The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal

Abstract The morphology of the Golgi complex is influenced by the cellular context, which strictly correlates with nuclear functions; however, the mechanism underlying this association remains elusive. The inner nuclear membrane SUN proteins, SUN1 and SUN2, have diverse functions together with the o...

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Autores principales:	Miki Hieda, Taizo Matsumoto, Mari Isobe, Sadamu Kurono, Kaneko Yuka, Satoshi Kametaka, Jing-Ya Wang, Ya-Hui Chi, Kenji Kameda, Hiroshi Kimura, Nariaki Matsuura, Shuji Matsuura
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/07fb44b567dc435f80af93460cf0e49d
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spelling	oai:doaj.org-article:07fb44b567dc435f80af93460cf0e49d2021-12-02T13:30:12ZThe SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal10.1038/s41598-021-84750-42045-2322https://doaj.org/article/07fb44b567dc435f80af93460cf0e49d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84750-4https://doaj.org/toc/2045-2322Abstract The morphology of the Golgi complex is influenced by the cellular context, which strictly correlates with nuclear functions; however, the mechanism underlying this association remains elusive. The inner nuclear membrane SUN proteins, SUN1 and SUN2, have diverse functions together with the outer nuclear membrane nesprin proteins, which comprise the LINC complex. We found that depletion of SUN1 leads to Golgi complex dispersion with maintenance of ministacks and retained function for vesicle transport through the Golgi complex. In addition, SUN2 associates with microtubule plus-end-directed motor KIF20A, possibly via nesprin-2. KIF20A plays a role in the Golgi dispersion in conjunction with the SUN2-nesprin-2 LINC complex in SUN1-depleted cells, suggesting that SUN1 suppresses the function of the SUN2-nesprin-2 LINC complex under a steady-state condition. Further, SUN1-knockout mice, which show impaired cerebellar development and cerebellar ataxia, presented altered Golgi morphology in Purkinje cells. These findings revealed a regulation of the Golgi organization by the LINC complex.Miki HiedaTaizo MatsumotoMari IsobeSadamu KuronoKaneko YukaSatoshi KametakaJing-Ya WangYa-Hui ChiKenji KamedaHiroshi KimuraNariaki MatsuuraShuji MatsuuraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miki Hieda Taizo Matsumoto Mari Isobe Sadamu Kurono Kaneko Yuka Satoshi Kametaka Jing-Ya Wang Ya-Hui Chi Kenji Kameda Hiroshi Kimura Nariaki Matsuura Shuji Matsuura The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
description	Abstract The morphology of the Golgi complex is influenced by the cellular context, which strictly correlates with nuclear functions; however, the mechanism underlying this association remains elusive. The inner nuclear membrane SUN proteins, SUN1 and SUN2, have diverse functions together with the outer nuclear membrane nesprin proteins, which comprise the LINC complex. We found that depletion of SUN1 leads to Golgi complex dispersion with maintenance of ministacks and retained function for vesicle transport through the Golgi complex. In addition, SUN2 associates with microtubule plus-end-directed motor KIF20A, possibly via nesprin-2. KIF20A plays a role in the Golgi dispersion in conjunction with the SUN2-nesprin-2 LINC complex in SUN1-depleted cells, suggesting that SUN1 suppresses the function of the SUN2-nesprin-2 LINC complex under a steady-state condition. Further, SUN1-knockout mice, which show impaired cerebellar development and cerebellar ataxia, presented altered Golgi morphology in Purkinje cells. These findings revealed a regulation of the Golgi organization by the LINC complex.
format	article
author	Miki Hieda Taizo Matsumoto Mari Isobe Sadamu Kurono Kaneko Yuka Satoshi Kametaka Jing-Ya Wang Ya-Hui Chi Kenji Kameda Hiroshi Kimura Nariaki Matsuura Shuji Matsuura
author_facet	Miki Hieda Taizo Matsumoto Mari Isobe Sadamu Kurono Kaneko Yuka Satoshi Kametaka Jing-Ya Wang Ya-Hui Chi Kenji Kameda Hiroshi Kimura Nariaki Matsuura Shuji Matsuura
author_sort	Miki Hieda
title	The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
title_short	The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
title_full	The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
title_fullStr	The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
title_full_unstemmed	The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
title_sort	sun2-nesprin-2 linc complex and kif20a function in the golgi dispersal
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/07fb44b567dc435f80af93460cf0e49d
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The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal

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