The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana

Odorant-binding proteins participate in the olfactory system of the honeybee. Apis mellifera ligustica and Apis cerana cerana are species of honeybee that have different biologic functions. The two species have diversified olfactory systems, with A...

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Autores principales:	Hongxia Zhao, Yuexiong Luo, Jianghong Lee, Xuefeng Zhang, qin Liang, Xinnian Zeng
Formato:	article
Lenguaje:	EN
Publicado:	Universidade Estadual de Feira de Santana 2014
Materias:	odorant-binding protein olfactory Apis Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5
Acceso en línea:	https://doaj.org/article/0810bd25e25649b4be4dbc1ca797ec07
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spelling	oai:doaj.org-article:0810bd25e25649b4be4dbc1ca797ec072021-12-02T10:52:10ZThe Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana0361-652510.13102/sociobiology.v60i4.429-435https://doaj.org/article/0810bd25e25649b4be4dbc1ca797ec072014-01-01T00:00:00Zhttp://periodicos.uefs.br/ojs/index.php/sociobiology/article/view/39https://doaj.org/toc/0361-6525Odorant-binding proteins participate in the olfactory system of the honeybee. <em>Apis mellifera</em> ligustica and <em>Apis cerana </em>cerana are species of honeybee that have different biologic functions. The two species have diversified olfactory systems, with <em>A. cerana</em> displaying sensitive olfactory involvement in collecting nectar and pollen from small plants; and<em> A. mellifera</em> collecting from large nectariferous plants. We hypothesized that, given this difference in biologic activity, the gene <em>obp11</em> of <em>A. mellifera</em> and <em>A. cerana</em> may show different olfactory expression patterns. We cloned and sequenced the <em>obp11</em> genes from <em>A. mellifera (Amobp11)</em> and <em>A. cerana (Acobp11)</em>. Using quantitative real-time PCR, we demonstrated that nurse workers, which have the highest olfactory sensitivity in the <em>A. mellifera</em> hive, have the highest expression of<em> Amobp11</em>; whereas 1-day-emerged workers, which have lowest olfactory sensitivity, have correspondingly low expression. However, the highest expression of <em>Acobp11</em> is observed for foragers, which display the highest olfactory sensitivity in the <em>A. cerana</em> population. The OBP11 protein from the two species is highly conserved, with an apparent molecular weight and predicted extracellular localization that is similar to other OBP proteins. The expression of the <em>obp11</em> gene in <em>A. mellifera</em> and <em>A. cerana</em> correlates with the different roles of the olfactory system for the two different species. These findings support the critical role of odorant-binding proteins in the <em>Apis</em> olfactory systemHongxia ZhaoYuexiong LuoJianghong LeeXuefeng Zhangqin LiangXinnian ZengUniversidade Estadual de Feira de Santanaarticleodorant-binding proteinolfactoryApisZoologyQL1-991EcologyQH540-549.5Natural history (General)QH1-278.5ENSociobiology, Vol 60, Iss 4, Pp 429-435 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	odorant-binding protein olfactory Apis Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5
spellingShingle	odorant-binding protein olfactory Apis Zoology QL1-991 Ecology QH540-549.5 Natural history (General) QH1-278.5 Hongxia Zhao Yuexiong Luo Jianghong Lee Xuefeng Zhang qin Liang Xinnian Zeng The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana
description	Odorant-binding proteins participate in the olfactory system of the honeybee. <em>Apis mellifera</em> ligustica and <em>Apis cerana </em>cerana are species of honeybee that have different biologic functions. The two species have diversified olfactory systems, with <em>A. cerana</em> displaying sensitive olfactory involvement in collecting nectar and pollen from small plants; and<em> A. mellifera</em> collecting from large nectariferous plants. We hypothesized that, given this difference in biologic activity, the gene <em>obp11</em> of <em>A. mellifera</em> and <em>A. cerana</em> may show different olfactory expression patterns. We cloned and sequenced the <em>obp11</em> genes from <em>A. mellifera (Amobp11)</em> and <em>A. cerana (Acobp11)</em>. Using quantitative real-time PCR, we demonstrated that nurse workers, which have the highest olfactory sensitivity in the <em>A. mellifera</em> hive, have the highest expression of<em> Amobp11</em>; whereas 1-day-emerged workers, which have lowest olfactory sensitivity, have correspondingly low expression. However, the highest expression of <em>Acobp11</em> is observed for foragers, which display the highest olfactory sensitivity in the <em>A. cerana</em> population. The OBP11 protein from the two species is highly conserved, with an apparent molecular weight and predicted extracellular localization that is similar to other OBP proteins. The expression of the <em>obp11</em> gene in <em>A. mellifera</em> and <em>A. cerana</em> correlates with the different roles of the olfactory system for the two different species. These findings support the critical role of odorant-binding proteins in the <em>Apis</em> olfactory system
format	article
author	Hongxia Zhao Yuexiong Luo Jianghong Lee Xuefeng Zhang qin Liang Xinnian Zeng
author_facet	Hongxia Zhao Yuexiong Luo Jianghong Lee Xuefeng Zhang qin Liang Xinnian Zeng
author_sort	Hongxia Zhao
title	The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana
title_short	The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana
title_full	The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana
title_fullStr	The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana
title_full_unstemmed	The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana
title_sort	odorant-binding protein gene obp11 shows different spatiotemporal roles in the olfactory system of apis mellifera ligustica and apis cerana cerana
publisher	Universidade Estadual de Feira de Santana
publishDate	2014
url	https://doaj.org/article/0810bd25e25649b4be4dbc1ca797ec07
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The Odorant-Binding Protein Gene obp11 Shows Different Spatiotemporal Roles in the Olfactory System of Apis mellifera ligustica and Apis cerana cerana

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