Force-velocity measurements of a few growing actin filaments.

The polymerization of actin in filaments generates forces that play a pivotal role in many cellular processes. We introduce a novel technique to determine the force-velocity relation when a few independent anchored filaments grow between magnetic colloidal particles. When a magnetic field is applied...

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Autores principales:	Coraline Brangbour, Olivia du Roure, Emmanuèle Helfer, Damien Démoulin, Alexis Mazurier, Marc Fermigier, Marie-France Carlier, Jérôme Bibette, Jean Baudry
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/082394e0811f45f49e6719676dac6865
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spelling	oai:doaj.org-article:082394e0811f45f49e6719676dac68652021-11-18T05:36:13ZForce-velocity measurements of a few growing actin filaments.1544-91731545-788510.1371/journal.pbio.1000613https://doaj.org/article/082394e0811f45f49e6719676dac68652011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21541364/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The polymerization of actin in filaments generates forces that play a pivotal role in many cellular processes. We introduce a novel technique to determine the force-velocity relation when a few independent anchored filaments grow between magnetic colloidal particles. When a magnetic field is applied, the colloidal particles assemble into chains under controlled loading or spacing. As the filaments elongate, the beads separate, allowing the force-velocity curve to be precisely measured. In the widely accepted Brownian ratchet model, the transduced force is associated with the slowing down of the on-rate polymerization. Unexpectedly, in our experiments, filaments are shown to grow at the same rate as when they are free in solution. However, as they elongate, filaments are more confined in the interspace between beads. Higher repulsive forces result from this higher confinement, which is associated with a lower entropy. In this mechanism, the production of force is not controlled by the polymerization rate, but is a consequence of the restriction of filaments' orientational fluctuations at their attachment point.Coraline BrangbourOlivia du RoureEmmanuèle HelferDamien DémoulinAlexis MazurierMarc FermigierMarie-France CarlierJérôme BibetteJean BaudryPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 9, Iss 4, p e1000613 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Coraline Brangbour Olivia du Roure Emmanuèle Helfer Damien Démoulin Alexis Mazurier Marc Fermigier Marie-France Carlier Jérôme Bibette Jean Baudry Force-velocity measurements of a few growing actin filaments.
description	The polymerization of actin in filaments generates forces that play a pivotal role in many cellular processes. We introduce a novel technique to determine the force-velocity relation when a few independent anchored filaments grow between magnetic colloidal particles. When a magnetic field is applied, the colloidal particles assemble into chains under controlled loading or spacing. As the filaments elongate, the beads separate, allowing the force-velocity curve to be precisely measured. In the widely accepted Brownian ratchet model, the transduced force is associated with the slowing down of the on-rate polymerization. Unexpectedly, in our experiments, filaments are shown to grow at the same rate as when they are free in solution. However, as they elongate, filaments are more confined in the interspace between beads. Higher repulsive forces result from this higher confinement, which is associated with a lower entropy. In this mechanism, the production of force is not controlled by the polymerization rate, but is a consequence of the restriction of filaments' orientational fluctuations at their attachment point.
format	article
author	Coraline Brangbour Olivia du Roure Emmanuèle Helfer Damien Démoulin Alexis Mazurier Marc Fermigier Marie-France Carlier Jérôme Bibette Jean Baudry
author_facet	Coraline Brangbour Olivia du Roure Emmanuèle Helfer Damien Démoulin Alexis Mazurier Marc Fermigier Marie-France Carlier Jérôme Bibette Jean Baudry
author_sort	Coraline Brangbour
title	Force-velocity measurements of a few growing actin filaments.
title_short	Force-velocity measurements of a few growing actin filaments.
title_full	Force-velocity measurements of a few growing actin filaments.
title_fullStr	Force-velocity measurements of a few growing actin filaments.
title_full_unstemmed	Force-velocity measurements of a few growing actin filaments.
title_sort	force-velocity measurements of a few growing actin filaments.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/082394e0811f45f49e6719676dac6865
work_keys_str_mv	AT coralinebrangbour forcevelocitymeasurementsofafewgrowingactinfilaments AT oliviaduroure forcevelocitymeasurementsofafewgrowingactinfilaments AT emmanuelehelfer forcevelocitymeasurementsofafewgrowingactinfilaments AT damiendemoulin forcevelocitymeasurementsofafewgrowingactinfilaments AT alexismazurier forcevelocitymeasurementsofafewgrowingactinfilaments AT marcfermigier forcevelocitymeasurementsofafewgrowingactinfilaments AT mariefrancecarlier forcevelocitymeasurementsofafewgrowingactinfilaments AT jeromebibette forcevelocitymeasurementsofafewgrowingactinfilaments AT jeanbaudry forcevelocitymeasurementsofafewgrowingactinfilaments
_version_	1718424893043769344

Force-velocity measurements of a few growing actin filaments.

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