Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy

Polarization microscopy has been combined with single-molecule localization, but it’s often limited in either speed or resolution. Here the authors present polarized Structured Illumination Microscopy (pSIM), a method that uses polarized laser excitation to measure dye orientation during fast super-...

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Autores principales: Karl Zhanghao, Xingye Chen, Wenhui Liu, Meiqi Li, Yiqiong Liu, Yiming Wang, Sha Luo, Xiao Wang, Chunyan Shan, Hao Xie, Juntao Gao, Xiaowei Chen, Dayong Jin, Xiangdong Li, Yan Zhang, Qionghai Dai, Peng Xi
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Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/080c2362f4c04472a1ce4e4338f97ada
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spelling oai:doaj.org-article:080c2362f4c04472a1ce4e4338f97ada2021-12-02T16:58:27ZSuper-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy10.1038/s41467-019-12681-w2041-1723https://doaj.org/article/080c2362f4c04472a1ce4e4338f97ada2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12681-whttps://doaj.org/toc/2041-1723Polarization microscopy has been combined with single-molecule localization, but it’s often limited in either speed or resolution. Here the authors present polarized Structured Illumination Microscopy (pSIM), a method that uses polarized laser excitation to measure dye orientation during fast super-resolution live cell imaging.Karl ZhanghaoXingye ChenWenhui LiuMeiqi LiYiqiong LiuYiming WangSha LuoXiao WangChunyan ShanHao XieJuntao GaoXiaowei ChenDayong JinXiangdong LiYan ZhangQionghai DaiPeng XiNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Karl Zhanghao
Xingye Chen
Wenhui Liu
Meiqi Li
Yiqiong Liu
Yiming Wang
Sha Luo
Xiao Wang
Chunyan Shan
Hao Xie
Juntao Gao
Xiaowei Chen
Dayong Jin
Xiangdong Li
Yan Zhang
Qionghai Dai
Peng Xi
Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
description Polarization microscopy has been combined with single-molecule localization, but it’s often limited in either speed or resolution. Here the authors present polarized Structured Illumination Microscopy (pSIM), a method that uses polarized laser excitation to measure dye orientation during fast super-resolution live cell imaging.
format article
author Karl Zhanghao
Xingye Chen
Wenhui Liu
Meiqi Li
Yiqiong Liu
Yiming Wang
Sha Luo
Xiao Wang
Chunyan Shan
Hao Xie
Juntao Gao
Xiaowei Chen
Dayong Jin
Xiangdong Li
Yan Zhang
Qionghai Dai
Peng Xi
author_facet Karl Zhanghao
Xingye Chen
Wenhui Liu
Meiqi Li
Yiqiong Liu
Yiming Wang
Sha Luo
Xiao Wang
Chunyan Shan
Hao Xie
Juntao Gao
Xiaowei Chen
Dayong Jin
Xiangdong Li
Yan Zhang
Qionghai Dai
Peng Xi
author_sort Karl Zhanghao
title Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
title_short Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
title_full Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
title_fullStr Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
title_full_unstemmed Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
title_sort super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/080c2362f4c04472a1ce4e4338f97ada
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