia coli</i> (<i>E. coli</i>) O104:H4 strain. The current study aimed to analyze strains of <i>E. coli</i> O104 and O9 isolated before 2011. The study included 47 strains isolated from children with and without diarrhea between 1986 and 2009 from different geographic regions, as well as seven reference strains. Serotyping was carried out on 188 anti-O and 53 anti-H sera. PCR was used to identify DEC genes and phylogenetic groups. Resistance profiles to antimicrobials were determined by diffusion in agar, while PFGE was used to analyze genomic similarity. Five serotypes of <i>E. coli</i> O104 and nine of O9 were identified, as well as an antigenic cross-reaction with one anti-<i>E. coli</i> O9 serum. <i>E. coli</i> O104 and O9 presented diarrheagenic <i>E</i>. <i>coli</i> (DEC) genes in different combinations and were located in commensal phylogenetic groups with different antimicrobial resistance. PFGE showed that O104:H4 and O9:(H4, NM) strains from SSI, Bangladesh and México belong to a diverse group located in the same subgroup. <i>E. coli</i> O104 and O9 were classified as commensal strains containing DEC genes. The groups were genetically diverse with pathogenic potential making continued epidemiologic surveillance important.Armando NavarroClaudia van der PloegAriel RogéDelia Licona-MorenoGabriela DelgadoRosario Morales-EspinosaAlejandro CraviotoCarlos EslavaMDPI AGarticle<i>Escherichia coli</i> O104:H4serotypesvirulence genesSTEAECDEC pathotypesBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2227, p 2227 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Escherichia coli</i> O104:H4 serotypes virulence genes STEAEC DEC pathotypes Biology (General) QH301-705.5
spellingShingle	<i>Escherichia coli</i> O104:H4 serotypes virulence genes STEAEC DEC pathotypes Biology (General) QH301-705.5 Armando Navarro Claudia van der Ploeg Ariel Rogé Delia Licona-Moreno Gabriela Delgado Rosario Morales-Espinosa Alejandro Cravioto Carlos Eslava Diversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions
description	In 2011, an outbreak of hemorrhagic colitis and hemolytic uremic syndrome (HUS) was reported in Europe that was related to a hybrid STEAEC of <i>Escherichia coli</i> (<i>E. coli</i>) O104:H4 strain. The current study aimed to analyze strains of <i>E. coli</i> O104 and O9 isolated before 2011. The study included 47 strains isolated from children with and without diarrhea between 1986 and 2009 from different geographic regions, as well as seven reference strains. Serotyping was carried out on 188 anti-O and 53 anti-H sera. PCR was used to identify DEC genes and phylogenetic groups. Resistance profiles to antimicrobials were determined by diffusion in agar, while PFGE was used to analyze genomic similarity. Five serotypes of <i>E. coli</i> O104 and nine of O9 were identified, as well as an antigenic cross-reaction with one anti-<i>E. coli</i> O9 serum. <i>E. coli</i> O104 and O9 presented diarrheagenic <i>E</i>. <i>coli</i> (DEC) genes in different combinations and were located in commensal phylogenetic groups with different antimicrobial resistance. PFGE showed that O104:H4 and O9:(H4, NM) strains from SSI, Bangladesh and México belong to a diverse group located in the same subgroup. <i>E. coli</i> O104 and O9 were classified as commensal strains containing DEC genes. The groups were genetically diverse with pathogenic potential making continued epidemiologic surveillance important.
format	article
author	Armando Navarro Claudia van der Ploeg Ariel Rogé Delia Licona-Moreno Gabriela Delgado Rosario Morales-Espinosa Alejandro Cravioto Carlos Eslava
author_facet	Armando Navarro Claudia van der Ploeg Ariel Rogé Delia Licona-Moreno Gabriela Delgado Rosario Morales-Espinosa Alejandro Cravioto Carlos Eslava
author_sort	Armando Navarro
title	Diversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions
title_short	Diversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions
title_full	Diversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions
title_fullStr	Diversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions
title_full_unstemmed	Diversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions
title_sort	diversity of potentially pathogenic <i>escherichia coli</i> o104 and o9 serogroups isolated before 2011 from fecal samples from children from different geographic regions
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/07e0b4418c1e46359be2686ef8923fb3
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Diversity of Potentially Pathogenic <i>Escherichia coli</i> O104 and O9 Serogroups Isolated before 2011 from Fecal Samples from Children from Different Geographic Regions

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