Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis

Marlena Zielinska1,2, Ewa Sawosz1, Marta Grodzik1, Mateusz Wierzbicki1, Maria Gromadka1, Anna Hotowy3, Filip Sawosz3, Andrzej Lozicki1, Andr&egrave; Chwalibog31Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life sciences, Warsaw, 2The Kielanowski Institute of A...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Zielinska M, Sawosz E, Grodzik M, Wierzbicki M, Gromadka M, Hotowy A, Sawosz F, Lozicki A, Chwalibog A
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/08281938c7e44d3c9a98699908a207c6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:08281938c7e44d3c9a98699908a207c6
record_format	dspace
spelling	oai:doaj.org-article:08281938c7e44d3c9a98699908a207c62021-12-02T05:40:40ZEffect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis1176-91141178-2013https://doaj.org/article/08281938c7e44d3c9a98699908a207c62011-12-01T00:00:00Zhttp://www.dovepress.com/effect-of-heparan-sulfate-and-gold-nanoparticles-on-muscle-development-a8802https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Marlena Zielinska1,2, Ewa Sawosz1, Marta Grodzik1, Mateusz Wierzbicki1, Maria Gromadka1, Anna Hotowy3, Filip Sawosz3, Andrzej Lozicki1, Andr&egrave; Chwalibog31Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life sciences, Warsaw, 2The Kielanowski Institute of Animal Physiology and Nutrition, Jablonna, Poland; 3Department of Basic Animal and Veterinary sciences, University of copenhagen, Frederiksberg, DenmarkPurpose: It was hypothesized that heparan sulfate (HS) as an essential compound for myogenesis and nanoparticles of gold (nano-Au) as highly reactive compounds can affect muscle development as a consequence of molecular regulation of muscle cell formation, and that these effects may be enhanced by a complex of HS conjugated with nano-Au. The objective of the present study was to determine the effect of administration of nano-Au, HS, and a nano-Au+HS complex on the morphological and molecular characteristics of breast muscle during embryogenesis.Methods: Chicken embryos were used as in vivo model. Fertilized chicken eggs (n = 350) were randomly divided into the control group and the groups treated with nano-Au, HS, and nano-Au+HS. The experimental solutions were given in ovo on the first day of incubation and the embryos were evaluated on day 20 of incubation. The methods included biochemical indi- ces in blood, immunohistochemistry, microscopy (transmission electron microscopy, scanning electron microscopy, confocal), and gene expression at the messenger ribonucleic acid and protein levels.Results: The treatments did not adversely affect mortality, organ weight, and homeostasis of the embryos. HS stimulated the development and maturation of breast muscle by increasing the number of nuclei, satellite cells, and muscle fibers and affected the expression of basic fibroblast growth factor-2 and paired-box transcription factor-7. Furthermore, the nano-Au+HS complex contributed to the increased number of myocytes and nuclei in chicken embryo muscles.Conclusion: The results indicate that the administration of HS and nano-Au affects muscle development and that this effect is enhanced by conjugating HS with nano-Au.Keywords: gold nanoparticles, heparan sulfate, muscle development, embryogenesis, chickenZielinska MSawosz EGrodzik MWierzbicki MGromadka MHotowy ASawosz FLozicki AChwalibog ADove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3163-3172 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zielinska M Sawosz E Grodzik M Wierzbicki M Gromadka M Hotowy A Sawosz F Lozicki A Chwalibog A Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis
description	Marlena Zielinska1,2, Ewa Sawosz1, Marta Grodzik1, Mateusz Wierzbicki1, Maria Gromadka1, Anna Hotowy3, Filip Sawosz3, Andrzej Lozicki1, Andr&egrave; Chwalibog31Division of Biotechnology and Biochemistry of Nutrition, Warsaw University of Life sciences, Warsaw, 2The Kielanowski Institute of Animal Physiology and Nutrition, Jablonna, Poland; 3Department of Basic Animal and Veterinary sciences, University of copenhagen, Frederiksberg, DenmarkPurpose: It was hypothesized that heparan sulfate (HS) as an essential compound for myogenesis and nanoparticles of gold (nano-Au) as highly reactive compounds can affect muscle development as a consequence of molecular regulation of muscle cell formation, and that these effects may be enhanced by a complex of HS conjugated with nano-Au. The objective of the present study was to determine the effect of administration of nano-Au, HS, and a nano-Au+HS complex on the morphological and molecular characteristics of breast muscle during embryogenesis.Methods: Chicken embryos were used as in vivo model. Fertilized chicken eggs (n = 350) were randomly divided into the control group and the groups treated with nano-Au, HS, and nano-Au+HS. The experimental solutions were given in ovo on the first day of incubation and the embryos were evaluated on day 20 of incubation. The methods included biochemical indi- ces in blood, immunohistochemistry, microscopy (transmission electron microscopy, scanning electron microscopy, confocal), and gene expression at the messenger ribonucleic acid and protein levels.Results: The treatments did not adversely affect mortality, organ weight, and homeostasis of the embryos. HS stimulated the development and maturation of breast muscle by increasing the number of nuclei, satellite cells, and muscle fibers and affected the expression of basic fibroblast growth factor-2 and paired-box transcription factor-7. Furthermore, the nano-Au+HS complex contributed to the increased number of myocytes and nuclei in chicken embryo muscles.Conclusion: The results indicate that the administration of HS and nano-Au affects muscle development and that this effect is enhanced by conjugating HS with nano-Au.Keywords: gold nanoparticles, heparan sulfate, muscle development, embryogenesis, chicken
format	article
author	Zielinska M Sawosz E Grodzik M Wierzbicki M Gromadka M Hotowy A Sawosz F Lozicki A Chwalibog A
author_facet	Zielinska M Sawosz E Grodzik M Wierzbicki M Gromadka M Hotowy A Sawosz F Lozicki A Chwalibog A
author_sort	Zielinska M
title	Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis
title_short	Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis
title_full	Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis
title_fullStr	Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis
title_full_unstemmed	Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis
title_sort	effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/08281938c7e44d3c9a98699908a207c6
work_keys_str_mv	AT zielinskam effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT sawosze effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT grodzikm effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT wierzbickim effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT gromadkam effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT hotowya effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT sawoszf effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT lozickia effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis AT chwaliboga effectofheparansulfateandgoldnanoparticlesonmuscledevelopmentduringembryogenesis
_version_	1718400273480679424

Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis

Ejemplares similares