Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China

Soil microorganisms play an important role in regulating a variety of ecological functions. In recent years, the research on ecological restoration after mining has made people more aware of the importance of microbial diversity to ecosystem restoration. The present study investigated the effect of...

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Autores principales:	Li Fan, Weiping Zhao, Wendan Feng, Ping Mo, Yunlin Zhao, Guiyan Yang, Zhenggang Xu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	microbial diversity bacterial community structure illumina MiSeq sequencing mining area soil Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/0818a3a154db4892984cde48265a6e0c
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spelling	oai:doaj.org-article:0818a3a154db4892984cde48265a6e0c2021-11-11T19:25:09ZInsight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China10.3390/su1321116842071-1050https://doaj.org/article/0818a3a154db4892984cde48265a6e0c2021-10-01T00:00:00Zhttps://www.mdpi.com/2071-1050/13/21/11684https://doaj.org/toc/2071-1050Soil microorganisms play an important role in regulating a variety of ecological functions. In recent years, the research on ecological restoration after mining has made people more aware of the importance of microbial diversity to ecosystem restoration. The present study investigated the effect of ecological restoration on microbial community structure and its relationship with soil physicochemical properties in the Dabaoshan mining area, China. High throughput sequencing technology was used to analyze and compare the microbial community composition of three types of soil (undamaged area, unrestoration area, and ecological restoration area). The contents of organic carbon, total nitrogen, and total phosphorus were 2.38–12.97 g/kg, 0.39–1.62 g/kg, and 0.99–1.51 g/kg, respectively. In different soil states, undamaged area and ecological restoration area were significantly higher than those in unrestoration area. The results showed that the structure of soil microbial community was significantly correlated with soil physicochemical properties, and formations in the repaired and unrepaired soils were different. Operational Taxonomic Unit (OTU) cluster analysis and diversity index analysis showed that soil microbial community changed at phylum and genus levels. The results showed that at the phylum level, all soil samples contained Firmicutes, Proteobacteria, and actinobacteria. Firmicutes and Proteobacteria of the ecological restoration area (ER1, ER2) were the highest in relative abundance compared with other samples, accounting for more than 45%. Proteobacteria and Acidobacteria were the dominant phylum in the undamaged area (UD), accounting for 32.7% and 22.3%, respectively. It can be seen that soil restoration produced a new dominant population, and Proteobacteria showed an absolute competitive advantage in the mining soil.Li FanWeiping ZhaoWendan FengPing MoYunlin ZhaoGuiyan YangZhenggang XuMDPI AGarticlemicrobial diversitybacterial community structureillumina MiSeq sequencingmining area soilEnvironmental effects of industries and plantsTD194-195Renewable energy sourcesTJ807-830Environmental sciencesGE1-350ENSustainability, Vol 13, Iss 11684, p 11684 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbial diversity bacterial community structure illumina MiSeq sequencing mining area soil Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350
spellingShingle	microbial diversity bacterial community structure illumina MiSeq sequencing mining area soil Environmental effects of industries and plants TD194-195 Renewable energy sources TJ807-830 Environmental sciences GE1-350 Li Fan Weiping Zhao Wendan Feng Ping Mo Yunlin Zhao Guiyan Yang Zhenggang Xu Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China
description	Soil microorganisms play an important role in regulating a variety of ecological functions. In recent years, the research on ecological restoration after mining has made people more aware of the importance of microbial diversity to ecosystem restoration. The present study investigated the effect of ecological restoration on microbial community structure and its relationship with soil physicochemical properties in the Dabaoshan mining area, China. High throughput sequencing technology was used to analyze and compare the microbial community composition of three types of soil (undamaged area, unrestoration area, and ecological restoration area). The contents of organic carbon, total nitrogen, and total phosphorus were 2.38–12.97 g/kg, 0.39–1.62 g/kg, and 0.99–1.51 g/kg, respectively. In different soil states, undamaged area and ecological restoration area were significantly higher than those in unrestoration area. The results showed that the structure of soil microbial community was significantly correlated with soil physicochemical properties, and formations in the repaired and unrepaired soils were different. Operational Taxonomic Unit (OTU) cluster analysis and diversity index analysis showed that soil microbial community changed at phylum and genus levels. The results showed that at the phylum level, all soil samples contained Firmicutes, Proteobacteria, and actinobacteria. Firmicutes and Proteobacteria of the ecological restoration area (ER1, ER2) were the highest in relative abundance compared with other samples, accounting for more than 45%. Proteobacteria and Acidobacteria were the dominant phylum in the undamaged area (UD), accounting for 32.7% and 22.3%, respectively. It can be seen that soil restoration produced a new dominant population, and Proteobacteria showed an absolute competitive advantage in the mining soil.
format	article
author	Li Fan Weiping Zhao Wendan Feng Ping Mo Yunlin Zhao Guiyan Yang Zhenggang Xu
author_facet	Li Fan Weiping Zhao Wendan Feng Ping Mo Yunlin Zhao Guiyan Yang Zhenggang Xu
author_sort	Li Fan
title	Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China
title_short	Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China
title_full	Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China
title_fullStr	Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China
title_full_unstemmed	Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China
title_sort	insight into the characteristics of soil microbial diversity during the ecological restoration of mines: a case study in dabaoshan mining area, china
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/0818a3a154db4892984cde48265a6e0c
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Insight into the Characteristics of Soil Microbial Diversity during the Ecological Restoration of Mines: A Case Study in Dabaoshan Mining Area, China

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