Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre

Introduction: Identification of Extended spectrum beta lactamases (ESBL), AmpC production and carbapenemase production among isolates of Escherichia coli, helps clinician to rationalize the choice of antibiotics. However, there is a lack of simple and effective method for simultaneous identification...

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Autores principales:	Uma Chaudhary, Shipra Agarwal, Kausalya Raghuraman
Formato:	article
Lenguaje:	EN
Publicado:	Thieme Medical and Scientific Publishers Pvt. Ltd. 2018
Materias:	ampc carbapenemases extended spectrum beta lactamases twelve disc method Medicine R
Acceso en línea:	https://doaj.org/article/080f5a61fb024ba79c631dda16048cbd
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spelling	oai:doaj.org-article:080f5a61fb024ba79c631dda16048cbd2021-12-02T17:07:47ZIdentification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre2231-07702249-446410.4103/ajm.AJM_156_17https://doaj.org/article/080f5a61fb024ba79c631dda16048cbd2018-04-01T00:00:00Zhttp://www.thieme-connect.de/DOI/DOI?10.4103/ajm.AJM_156_17https://doaj.org/toc/2231-0770https://doaj.org/toc/2249-4464Introduction: Identification of Extended spectrum beta lactamases (ESBL), AmpC production and carbapenemase production among isolates of Escherichia coli, helps clinician to rationalize the choice of antibiotics. However, there is a lack of simple and effective method for simultaneous identification of these beta lactamases.Aim: To determine the concurrent production of beta lactamases using twelve disc method on E. coli isolates.Materials and Methods: A total of 200 multidrug resistant E. coli were screened using twelve disc method. The isolates of ESBL were confirmed by ceftazidime/clavulanic acid and cefotaxime/clavulanic acid method. Metallo-beta-lactamases (MBL) were confirmed by imipenem EDTA combined disc method.Results: Among the 200 isolates, 42.5% were ESBL producers, 9% were MBL and 6.5% were Klebsiella pneumoniae carbapenemase (KPC) and AmpC each respectively. Coproduction was seen in 54 (27%). A significant difference in sensitivity was seen in cefuroxime, aztreonam, cefoxitin and ceftriaxone among inpatient and outpatients.Conclusion: The present study highlights burden of ESBL, AmpC, KPC and MBL along with their coproduction in a tertiary care hospital. In-house antibiotic policy, infection control and epidemiological surveys will help us in controlling these resistant bugs. We believe, the twelve disc method is a simple, inexpensive screening method for beta lactamase production.Uma ChaudharyShipra AgarwalKausalya RaghuramanThieme Medical and Scientific Publishers Pvt. Ltd.articleampccarbapenemasesextended spectrum beta lactamasestwelve disc methodMedicineRENAvicenna Journal of Medicine, Vol 8, Iss 02, Pp 46-50 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	ampc carbapenemases extended spectrum beta lactamases twelve disc method Medicine R
spellingShingle	ampc carbapenemases extended spectrum beta lactamases twelve disc method Medicine R Uma Chaudhary Shipra Agarwal Kausalya Raghuraman Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre
description	Introduction: Identification of Extended spectrum beta lactamases (ESBL), AmpC production and carbapenemase production among isolates of Escherichia coli, helps clinician to rationalize the choice of antibiotics. However, there is a lack of simple and effective method for simultaneous identification of these beta lactamases.Aim: To determine the concurrent production of beta lactamases using twelve disc method on E. coli isolates.Materials and Methods: A total of 200 multidrug resistant E. coli were screened using twelve disc method. The isolates of ESBL were confirmed by ceftazidime/clavulanic acid and cefotaxime/clavulanic acid method. Metallo-beta-lactamases (MBL) were confirmed by imipenem EDTA combined disc method.Results: Among the 200 isolates, 42.5% were ESBL producers, 9% were MBL and 6.5% were Klebsiella pneumoniae carbapenemase (KPC) and AmpC each respectively. Coproduction was seen in 54 (27%). A significant difference in sensitivity was seen in cefuroxime, aztreonam, cefoxitin and ceftriaxone among inpatient and outpatients.Conclusion: The present study highlights burden of ESBL, AmpC, KPC and MBL along with their coproduction in a tertiary care hospital. In-house antibiotic policy, infection control and epidemiological surveys will help us in controlling these resistant bugs. We believe, the twelve disc method is a simple, inexpensive screening method for beta lactamase production.
format	article
author	Uma Chaudhary Shipra Agarwal Kausalya Raghuraman
author_facet	Uma Chaudhary Shipra Agarwal Kausalya Raghuraman
author_sort	Uma Chaudhary
title	Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre
title_short	Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre
title_full	Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre
title_fullStr	Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre
title_full_unstemmed	Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre
title_sort	identification of extended spectrum beta lactamases, ampc and carbapenemase production among isolates of escherichia coli in north indian tertiary care centre
publisher	Thieme Medical and Scientific Publishers Pvt. Ltd.
publishDate	2018
url	https://doaj.org/article/080f5a61fb024ba79c631dda16048cbd
work_keys_str_mv	AT umachaudhary identificationofextendedspectrumbetalactamasesampcandcarbapenemaseproductionamongisolatesofescherichiacoliinnorthindiantertiarycarecentre AT shipraagarwal identificationofextendedspectrumbetalactamasesampcandcarbapenemaseproductionamongisolatesofescherichiacoliinnorthindiantertiarycarecentre AT kausalyaraghuraman identificationofextendedspectrumbetalactamasesampcandcarbapenemaseproductionamongisolatesofescherichiacoliinnorthindiantertiarycarecentre
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Identification of extended spectrum beta lactamases, AmpC and carbapenemase production among isolates of Escherichia coli in North Indian tertiary care centre

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