Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy
Polarization microscopy has been combined with single-molecule localization, but it’s often limited in either speed or resolution. Here the authors present polarized Structured Illumination Microscopy (pSIM), a method that uses polarized laser excitation to measure dye orientation during fast super-...
Guardado en:
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | article |
Lenguaje: | EN |
Publicado: |
Nature Portfolio
2019
|
Materias: | |
Acceso en línea: | https://doaj.org/article/080c2362f4c04472a1ce4e4338f97ada |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
id |
oai:doaj.org-article:080c2362f4c04472a1ce4e4338f97ada |
---|---|
record_format |
dspace |
spelling |
oai:doaj.org-article:080c2362f4c04472a1ce4e4338f97ada2021-12-02T16:58:27ZSuper-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy10.1038/s41467-019-12681-w2041-1723https://doaj.org/article/080c2362f4c04472a1ce4e4338f97ada2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12681-whttps://doaj.org/toc/2041-1723Polarization microscopy has been combined with single-molecule localization, but it’s often limited in either speed or resolution. Here the authors present polarized Structured Illumination Microscopy (pSIM), a method that uses polarized laser excitation to measure dye orientation during fast super-resolution live cell imaging.Karl ZhanghaoXingye ChenWenhui LiuMeiqi LiYiqiong LiuYiming WangSha LuoXiao WangChunyan ShanHao XieJuntao GaoXiaowei ChenDayong JinXiangdong LiYan ZhangQionghai DaiPeng XiNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-10 (2019) |
institution |
DOAJ |
collection |
DOAJ |
language |
EN |
topic |
Science Q |
spellingShingle |
Science Q Karl Zhanghao Xingye Chen Wenhui Liu Meiqi Li Yiqiong Liu Yiming Wang Sha Luo Xiao Wang Chunyan Shan Hao Xie Juntao Gao Xiaowei Chen Dayong Jin Xiangdong Li Yan Zhang Qionghai Dai Peng Xi Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy |
description |
Polarization microscopy has been combined with single-molecule localization, but it’s often limited in either speed or resolution. Here the authors present polarized Structured Illumination Microscopy (pSIM), a method that uses polarized laser excitation to measure dye orientation during fast super-resolution live cell imaging. |
format |
article |
author |
Karl Zhanghao Xingye Chen Wenhui Liu Meiqi Li Yiqiong Liu Yiming Wang Sha Luo Xiao Wang Chunyan Shan Hao Xie Juntao Gao Xiaowei Chen Dayong Jin Xiangdong Li Yan Zhang Qionghai Dai Peng Xi |
author_facet |
Karl Zhanghao Xingye Chen Wenhui Liu Meiqi Li Yiqiong Liu Yiming Wang Sha Luo Xiao Wang Chunyan Shan Hao Xie Juntao Gao Xiaowei Chen Dayong Jin Xiangdong Li Yan Zhang Qionghai Dai Peng Xi |
author_sort |
Karl Zhanghao |
title |
Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy |
title_short |
Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy |
title_full |
Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy |
title_fullStr |
Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy |
title_full_unstemmed |
Super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy |
title_sort |
super-resolution imaging of fluorescent dipoles via polarized structured illumination microscopy |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/080c2362f4c04472a1ce4e4338f97ada |
work_keys_str_mv |
AT karlzhanghao superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT xingyechen superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT wenhuiliu superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT meiqili superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT yiqiongliu superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT yimingwang superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT shaluo superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT xiaowang superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT chunyanshan superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT haoxie superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT juntaogao superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT xiaoweichen superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT dayongjin superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT xiangdongli superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT yanzhang superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT qionghaidai superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy AT pengxi superresolutionimagingoffluorescentdipolesviapolarizedstructuredilluminationmicroscopy |
_version_ |
1718382309072175104 |