Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis

Chunhua Wu,1 Mengmeng Li,2 Wenji Chen1 1Department of General Practice, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, People’s Republic of China; 2Department of Ophthalmology, Xuzhou First People’s Hospital, Xuzhou 221000, People’s Republic of...

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Autores principales:	Wu C, Li M, Chen W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	chronic pancreatitis cerulein gut microbiota body weight weight Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/0826cb33bcab4fc98135418f05340f3b
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spelling	oai:doaj.org-article:0826cb33bcab4fc98135418f05340f3b2021-12-02T13:53:35ZCharacteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis1178-7007https://doaj.org/article/0826cb33bcab4fc98135418f05340f3b2021-01-01T00:00:00Zhttps://www.dovepress.com/characteristics-of-gut-microbiota-in-cerulein-induced-chronic-pancreat-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Chunhua Wu,1 Mengmeng Li,2 Wenji Chen1 1Department of General Practice, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, People’s Republic of China; 2Department of Ophthalmology, Xuzhou First People’s Hospital, Xuzhou 221000, People’s Republic of ChinaCorrespondence: Wenji Chen Email chwenji@163.comBackground: Although clinical trials and animal models have evaluated the alterations of the microbiome in chronic pancreatitis (CP), the gut microbiota composition and diversity in cerulein-induced CP is unknown. This study aimed to evaluate the changes of gut microbiota in a CP mice model, and to determine whether these gut microbiota changes were consistent with those in patients with CP.Methods: A total of ten male C57BL/6j mice were randomly divided into two groups. The experimental group were injected intraperitoneally with cerulein, while the normal control group received comparable injections of saline, the entire molding process lasted 6 weeks. Histology analysis was used to assess pancreatic morphological changes and fibrosis, meanwhile the gut microbiota composition and diversity were analyzed by high throughput sequencing. Spearman correlation analysis was used to determine whether body weight and weight changes were associated with changes in gut microbial abundance.Results: The bacterial richness and diversity of CP mice decreased, and the gut microbiota changed, including lower levels of Firmicutes, decreased Firmicutes/Bacteroidetes ratio and increased abundance of Bacteroidetes, Actinobacteria and Verrucomicrobia. We found statistically significant differences in body weight and weight changes between the two groups. However, there was no significant correlation between alterations of gut microbiota and in body weight and weight changes.Conclusion: Our results showed that the gut microbiota in cerulein-induced CP was changed.Keywords: chronic pancreatitis, cerulein, gut microbiota, body weight, weight changesWu CLi MChen WDove Medical Pressarticlechronic pancreatitisceruleingut microbiotabody weightweightSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 285-294 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic pancreatitis cerulein gut microbiota body weight weight Specialties of internal medicine RC581-951
spellingShingle	chronic pancreatitis cerulein gut microbiota body weight weight Specialties of internal medicine RC581-951 Wu C Li M Chen W Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis
description	Chunhua Wu,1 Mengmeng Li,2 Wenji Chen1 1Department of General Practice, Zhongda Hospital, Medical School, Southeast University, Nanjing 210009, People’s Republic of China; 2Department of Ophthalmology, Xuzhou First People’s Hospital, Xuzhou 221000, People’s Republic of ChinaCorrespondence: Wenji Chen Email chwenji@163.comBackground: Although clinical trials and animal models have evaluated the alterations of the microbiome in chronic pancreatitis (CP), the gut microbiota composition and diversity in cerulein-induced CP is unknown. This study aimed to evaluate the changes of gut microbiota in a CP mice model, and to determine whether these gut microbiota changes were consistent with those in patients with CP.Methods: A total of ten male C57BL/6j mice were randomly divided into two groups. The experimental group were injected intraperitoneally with cerulein, while the normal control group received comparable injections of saline, the entire molding process lasted 6 weeks. Histology analysis was used to assess pancreatic morphological changes and fibrosis, meanwhile the gut microbiota composition and diversity were analyzed by high throughput sequencing. Spearman correlation analysis was used to determine whether body weight and weight changes were associated with changes in gut microbial abundance.Results: The bacterial richness and diversity of CP mice decreased, and the gut microbiota changed, including lower levels of Firmicutes, decreased Firmicutes/Bacteroidetes ratio and increased abundance of Bacteroidetes, Actinobacteria and Verrucomicrobia. We found statistically significant differences in body weight and weight changes between the two groups. However, there was no significant correlation between alterations of gut microbiota and in body weight and weight changes.Conclusion: Our results showed that the gut microbiota in cerulein-induced CP was changed.Keywords: chronic pancreatitis, cerulein, gut microbiota, body weight, weight changes
format	article
author	Wu C Li M Chen W
author_facet	Wu C Li M Chen W
author_sort	Wu C
title	Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis
title_short	Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis
title_full	Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis
title_fullStr	Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis
title_full_unstemmed	Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis
title_sort	characteristics of gut microbiota in cerulein-induced chronic pancreatitis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/0826cb33bcab4fc98135418f05340f3b
work_keys_str_mv	AT wuc characteristicsofgutmicrobiotainceruleininducedchronicpancreatitis AT lim characteristicsofgutmicrobiotainceruleininducedchronicpancreatitis AT chenw characteristicsofgutmicrobiotainceruleininducedchronicpancreatitis
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Characteristics of Gut Microbiota in Cerulein-Induced Chronic Pancreatitis

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