Identification and antiviral effect of Cherry Valley duck IRF4

ABSTRACT: Interferon regulatory factor 4 (IRF4) is a multifunctional transcription factor that plays an important regulatory role in the interferon (IFN) signaling. IRF4 participates in the process of antivirus, Th cell differentiation and B cell maturation by regulating the expression of IFN and so...

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Autores principales:	Xinyu Zhai, Tianqi Hong, Tingting Zhang, Bin Xing, Jinchao Wang, Xiuyuan Wang, Runchun Miao, Tianxu Li, Liangmeng Wei
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	IRF4 cloning Cherry Valley duck innate immunity Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/0818a34e59a640949c6cbeb2ca19a855
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spelling	oai:doaj.org-article:0818a34e59a640949c6cbeb2ca19a8552021-11-24T04:23:19ZIdentification and antiviral effect of Cherry Valley duck IRF40032-579110.1016/j.psj.2021.101560https://doaj.org/article/0818a34e59a640949c6cbeb2ca19a8552022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0032579121005824https://doaj.org/toc/0032-5791ABSTRACT: Interferon regulatory factor 4 (IRF4) is a multifunctional transcription factor that plays an important regulatory role in the interferon (IFN) signaling. IRF4 participates in the process of antivirus, Th cell differentiation and B cell maturation by regulating the expression of IFN and some lymphokines. In this study, Cherry Valley duck IRF4 (duIRF4) was cloned and its cDNA was analyzed. Expression of duIRF4 in a wide variety of tissues and changes in duIRF4 expression due to viral infection also was detected by quantitative real-time PCR. The results show that duIRF4 contains 1,341 bp of ORF encoding a protein with 446 amino acids and contains 3 domains: DNA-binding domain (DBD), IRF-association domain (IAD) and nuclear localization signal (NLS). Quantitative real-time PCR analysis showed that duIRF4 was evenly expressed in all tissues examined, with the highest expression in the spleen, followed by the bursa of Fabricius, and lower in the skin and brain. In addition, expression of duIRF4 in the brain and spleen was significantly upregulated after being infected by duck plague virus, duck Tembusu virus, and novel duck reovirus. These data suggest that duIRF4 may be involved in innate immune response.Xinyu ZhaiTianqi HongTingting ZhangBin XingJinchao WangXiuyuan WangRunchun MiaoTianxu LiLiangmeng WeiElsevierarticleIRF4cloningCherry Valley duckinnate immunityAnimal cultureSF1-1100ENPoultry Science, Vol 101, Iss 1, Pp 101560- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	IRF4 cloning Cherry Valley duck innate immunity Animal culture SF1-1100
spellingShingle	IRF4 cloning Cherry Valley duck innate immunity Animal culture SF1-1100 Xinyu Zhai Tianqi Hong Tingting Zhang Bin Xing Jinchao Wang Xiuyuan Wang Runchun Miao Tianxu Li Liangmeng Wei Identification and antiviral effect of Cherry Valley duck IRF4
description	ABSTRACT: Interferon regulatory factor 4 (IRF4) is a multifunctional transcription factor that plays an important regulatory role in the interferon (IFN) signaling. IRF4 participates in the process of antivirus, Th cell differentiation and B cell maturation by regulating the expression of IFN and some lymphokines. In this study, Cherry Valley duck IRF4 (duIRF4) was cloned and its cDNA was analyzed. Expression of duIRF4 in a wide variety of tissues and changes in duIRF4 expression due to viral infection also was detected by quantitative real-time PCR. The results show that duIRF4 contains 1,341 bp of ORF encoding a protein with 446 amino acids and contains 3 domains: DNA-binding domain (DBD), IRF-association domain (IAD) and nuclear localization signal (NLS). Quantitative real-time PCR analysis showed that duIRF4 was evenly expressed in all tissues examined, with the highest expression in the spleen, followed by the bursa of Fabricius, and lower in the skin and brain. In addition, expression of duIRF4 in the brain and spleen was significantly upregulated after being infected by duck plague virus, duck Tembusu virus, and novel duck reovirus. These data suggest that duIRF4 may be involved in innate immune response.
format	article
author	Xinyu Zhai Tianqi Hong Tingting Zhang Bin Xing Jinchao Wang Xiuyuan Wang Runchun Miao Tianxu Li Liangmeng Wei
author_facet	Xinyu Zhai Tianqi Hong Tingting Zhang Bin Xing Jinchao Wang Xiuyuan Wang Runchun Miao Tianxu Li Liangmeng Wei
author_sort	Xinyu Zhai
title	Identification and antiviral effect of Cherry Valley duck IRF4
title_short	Identification and antiviral effect of Cherry Valley duck IRF4
title_full	Identification and antiviral effect of Cherry Valley duck IRF4
title_fullStr	Identification and antiviral effect of Cherry Valley duck IRF4
title_full_unstemmed	Identification and antiviral effect of Cherry Valley duck IRF4
title_sort	identification and antiviral effect of cherry valley duck irf4
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/0818a34e59a640949c6cbeb2ca19a855
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Identification and antiviral effect of Cherry Valley duck IRF4

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