Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity

Belete Kassa Alemu, Desye Misganaw, Getnet Mengistu Pharmacology and Toxicology Unit, Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaCorrespondence: Belete Kassa AlemuPharmacology and Toxicology Unit, Department of Pharmacy, College of Medicine and...

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Autores principales:	Alemu BK, Misganaw D, Mengistu G
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	crude extract epithelialization breaking strength wound contraction a.schimperi Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/08049f65dc554fcb893f182ab7497f14
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spelling	oai:doaj.org-article:08049f65dc554fcb893f182ab7497f142021-12-02T15:06:10ZWound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity1179-1438https://doaj.org/article/08049f65dc554fcb893f182ab7497f142020-12-01T00:00:00Zhttps://www.dovepress.com/wound-healing-effect-of-acokanthera-schimperi-schweinf-apocynaceae-met-peer-reviewed-article-CPAAhttps://doaj.org/toc/1179-1438Belete Kassa Alemu, Desye Misganaw, Getnet Mengistu Pharmacology and Toxicology Unit, Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaCorrespondence: Belete Kassa AlemuPharmacology and Toxicology Unit, Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaTel +251 918330307Fax +251 333115052Email belete.kassa@wu.edu.etBackground: Acokanthera schimperi is traditionally used for the treatment of wounds and various bacterial infections. Due to the ongoing escalation of antimicrobial resistance, there is an increasing demand for the appropriate wound care and hence, the present study was initiated to investigate the wound healing effects of the leaf extract ointments of A. schimperi in mice and its in-vitro antioxidant activity.Methods: The crude extract was prepared as 5% and 10% w/w ointments for topical use in mice. Wound contraction and epithelialization period were determined in excision and infected models, whereas tensile strength was determined in an incision model. Besides, its antioxidant activity was evaluated using the DPPH method.Results: In this study, the 10% w/w extract ointment did not cause toxicity at the 2000 mg/kg limit dose. In the excision model, the 10% w/w ointment exhibited a significant wound contraction effect starting from day 6 to 14 with a complete epithelization shown on day 13. Besides, the 5%w/w ointment showed a significant wound contraction effect starting from day 6 onwards, and a significant decrease in the epithelization period observed on day 16. Conversely, both the 10% w/w and 5% w/w ointments showed significant wound contraction effects starting from day 4 and onwards in the infected model. However, a complete epithelization period was observed on days 14 and 18 in the 10%w/w and 5% w/w/extract ointment treated groups, respectively. In the incision model, the 10% (w/w) and 5% (w/w) extract ointments showed a significant increase in tensile strength by 36.80 and 32.23%, respectively. Moreover, the antioxidant activity of the extract was concentration-dependent with an IC50 value of 5.49± 0.38 μg/μL.Conclusion: The potential wound healing effects of this plant may provide a candidate source in the discovery of new drugs for the treatment of wounds.Keywords: wound healing, antioxidant activity, extract ointments A. schimperiAlemu BKMisganaw DMengistu GDove Medical Pressarticlecrude extractepithelializationbreaking strengthwound contractiona.schimperiTherapeutics. PharmacologyRM1-950ENClinical Pharmacology: Advances and Applications, Vol Volume 12, Pp 213-222 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	crude extract epithelialization breaking strength wound contraction a.schimperi Therapeutics. Pharmacology RM1-950
spellingShingle	crude extract epithelialization breaking strength wound contraction a.schimperi Therapeutics. Pharmacology RM1-950 Alemu BK Misganaw D Mengistu G Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity
description	Belete Kassa Alemu, Desye Misganaw, Getnet Mengistu Pharmacology and Toxicology Unit, Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaCorrespondence: Belete Kassa AlemuPharmacology and Toxicology Unit, Department of Pharmacy, College of Medicine and Health Sciences, Wollo University, Dessie, EthiopiaTel +251 918330307Fax +251 333115052Email belete.kassa@wu.edu.etBackground: Acokanthera schimperi is traditionally used for the treatment of wounds and various bacterial infections. Due to the ongoing escalation of antimicrobial resistance, there is an increasing demand for the appropriate wound care and hence, the present study was initiated to investigate the wound healing effects of the leaf extract ointments of A. schimperi in mice and its in-vitro antioxidant activity.Methods: The crude extract was prepared as 5% and 10% w/w ointments for topical use in mice. Wound contraction and epithelialization period were determined in excision and infected models, whereas tensile strength was determined in an incision model. Besides, its antioxidant activity was evaluated using the DPPH method.Results: In this study, the 10% w/w extract ointment did not cause toxicity at the 2000 mg/kg limit dose. In the excision model, the 10% w/w ointment exhibited a significant wound contraction effect starting from day 6 to 14 with a complete epithelization shown on day 13. Besides, the 5%w/w ointment showed a significant wound contraction effect starting from day 6 onwards, and a significant decrease in the epithelization period observed on day 16. Conversely, both the 10% w/w and 5% w/w ointments showed significant wound contraction effects starting from day 4 and onwards in the infected model. However, a complete epithelization period was observed on days 14 and 18 in the 10%w/w and 5% w/w/extract ointment treated groups, respectively. In the incision model, the 10% (w/w) and 5% (w/w) extract ointments showed a significant increase in tensile strength by 36.80 and 32.23%, respectively. Moreover, the antioxidant activity of the extract was concentration-dependent with an IC50 value of 5.49± 0.38 μg/μL.Conclusion: The potential wound healing effects of this plant may provide a candidate source in the discovery of new drugs for the treatment of wounds.Keywords: wound healing, antioxidant activity, extract ointments A. schimperi
format	article
author	Alemu BK Misganaw D Mengistu G
author_facet	Alemu BK Misganaw D Mengistu G
author_sort	Alemu BK
title	Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity
title_short	Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity
title_full	Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity
title_fullStr	Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity
title_full_unstemmed	Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity
title_sort	wound healing effect of acokanthera schimperi schweinf (apocynaceae) methanol leaf extract ointment in mice and its in-vitro antioxidant activity
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/08049f65dc554fcb893f182ab7497f14
work_keys_str_mv	AT alemubk woundhealingeffectofacokantheraschimperischweinfapocynaceaemethanolleafextractointmentinmiceanditsinvitroantioxidantactivity AT misganawd woundhealingeffectofacokantheraschimperischweinfapocynaceaemethanolleafextractointmentinmiceanditsinvitroantioxidantactivity AT mengistug woundhealingeffectofacokantheraschimperischweinfapocynaceaemethanolleafextractointmentinmiceanditsinvitroantioxidantactivity
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Wound Healing Effect of Acokanthera schimperi Schweinf (Apocynaceae) Methanol Leaf Extract Ointment in Mice and Its in-vitro Antioxidant Activity

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