Does algae β-glucan affect the fecal bacteriome in dairy calves?

β-glucans has been reported to be associated with many health-promoting and improvements in animal performance, however, information about their effects on the bacterial community remains unknown. This study aimed to investigate how the addition of β-glucans can affect the fecal bacterial community...

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Autores principales:	Gercino Ferreira Virginio Junior, Maria Eduarda Reis, Ana Paula da Silva, Ariany Faria de Toledo, Amanda Moelemberg Cezar, Lucas William Mendes, Leandro Greco, Horácio Montenegro, Luiz Lehmann Coutinho, Carla Maris Machado Bittar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07f7ef72785948b8a4148ea67c50b9a6
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spelling	oai:doaj.org-article:07f7ef72785948b8a4148ea67c50b9a62021-12-02T20:13:53ZDoes algae β-glucan affect the fecal bacteriome in dairy calves?1932-620310.1371/journal.pone.0258069https://doaj.org/article/07f7ef72785948b8a4148ea67c50b9a62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0258069https://doaj.org/toc/1932-6203β-glucans has been reported to be associated with many health-promoting and improvements in animal performance, however, information about their effects on the bacterial community remains unknown. This study aimed to investigate how the addition of β-glucans can affect the fecal bacterial community with possible consequences on animal growth and health. For this, newborn Holstein calves (n = 14) were individually housed in tropical shelters and blocked according to sex, date, and weight at birth and randomly assigned to one of the following treatments: (1) Control: milk replacer (14% solids, 24% CP, 18.5% fat); (2) β-glucans: milk replacer supplemented with β-glucans (2 g/d). All calves were bucket fed 6 L/d of milk replacer and received water and starter concentrate ad libitum starting on d 2. To evaluate the bacteriome, fecal samples were collected at weeks 1, 2, 4, and 8. The bacterial community was assessed through sequencing of the V3-V4 region of the 16S rRNA gene on the Illumina MiSeq platform and analyzed using the DADA2 pipeline. No differences for Shannon and Chao1 indexes were observed for treatments, but both indexes increased with age (P < 0.001). There were dissimilarities in the structure of the bacterial community during the pre-weaning period (P = 0.01). In a deeper taxonomic level, Collinsella (Actinobacteriota), Prevotella (Bacteroidota), and Lactobacillus (Firmicutes) were the most abundant genera (9.84, 9.54, and 8.82% of the sequences, respectively). β-glucans promoted a higher abundance of Alloprevotella and Holdemanella, which may indicate a beneficial effect of supplementation on dairy calves. The bacterial community was highly correlated with the fecal score at weeks 1 and 2 and with starter concentrate intake at week 8. In conclusion, algae β-glucan supplementation could be beneficial to fecal bacteriome and consequently to the health and performance of dairy calves.Gercino Ferreira Virginio JuniorMaria Eduarda ReisAna Paula da SilvaAriany Faria de ToledoAmanda Moelemberg CezarLucas William MendesLeandro GrecoHorácio MontenegroLuiz Lehmann CoutinhoCarla Maris Machado BittarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0258069 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gercino Ferreira Virginio Junior Maria Eduarda Reis Ana Paula da Silva Ariany Faria de Toledo Amanda Moelemberg Cezar Lucas William Mendes Leandro Greco Horácio Montenegro Luiz Lehmann Coutinho Carla Maris Machado Bittar Does algae β-glucan affect the fecal bacteriome in dairy calves?
description	β-glucans has been reported to be associated with many health-promoting and improvements in animal performance, however, information about their effects on the bacterial community remains unknown. This study aimed to investigate how the addition of β-glucans can affect the fecal bacterial community with possible consequences on animal growth and health. For this, newborn Holstein calves (n = 14) were individually housed in tropical shelters and blocked according to sex, date, and weight at birth and randomly assigned to one of the following treatments: (1) Control: milk replacer (14% solids, 24% CP, 18.5% fat); (2) β-glucans: milk replacer supplemented with β-glucans (2 g/d). All calves were bucket fed 6 L/d of milk replacer and received water and starter concentrate ad libitum starting on d 2. To evaluate the bacteriome, fecal samples were collected at weeks 1, 2, 4, and 8. The bacterial community was assessed through sequencing of the V3-V4 region of the 16S rRNA gene on the Illumina MiSeq platform and analyzed using the DADA2 pipeline. No differences for Shannon and Chao1 indexes were observed for treatments, but both indexes increased with age (P < 0.001). There were dissimilarities in the structure of the bacterial community during the pre-weaning period (P = 0.01). In a deeper taxonomic level, Collinsella (Actinobacteriota), Prevotella (Bacteroidota), and Lactobacillus (Firmicutes) were the most abundant genera (9.84, 9.54, and 8.82% of the sequences, respectively). β-glucans promoted a higher abundance of Alloprevotella and Holdemanella, which may indicate a beneficial effect of supplementation on dairy calves. The bacterial community was highly correlated with the fecal score at weeks 1 and 2 and with starter concentrate intake at week 8. In conclusion, algae β-glucan supplementation could be beneficial to fecal bacteriome and consequently to the health and performance of dairy calves.
format	article
author	Gercino Ferreira Virginio Junior Maria Eduarda Reis Ana Paula da Silva Ariany Faria de Toledo Amanda Moelemberg Cezar Lucas William Mendes Leandro Greco Horácio Montenegro Luiz Lehmann Coutinho Carla Maris Machado Bittar
author_facet	Gercino Ferreira Virginio Junior Maria Eduarda Reis Ana Paula da Silva Ariany Faria de Toledo Amanda Moelemberg Cezar Lucas William Mendes Leandro Greco Horácio Montenegro Luiz Lehmann Coutinho Carla Maris Machado Bittar
author_sort	Gercino Ferreira Virginio Junior
title	Does algae β-glucan affect the fecal bacteriome in dairy calves?
title_short	Does algae β-glucan affect the fecal bacteriome in dairy calves?
title_full	Does algae β-glucan affect the fecal bacteriome in dairy calves?
title_fullStr	Does algae β-glucan affect the fecal bacteriome in dairy calves?
title_full_unstemmed	Does algae β-glucan affect the fecal bacteriome in dairy calves?
title_sort	does algae β-glucan affect the fecal bacteriome in dairy calves?
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/07f7ef72785948b8a4148ea67c50b9a6
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Does algae β-glucan affect the fecal bacteriome in dairy calves?

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