Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation

Acetaldehyde–alcohol dehydrogenase (AdhE) converts acetyl-CoA to ethanol and is a key enzyme in bacterial alcoholic fermentation. AdhE forms spirosomes and, here, the authors present the cryoEM structures of compact and extended E.coli AdhE spirosomes and show that the extended conformation is the c...

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Autores principales:	Pauline Pony, Chiara Rapisarda, Laurent Terradot, Esther Marza, Rémi Fronzes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/07fa1f5397a148c884f80a53704a93fe
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spelling	oai:doaj.org-article:07fa1f5397a148c884f80a53704a93fe2021-12-02T17:31:09ZFilamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation10.1038/s41467-020-15214-y2041-1723https://doaj.org/article/07fa1f5397a148c884f80a53704a93fe2020-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15214-yhttps://doaj.org/toc/2041-1723Acetaldehyde–alcohol dehydrogenase (AdhE) converts acetyl-CoA to ethanol and is a key enzyme in bacterial alcoholic fermentation. AdhE forms spirosomes and, here, the authors present the cryoEM structures of compact and extended E.coli AdhE spirosomes and show that the extended conformation is the catalytically active form of the enzyme and discuss mechanistic implications.Pauline PonyChiara RapisardaLaurent TerradotEsther MarzaRémi FronzesNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Pauline Pony Chiara Rapisarda Laurent Terradot Esther Marza Rémi Fronzes Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation
description	Acetaldehyde–alcohol dehydrogenase (AdhE) converts acetyl-CoA to ethanol and is a key enzyme in bacterial alcoholic fermentation. AdhE forms spirosomes and, here, the authors present the cryoEM structures of compact and extended E.coli AdhE spirosomes and show that the extended conformation is the catalytically active form of the enzyme and discuss mechanistic implications.
format	article
author	Pauline Pony Chiara Rapisarda Laurent Terradot Esther Marza Rémi Fronzes
author_facet	Pauline Pony Chiara Rapisarda Laurent Terradot Esther Marza Rémi Fronzes
author_sort	Pauline Pony
title	Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation
title_short	Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation
title_full	Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation
title_fullStr	Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation
title_full_unstemmed	Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation
title_sort	filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase adhe is essential for substrate channeling and enzymatic regulation
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/07fa1f5397a148c884f80a53704a93fe
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Filamentation of the bacterial bi-functional alcohol/aldehyde dehydrogenase AdhE is essential for substrate channeling and enzymatic regulation

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