Proteomic profiling of the endogenous peptides of MRSA and MSSA

Staphylococcus aureus is a Gram-positive bacterium that can cause diverse skin and soft tissue infections. Methicillin-resistant Staphylococcus aureus (MRSA) can cause more severe infections than methicillin-susceptible Staphylococcus aureus (MSSA). Nevertheless, the physiological and metabolic regu...

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Autores principales:	Haixia Tu, Fei Xu, Yiwei Cheng, Qianglong Pan, Xiao Cai, Shouxing Wang, Shuting Ge, Min Cao, Dongming Su, Yan Li
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Staphylococcus aureus Differential endogenous peptidome Protein Mass spectrometry MRSA Medicine R
Acceso en línea:	https://doaj.org/article/07f3801a0d74408ab1bc7aa18c937a94
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spelling	oai:doaj.org-article:07f3801a0d74408ab1bc7aa18c937a942021-11-26T15:05:35ZProteomic profiling of the endogenous peptides of MRSA and MSSA10.7717/peerj.125082167-8359https://doaj.org/article/07f3801a0d74408ab1bc7aa18c937a942021-11-01T00:00:00Zhttps://peerj.com/articles/12508.pdfhttps://peerj.com/articles/12508/https://doaj.org/toc/2167-8359Staphylococcus aureus is a Gram-positive bacterium that can cause diverse skin and soft tissue infections. Methicillin-resistant Staphylococcus aureus (MRSA) can cause more severe infections than methicillin-susceptible Staphylococcus aureus (MSSA). Nevertheless, the physiological and metabolic regulation of MSSA and MRSA has not been well studied. In light of the increased interest in endogenous peptides and recognition of the important roles that they play, we studied the endogenous peptidome of MSSA and MRSA. We identified 1,065 endogenous peptides, among which 435 were differentially expressed (DE), with 292 MSSA-abundant endogenous peptides and 35 MRSA-abundant endogenous peptides. MSSA-abundant endogenous peptides have significantly enriched “VXXXK” motif of at the C-terminus. MSSA-abundant endogenous peptides are involved in penicillin-binding and immune responses, whereas MRSA-abundant endogenous peptides are associated with antibiotic resistance and increased toxicity. Our characterization of the peptidome of MSSA and MRSA provides a rich resource for future studies to explore the functional regulation of drug resistance in S. aureus and may also help elucidate the mechanisms of its pathogenicity and the development of treatments.Haixia TuFei XuYiwei ChengQianglong PanXiao CaiShouxing WangShuting GeMin CaoDongming SuYan LiPeerJ Inc.articleStaphylococcus aureusDifferential endogenous peptidomeProteinMass spectrometryMRSAMedicineRENPeerJ, Vol 9, p e12508 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Staphylococcus aureus Differential endogenous peptidome Protein Mass spectrometry MRSA Medicine R
spellingShingle	Staphylococcus aureus Differential endogenous peptidome Protein Mass spectrometry MRSA Medicine R Haixia Tu Fei Xu Yiwei Cheng Qianglong Pan Xiao Cai Shouxing Wang Shuting Ge Min Cao Dongming Su Yan Li Proteomic profiling of the endogenous peptides of MRSA and MSSA
description	Staphylococcus aureus is a Gram-positive bacterium that can cause diverse skin and soft tissue infections. Methicillin-resistant Staphylococcus aureus (MRSA) can cause more severe infections than methicillin-susceptible Staphylococcus aureus (MSSA). Nevertheless, the physiological and metabolic regulation of MSSA and MRSA has not been well studied. In light of the increased interest in endogenous peptides and recognition of the important roles that they play, we studied the endogenous peptidome of MSSA and MRSA. We identified 1,065 endogenous peptides, among which 435 were differentially expressed (DE), with 292 MSSA-abundant endogenous peptides and 35 MRSA-abundant endogenous peptides. MSSA-abundant endogenous peptides have significantly enriched “VXXXK” motif of at the C-terminus. MSSA-abundant endogenous peptides are involved in penicillin-binding and immune responses, whereas MRSA-abundant endogenous peptides are associated with antibiotic resistance and increased toxicity. Our characterization of the peptidome of MSSA and MRSA provides a rich resource for future studies to explore the functional regulation of drug resistance in S. aureus and may also help elucidate the mechanisms of its pathogenicity and the development of treatments.
format	article
author	Haixia Tu Fei Xu Yiwei Cheng Qianglong Pan Xiao Cai Shouxing Wang Shuting Ge Min Cao Dongming Su Yan Li
author_facet	Haixia Tu Fei Xu Yiwei Cheng Qianglong Pan Xiao Cai Shouxing Wang Shuting Ge Min Cao Dongming Su Yan Li
author_sort	Haixia Tu
title	Proteomic profiling of the endogenous peptides of MRSA and MSSA
title_short	Proteomic profiling of the endogenous peptides of MRSA and MSSA
title_full	Proteomic profiling of the endogenous peptides of MRSA and MSSA
title_fullStr	Proteomic profiling of the endogenous peptides of MRSA and MSSA
title_full_unstemmed	Proteomic profiling of the endogenous peptides of MRSA and MSSA
title_sort	proteomic profiling of the endogenous peptides of mrsa and mssa
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/07f3801a0d74408ab1bc7aa18c937a94
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Proteomic profiling of the endogenous peptides of MRSA and MSSA

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