Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken
Cefepime is a broad-spectrum semi synthetic β-lactamase resistant fourth generation cephalosporin. Looking to potential for clinical use, pharmacokinetics of cefepime following single intravenous and intramuscular (IM) dose (100mg/kg b. wt.) in healthy and experimentally Salmonella typhimurium infe...
Guardado en:
Autor principal: | |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
South Valley University
2018
|
Materias: | |
Acceso en línea: | https://doaj.org/article/07f8918873744e4285049b42f1ced7f3 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:07f8918873744e4285049b42f1ced7f3 |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:07f8918873744e4285049b42f1ced7f32021-12-02T00:44:46ZDisposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken2535-18262535-1826https://doaj.org/article/07f8918873744e4285049b42f1ced7f32018-07-01T00:00:00Zhttps://portal.svu.edu.eg/svu-src/index.php/ijvs/article/view/10https://doaj.org/toc/2535-1826https://doaj.org/toc/2535-1826Cefepime is a broad-spectrum semi synthetic β-lactamase resistant fourth generation cephalosporin. Looking to potential for clinical use, pharmacokinetics of cefepime following single intravenous and intramuscular (IM) dose (100mg/kg b. wt.) in healthy and experimentally Salmonella typhimurium infected broiler chickens were determined. Cefepime concentration in plasma samples was determined by reverse-phase high performance liquid chromatography with mobile phase. The mobile phase was a mixture of 10mM phosphate buffer (pH 7): Methanol; 75:25 was always freshly prepared. Flow rates were 1 ml/min. UV detection was performed at 256 nm, injection volume was 20 µl. After a single intravenous injection, cefepime reached its maximum serum concentrations of 4.28 ± 0.37μg/ml in normal chickens, while in the infected chickens, the maximum serum concentration was 2.62 ± 0.72 μg/ml. Cefepime was eliminated after intravenous injection with half-life (t1/2 β) of 4.608 ± 0.145 h in normal which significantly longer than 4.19 ± 0.158 h in infected chickens. The mean residence time (MRT) was 6.51 ± 0.189h in normal vs 5.86±0.18 h in infected chickens. After IM administration the drug reached its maximum serum concentrations of 193.06 ± 2.27μg/ml at maximum time of 1.138 ± 0.012 h in normal, while in infected chickens the maximum serum concentrations was 132.93 ± 1.53μg/ml attained at maximum time of 1.265 ± 0.013 h. In conclusion a cefepime at dose of 100 mg/kg administered intravenously or IM at 24 h intervals may provide successful treatment of chicken infected with Salmonella typhimurium.Taha A. AttiaSouth Valley UniversityarticleCefepimeSalmonella typhimuriumhigh performance liquid chromatographybroiler chickenAgricultureSVeterinary medicineSF600-1100ENSVU-International Journal of Veterinary Sciences, Vol 1, Iss 1, Pp 66-81 (2018) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Cefepime Salmonella typhimurium high performance liquid chromatography broiler chicken Agriculture S Veterinary medicine SF600-1100 |
spellingShingle |
Cefepime Salmonella typhimurium high performance liquid chromatography broiler chicken Agriculture S Veterinary medicine SF600-1100 Taha A. Attia Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
description |
Cefepime is a broad-spectrum semi synthetic β-lactamase resistant fourth generation cephalosporin. Looking to potential for clinical use, pharmacokinetics of cefepime following single intravenous and intramuscular (IM) dose (100mg/kg b. wt.) in healthy and experimentally Salmonella typhimurium infected broiler chickens were determined. Cefepime concentration in plasma samples was determined by reverse-phase high performance liquid chromatography with mobile phase. The mobile phase was a mixture of 10mM phosphate buffer (pH 7): Methanol; 75:25 was always freshly prepared. Flow rates were 1 ml/min. UV detection was performed at 256 nm, injection volume was 20 µl. After a single intravenous injection, cefepime reached its maximum serum concentrations of 4.28 ± 0.37μg/ml in normal chickens, while in the infected chickens, the maximum serum concentration was 2.62 ± 0.72 μg/ml. Cefepime was eliminated after intravenous injection with half-life (t1/2 β) of 4.608 ± 0.145 h in normal which significantly longer than 4.19 ± 0.158 h in infected chickens. The mean residence time (MRT) was 6.51 ± 0.189h in normal vs 5.86±0.18 h in infected chickens. After IM administration the drug reached its maximum serum concentrations of 193.06 ± 2.27μg/ml at maximum time of 1.138 ± 0.012 h in normal, while in infected chickens the maximum serum concentrations was 132.93 ± 1.53μg/ml attained at maximum time of 1.265 ± 0.013 h. In conclusion a cefepime at dose of 100 mg/kg administered intravenously or IM at 24 h intervals may provide successful treatment of chicken infected with Salmonella typhimurium. |
format |
article |
author |
Taha A. Attia |
author_facet |
Taha A. Attia |
author_sort |
Taha A. Attia |
title |
Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
title_short |
Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
title_full |
Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
title_fullStr |
Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
title_full_unstemmed |
Disposition Kinetics of Cefepime in Healthy and Experimental Salmonella Typhimurium Infected Broiler Chicken |
title_sort |
disposition kinetics of cefepime in healthy and experimental salmonella typhimurium infected broiler chicken |
publisher |
South Valley University |
publishDate |
2018 |
url |
https://doaj.org/article/07f8918873744e4285049b42f1ced7f3 |
work_keys_str_mv |
AT tahaaattia dispositionkineticsofcefepimeinhealthyandexperimentalsalmonellatyphimuriuminfectedbroilerchicken |
_version_ |
1718403476126433280 |