Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells

Abstract Mitochondrial health plays a crucial role in human brain development and diseases. However, the evaluation of mitochondrial health in the brain is not incorporated into clinical practice due to ethical and logistical concerns. As a result, the development of targeted mitochondrial therapeut...

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Autores principales:	Angela Duong, Alesya Evstratova, Adam Sivitilli, J. Javier Hernandez, Jessica Gosio, Azizia Wahedi, Neal Sondheimer, Jeff L. Wrana, Jean-Martin Beaulieu, Liliana Attisano, Ana C. Andreazza
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07e67ce070a84572b832c40c4b1c99f0
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spelling	oai:doaj.org-article:07e67ce070a84572b832c40c4b1c99f02021-12-02T11:35:41ZCharacterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells10.1038/s41598-021-84071-62045-2322https://doaj.org/article/07e67ce070a84572b832c40c4b1c99f02021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84071-6https://doaj.org/toc/2045-2322Abstract Mitochondrial health plays a crucial role in human brain development and diseases. However, the evaluation of mitochondrial health in the brain is not incorporated into clinical practice due to ethical and logistical concerns. As a result, the development of targeted mitochondrial therapeutics remains a significant challenge due to the lack of appropriate patient-derived brain tissues. To address these unmet needs, we developed cerebral organoids (COs) from induced pluripotent stem cells (iPSCs) derived from human peripheral blood mononuclear cells (PBMCs) and monitored mitochondrial health from the primary, reprogrammed and differentiated stages. Our results show preserved mitochondrial genetics, function and treatment responses across PBMCs to iPSCs to COs, and measurable neuronal activity in the COs. We expect our approach will serve as a model for more widespread evaluation of mitochondrial health relevant to a wide range of human diseases using readily accessible patient peripheral (PBMCs) and stem-cell derived brain tissue samples.Angela DuongAlesya EvstratovaAdam SivitilliJ. Javier HernandezJessica GosioAzizia WahediNeal SondheimerJeff L. WranaJean-Martin BeaulieuLiliana AttisanoAna C. AndreazzaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Angela Duong Alesya Evstratova Adam Sivitilli J. Javier Hernandez Jessica Gosio Azizia Wahedi Neal Sondheimer Jeff L. Wrana Jean-Martin Beaulieu Liliana Attisano Ana C. Andreazza Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells
description	Abstract Mitochondrial health plays a crucial role in human brain development and diseases. However, the evaluation of mitochondrial health in the brain is not incorporated into clinical practice due to ethical and logistical concerns. As a result, the development of targeted mitochondrial therapeutics remains a significant challenge due to the lack of appropriate patient-derived brain tissues. To address these unmet needs, we developed cerebral organoids (COs) from induced pluripotent stem cells (iPSCs) derived from human peripheral blood mononuclear cells (PBMCs) and monitored mitochondrial health from the primary, reprogrammed and differentiated stages. Our results show preserved mitochondrial genetics, function and treatment responses across PBMCs to iPSCs to COs, and measurable neuronal activity in the COs. We expect our approach will serve as a model for more widespread evaluation of mitochondrial health relevant to a wide range of human diseases using readily accessible patient peripheral (PBMCs) and stem-cell derived brain tissue samples.
format	article
author	Angela Duong Alesya Evstratova Adam Sivitilli J. Javier Hernandez Jessica Gosio Azizia Wahedi Neal Sondheimer Jeff L. Wrana Jean-Martin Beaulieu Liliana Attisano Ana C. Andreazza
author_facet	Angela Duong Alesya Evstratova Adam Sivitilli J. Javier Hernandez Jessica Gosio Azizia Wahedi Neal Sondheimer Jeff L. Wrana Jean-Martin Beaulieu Liliana Attisano Ana C. Andreazza
author_sort	Angela Duong
title	Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells
title_short	Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells
title_full	Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells
title_fullStr	Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells
title_full_unstemmed	Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells
title_sort	characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/07e67ce070a84572b832c40c4b1c99f0
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Characterization of mitochondrial health from human peripheral blood mononuclear cells to cerebral organoids derived from induced pluripotent stem cells

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