Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Delivery of Cas9 ribonucleoprotein complexes can be done via microinjection into eggs, though this is a technically challenging procedure. Here the authors demonstrate ReMOT Control, delivery of RNPs to the mosquito germline by tagging with peptide derived from yolk protein 1.

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Autores principales:	Duverney Chaverra-Rodriguez, Vanessa M. Macias, Grant L. Hughes, Sujit Pujhari, Yasutsugu Suzuki, David R. Peterson, Donghun Kim, Sage McKeand, Jason L. Rasgon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/07ec832a764747dfbd6c8d3b65021968
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spelling	oai:doaj.org-article:07ec832a764747dfbd6c8d3b650219682021-12-02T16:50:05ZTargeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing10.1038/s41467-018-05425-92041-1723https://doaj.org/article/07ec832a764747dfbd6c8d3b650219682018-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-05425-9https://doaj.org/toc/2041-1723Delivery of Cas9 ribonucleoprotein complexes can be done via microinjection into eggs, though this is a technically challenging procedure. Here the authors demonstrate ReMOT Control, delivery of RNPs to the mosquito germline by tagging with peptide derived from yolk protein 1.Duverney Chaverra-RodriguezVanessa M. MaciasGrant L. HughesSujit PujhariYasutsugu SuzukiDavid R. PetersonDonghun KimSage McKeandJason L. RasgonNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Duverney Chaverra-Rodriguez Vanessa M. Macias Grant L. Hughes Sujit Pujhari Yasutsugu Suzuki David R. Peterson Donghun Kim Sage McKeand Jason L. Rasgon Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
description	Delivery of Cas9 ribonucleoprotein complexes can be done via microinjection into eggs, though this is a technically challenging procedure. Here the authors demonstrate ReMOT Control, delivery of RNPs to the mosquito germline by tagging with peptide derived from yolk protein 1.
format	article
author	Duverney Chaverra-Rodriguez Vanessa M. Macias Grant L. Hughes Sujit Pujhari Yasutsugu Suzuki David R. Peterson Donghun Kim Sage McKeand Jason L. Rasgon
author_facet	Duverney Chaverra-Rodriguez Vanessa M. Macias Grant L. Hughes Sujit Pujhari Yasutsugu Suzuki David R. Peterson Donghun Kim Sage McKeand Jason L. Rasgon
author_sort	Duverney Chaverra-Rodriguez
title	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_short	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_full	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_fullStr	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_full_unstemmed	Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
title_sort	targeted delivery of crispr-cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/07ec832a764747dfbd6c8d3b65021968
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Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

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