Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration

Introduction: Biomechanical stimulation of cultured human osteoblast-like cells, which is based on controlled mechanical vibration, has been previously indicated, but the exact mechanical parameters that are effective for cells' proliferation enhancement are still elusive due to the lack of dir...

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Autores principales:	Nahum Rosenberg, Orit Rosenberg, Jacob Halevi Politch, Haim Abramovich
Formato:	article
Lenguaje:	EN
Publicado:	Emergency Department of Hospital San Pedro (Logroño, Spain) 2021
Materias:	osteoblast mechanical stimulation vibration mechanotransduction Medicine (General) R5-920
Acceso en línea:	https://doi.org/10.5281/zenodo.4746404 https://doaj.org/article/081aec6b67d6443396c83b6a3bf07397
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spelling	oai:doaj.org-article:081aec6b67d6443396c83b6a3bf073972021-12-02T19:19:53ZOptimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibrationhttps://doi.org/10.5281/zenodo.47464042695-5075https://doaj.org/article/081aec6b67d6443396c83b6a3bf073972021-05-01T00:00:00Zhttps://doi.org/10.5281/zenodo.4746404https://doaj.org/toc/2695-5075Introduction: Biomechanical stimulation of cultured human osteoblast-like cells, which is based on controlled mechanical vibration, has been previously indicated, but the exact mechanical parameters that are effective for cells' proliferation enhancement are still elusive due to the lack of direct data recordings from the stimulated cells in culture. Therefore, we developed a low friction tunable system that enables recording of a narrow range of mechanical parameters, above the infrasonic spectrum, that applied uniformly to human osteoblast-like cells in monolayer culture, aiming to identify a range of mechanical parameters that are effective to enhance osteoblast proliferation in vitro. Methods: Human osteoblast-like cells in explant monolayer culture samples were exposed to mechanical vibration in the 10-70Hz range of frequencies for two minutes, in four 24 hours intervals. Cell numbers in culture, cellular alkaline phosphatase activity (a marker of cell maturation), and lactate dehydrogenase activity in culture media (representing cell death) were measured after the mechanical stimulation protocol application and compared statistically to the control cell cultures kept in static conditions. The cell proliferation was deduced from cell number in culture and cell death measurements. Results: We found that 50-70 Hz of vibration frequency protocol (10-30 μm of maximal displacement amplitude, 0.03g of peak-to-peak acceleration) is optimal for enhancing cells' proliferation(p<0.05), with a parallel decrease of their maturation (p<0.01). Discussion: We detected the optimal mechanical parameters of excitation protocol for induction of osteoblast proliferation in vitro by a mechanical platform, which can be used as a standardized method in the research of mechanotransduction in human osteoblast.Nahum RosenbergOrit RosenbergJacob Halevi PolitchHaim AbramovichEmergency Department of Hospital San Pedro (Logroño, Spain)articleosteoblastmechanical stimulationvibrationmechanotransductionMedicine (General)R5-920ENIberoamerican Journal of Medicine, Vol 3, Iss 3, Pp 204-211 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	osteoblast mechanical stimulation vibration mechanotransduction Medicine (General) R5-920
spellingShingle	osteoblast mechanical stimulation vibration mechanotransduction Medicine (General) R5-920 Nahum Rosenberg Orit Rosenberg Jacob Halevi Politch Haim Abramovich Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration
description	Introduction: Biomechanical stimulation of cultured human osteoblast-like cells, which is based on controlled mechanical vibration, has been previously indicated, but the exact mechanical parameters that are effective for cells' proliferation enhancement are still elusive due to the lack of direct data recordings from the stimulated cells in culture. Therefore, we developed a low friction tunable system that enables recording of a narrow range of mechanical parameters, above the infrasonic spectrum, that applied uniformly to human osteoblast-like cells in monolayer culture, aiming to identify a range of mechanical parameters that are effective to enhance osteoblast proliferation in vitro. Methods: Human osteoblast-like cells in explant monolayer culture samples were exposed to mechanical vibration in the 10-70Hz range of frequencies for two minutes, in four 24 hours intervals. Cell numbers in culture, cellular alkaline phosphatase activity (a marker of cell maturation), and lactate dehydrogenase activity in culture media (representing cell death) were measured after the mechanical stimulation protocol application and compared statistically to the control cell cultures kept in static conditions. The cell proliferation was deduced from cell number in culture and cell death measurements. Results: We found that 50-70 Hz of vibration frequency protocol (10-30 μm of maximal displacement amplitude, 0.03g of peak-to-peak acceleration) is optimal for enhancing cells' proliferation(p<0.05), with a parallel decrease of their maturation (p<0.01). Discussion: We detected the optimal mechanical parameters of excitation protocol for induction of osteoblast proliferation in vitro by a mechanical platform, which can be used as a standardized method in the research of mechanotransduction in human osteoblast.
format	article
author	Nahum Rosenberg Orit Rosenberg Jacob Halevi Politch Haim Abramovich
author_facet	Nahum Rosenberg Orit Rosenberg Jacob Halevi Politch Haim Abramovich
author_sort	Nahum Rosenberg
title	Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration
title_short	Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration
title_full	Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration
title_fullStr	Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration
title_full_unstemmed	Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration
title_sort	optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration
publisher	Emergency Department of Hospital San Pedro (Logroño, Spain)
publishDate	2021
url	https://doi.org/10.5281/zenodo.4746404 https://doaj.org/article/081aec6b67d6443396c83b6a3bf07397
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Optimal parameters for the enhancement of human osteoblast-like cell proliferation in vitro via shear stress induced by high-frequency mechanical vibration

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