The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana
Odorant-binding proteins participate in the olfactory system of the honeybee. <em>Apis mellifera</em> ligustica and <em>Apis cerana </em>cerana are species of honeybee that have different biologic functions. The two species have diversified olfactory systems, with <em>A...
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| Autores principales: | , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Universidade Estadual de Feira de Santana
2014
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/0810bd25e25649b4be4dbc1ca797ec07 |
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| Sumario: | Odorant-binding proteins participate in the olfactory system of the honeybee. <em>Apis mellifera</em> ligustica and <em>Apis cerana </em>cerana are species of honeybee that have different biologic functions. The two species have diversified olfactory systems, with <em>A. cerana</em> displaying sensitive olfactory involvement in collecting nectar and pollen from small plants; and<em> A. mellifera</em> collecting from large nectariferous plants. We hypothesized that, given this difference in biologic activity, the gene <em>obp11</em> of <em>A. mellifera</em> and <em>A. cerana</em> may show different olfactory expression patterns. We cloned and sequenced the <em>obp11</em> genes from <em>A. mellifera (Amobp11)</em> and <em>A. cerana (Acobp11)</em>. Using quantitative real-time PCR, we demonstrated that nurse workers, which have the highest olfactory sensitivity in the <em>A. mellifera</em> hive, have the highest expression of<em> Amobp11</em>; whereas 1-day-emerged workers, which have lowest olfactory sensitivity, have correspondingly low expression. However, the highest expression of <em>Acobp11</em> is observed for foragers, which display the highest olfactory sensitivity in the <em>A. cerana</em> population. The OBP11 protein from the two species is highly conserved, with an apparent molecular weight and predicted extracellular localization that is similar to other OBP proteins. The expression of the <em>obp11</em> gene in <em>A. mellifera</em> and <em>A. cerana</em> correlates with the different roles of the olfactory system for the two different species. These findings support the critical role of odorant-binding proteins in the <em>Apis</em> olfactory system |
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