Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon

Abstract Marine invertebrates associate with diverse microorganisms. Microorganisms even inhabit coelomic fluid (CF), namely, the fluid filling the main body cavity of echinoderms. The CF microbiota potentially impacts host health and disease. Here, we analysed the CF microbiota in two common coasta...

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Autores principales:	Satoshi Nakagawa, Hikari Saito, Akihiro Tame, Miho Hirai, Hideyuki Yamaguchi, Takashi Sunata, Masanori Aida, Hisashi Muto, Shigeki Sawayama, Yoshihiro Takaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07f6cf7d183040689d23ba67b17fe92b
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spelling	oai:doaj.org-article:07f6cf7d183040689d23ba67b17fe92b2021-12-02T11:53:03ZMicrobiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon10.1038/s41598-017-09355-22045-2322https://doaj.org/article/07f6cf7d183040689d23ba67b17fe92b2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09355-2https://doaj.org/toc/2045-2322Abstract Marine invertebrates associate with diverse microorganisms. Microorganisms even inhabit coelomic fluid (CF), namely, the fluid filling the main body cavity of echinoderms. The CF microbiota potentially impacts host health and disease. Here, we analysed the CF microbiota in two common coastal starfish species, Patiria pectinifera and Asterias amurensis. Although microbial community structures were highly variable among individual starfish, those of P. pectinifera were compositionally similar to those in the surrounding seawater. By contrast, many A. amurensis individuals harboured unique microbes in the CF, which was dominated by the unclassified Thiotrichales or previously unknown Helicobacter-related taxon. In some individuals, the Helicobacter-related taxon was the most abundant genus-level taxon, accounting for up to 97.3% of reads obtained from the CF microbial community. Fluorescence in situ hybridization using a Helicobacter-related-taxon-specific probe suggested that probe-reactive cells in A. amurensis were spiral-shaped, morphologically similar to known Helicobacter species. Electron microscopy revealed that the spiral cells had a prosthecate-like polar appendage that has never been reported in Helicobacter species. Although culture of Helicobacter-related taxon was unsuccessful, this is the first report of the dominance of a Helicobacter-related taxon in invertebrates and non-digestive organs, reshaping our knowledge of the phylogeography of Helicobacter-related taxa.Satoshi NakagawaHikari SaitoAkihiro TameMiho HiraiHideyuki YamaguchiTakashi SunataMasanori AidaHisashi MutoShigeki SawayamaYoshihiro TakakiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Satoshi Nakagawa Hikari Saito Akihiro Tame Miho Hirai Hideyuki Yamaguchi Takashi Sunata Masanori Aida Hisashi Muto Shigeki Sawayama Yoshihiro Takaki Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon
description	Abstract Marine invertebrates associate with diverse microorganisms. Microorganisms even inhabit coelomic fluid (CF), namely, the fluid filling the main body cavity of echinoderms. The CF microbiota potentially impacts host health and disease. Here, we analysed the CF microbiota in two common coastal starfish species, Patiria pectinifera and Asterias amurensis. Although microbial community structures were highly variable among individual starfish, those of P. pectinifera were compositionally similar to those in the surrounding seawater. By contrast, many A. amurensis individuals harboured unique microbes in the CF, which was dominated by the unclassified Thiotrichales or previously unknown Helicobacter-related taxon. In some individuals, the Helicobacter-related taxon was the most abundant genus-level taxon, accounting for up to 97.3% of reads obtained from the CF microbial community. Fluorescence in situ hybridization using a Helicobacter-related-taxon-specific probe suggested that probe-reactive cells in A. amurensis were spiral-shaped, morphologically similar to known Helicobacter species. Electron microscopy revealed that the spiral cells had a prosthecate-like polar appendage that has never been reported in Helicobacter species. Although culture of Helicobacter-related taxon was unsuccessful, this is the first report of the dominance of a Helicobacter-related taxon in invertebrates and non-digestive organs, reshaping our knowledge of the phylogeography of Helicobacter-related taxa.
format	article
author	Satoshi Nakagawa Hikari Saito Akihiro Tame Miho Hirai Hideyuki Yamaguchi Takashi Sunata Masanori Aida Hisashi Muto Shigeki Sawayama Yoshihiro Takaki
author_facet	Satoshi Nakagawa Hikari Saito Akihiro Tame Miho Hirai Hideyuki Yamaguchi Takashi Sunata Masanori Aida Hisashi Muto Shigeki Sawayama Yoshihiro Takaki
author_sort	Satoshi Nakagawa
title	Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon
title_short	Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon
title_full	Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon
title_fullStr	Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon
title_full_unstemmed	Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon
title_sort	microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant helicobacter-related taxon
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/07f6cf7d183040689d23ba67b17fe92b
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Microbiota in the coelomic fluid of two common coastal starfish species and characterization of an abundant Helicobacter-related taxon

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