Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth

Abstract Curcumin has been reported to exhibit anti-tumorigenic activity; however, since its precise actions remain unclear, its effects are considered to be deceptive. In the present study, we confirmed the anti-tumorigenic effects of curcumin on CML-derived leukemic cells in a xenograft model and...

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Autores principales:	Yonika Arum Larasati, Noriko Yoneda-Kato, Ikuko Nakamae, Takashi Yokoyama, Edy Meiyanto, Jun-ya Kato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07f6f3b6ec8249c3a3d4c9e91b652c41
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spelling	oai:doaj.org-article:07f6f3b6ec8249c3a3d4c9e91b652c412021-12-02T15:08:27ZCurcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth10.1038/s41598-018-20179-62045-2322https://doaj.org/article/07f6f3b6ec8249c3a3d4c9e91b652c412018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-20179-6https://doaj.org/toc/2045-2322Abstract Curcumin has been reported to exhibit anti-tumorigenic activity; however, since its precise actions remain unclear, its effects are considered to be deceptive. In the present study, we confirmed the anti-tumorigenic effects of curcumin on CML-derived leukemic cells in a xenograft model and in vitro culture system. In vitro pull-down and mass analyses revealed a series of enzymes (carbonyl reductase, glutathione-S-transferase, glyoxalase, etc.) that function in a reactive oxygen species (ROS) metabolic pathway as curcumin-binding targets, the expression of which was up-regulated in human leukemia. Curcumin increased ROS levels over the threshold in leukemic cells, and the antioxidant, glutathione (GSH) and overexpression of curcumin-binding enzymes partially mitigated the up-regulation of ROS and growth inhibition caused by curcumin. These results show that curcumin specifically inhibits tumor growth by increasing ROS levels over the threshold through the miscellaneous inhibition of ROS metabolic enzymes. Curcumin has potential in therapy to regulate ROS levels in tumor cells, thereby controlling tumor growth.Yonika Arum LarasatiNoriko Yoneda-KatoIkuko NakamaeTakashi YokoyamaEdy MeiyantoJun-ya KatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yonika Arum Larasati Noriko Yoneda-Kato Ikuko Nakamae Takashi Yokoyama Edy Meiyanto Jun-ya Kato Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth
description	Abstract Curcumin has been reported to exhibit anti-tumorigenic activity; however, since its precise actions remain unclear, its effects are considered to be deceptive. In the present study, we confirmed the anti-tumorigenic effects of curcumin on CML-derived leukemic cells in a xenograft model and in vitro culture system. In vitro pull-down and mass analyses revealed a series of enzymes (carbonyl reductase, glutathione-S-transferase, glyoxalase, etc.) that function in a reactive oxygen species (ROS) metabolic pathway as curcumin-binding targets, the expression of which was up-regulated in human leukemia. Curcumin increased ROS levels over the threshold in leukemic cells, and the antioxidant, glutathione (GSH) and overexpression of curcumin-binding enzymes partially mitigated the up-regulation of ROS and growth inhibition caused by curcumin. These results show that curcumin specifically inhibits tumor growth by increasing ROS levels over the threshold through the miscellaneous inhibition of ROS metabolic enzymes. Curcumin has potential in therapy to regulate ROS levels in tumor cells, thereby controlling tumor growth.
format	article
author	Yonika Arum Larasati Noriko Yoneda-Kato Ikuko Nakamae Takashi Yokoyama Edy Meiyanto Jun-ya Kato
author_facet	Yonika Arum Larasati Noriko Yoneda-Kato Ikuko Nakamae Takashi Yokoyama Edy Meiyanto Jun-ya Kato
author_sort	Yonika Arum Larasati
title	Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth
title_short	Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth
title_full	Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth
title_fullStr	Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth
title_full_unstemmed	Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth
title_sort	curcumin targets multiple enzymes involved in the ros metabolic pathway to suppress tumor cell growth
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/07f6f3b6ec8249c3a3d4c9e91b652c41
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Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth

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