Metabolomics study of fibroblasts damaged by UVB and BaP

Abstract We have recently shown that both UVB and BaP can induce the production of ROS, apoptosis and even cancer. However, the differences in the metabolic profiles of skin damaged by UVB, BaP or UVB combined with BaP have not been studied. Therefore, we examined the metabolic changes in the human...

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Autores principales:	Xiaoyu Yang, Jiateng Wang, Hecong Wang, Xueying Li, Congfen He, Lei Liu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/082892cd81e445ac8703c444149c2493
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spelling	oai:doaj.org-article:082892cd81e445ac8703c444149c24932021-12-02T14:49:24ZMetabolomics study of fibroblasts damaged by UVB and BaP10.1038/s41598-021-90186-72045-2322https://doaj.org/article/082892cd81e445ac8703c444149c24932021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90186-7https://doaj.org/toc/2045-2322Abstract We have recently shown that both UVB and BaP can induce the production of ROS, apoptosis and even cancer. However, the differences in the metabolic profiles of skin damaged by UVB, BaP or UVB combined with BaP have not been studied. Therefore, we examined the metabolic changes in the human foreskin fibroblast injured by UVB or BaP or the combination of the two, using ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (qTOF-MS). 24 metabolites were altered in the UVB damage group, 25 in the BaP damage group, and 33 in the UVB combined with BaP group. These alterations indicated that the metabolic mechanisms of HFF-1 cells treated with UVB or BaP are related to multiple main metabolites including glycerophosphocholine (PC), lactosylceramide (LacCer), guanidinosuccinic acid (GSA), glutathione(GSH), and lysophosphatidylcholine (LysoPC) and the main mechanisms involved glycerophospholipid and glutathione metabolism. Thus, our report provided useful insight into the underlying mechanisms of UVB and BaP damage to skin cells.Xiaoyu YangJiateng WangHecong WangXueying LiCongfen HeLei LiuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoyu Yang Jiateng Wang Hecong Wang Xueying Li Congfen He Lei Liu Metabolomics study of fibroblasts damaged by UVB and BaP
description	Abstract We have recently shown that both UVB and BaP can induce the production of ROS, apoptosis and even cancer. However, the differences in the metabolic profiles of skin damaged by UVB, BaP or UVB combined with BaP have not been studied. Therefore, we examined the metabolic changes in the human foreskin fibroblast injured by UVB or BaP or the combination of the two, using ultra performance liquid chromatography (UPLC) coupled with quadrupole time-of-flight mass spectrometry (qTOF-MS). 24 metabolites were altered in the UVB damage group, 25 in the BaP damage group, and 33 in the UVB combined with BaP group. These alterations indicated that the metabolic mechanisms of HFF-1 cells treated with UVB or BaP are related to multiple main metabolites including glycerophosphocholine (PC), lactosylceramide (LacCer), guanidinosuccinic acid (GSA), glutathione(GSH), and lysophosphatidylcholine (LysoPC) and the main mechanisms involved glycerophospholipid and glutathione metabolism. Thus, our report provided useful insight into the underlying mechanisms of UVB and BaP damage to skin cells.
format	article
author	Xiaoyu Yang Jiateng Wang Hecong Wang Xueying Li Congfen He Lei Liu
author_facet	Xiaoyu Yang Jiateng Wang Hecong Wang Xueying Li Congfen He Lei Liu
author_sort	Xiaoyu Yang
title	Metabolomics study of fibroblasts damaged by UVB and BaP
title_short	Metabolomics study of fibroblasts damaged by UVB and BaP
title_full	Metabolomics study of fibroblasts damaged by UVB and BaP
title_fullStr	Metabolomics study of fibroblasts damaged by UVB and BaP
title_full_unstemmed	Metabolomics study of fibroblasts damaged by UVB and BaP
title_sort	metabolomics study of fibroblasts damaged by uvb and bap
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/082892cd81e445ac8703c444149c2493
work_keys_str_mv	AT xiaoyuyang metabolomicsstudyoffibroblastsdamagedbyuvbandbap AT jiatengwang metabolomicsstudyoffibroblastsdamagedbyuvbandbap AT hecongwang metabolomicsstudyoffibroblastsdamagedbyuvbandbap AT xueyingli metabolomicsstudyoffibroblastsdamagedbyuvbandbap AT congfenhe metabolomicsstudyoffibroblastsdamagedbyuvbandbap AT leiliu metabolomicsstudyoffibroblastsdamagedbyuvbandbap
_version_	1718389490320408576

Metabolomics study of fibroblasts damaged by UVB and BaP

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