Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow

Medical instruments are generally sterilized using high-pressure steam, ethylene oxide gas, gamma radiation, or electron beams. However, such conventional methods entail many shortcomings such as limitations of applicable materials, high temperature, long time, and high operating costs. Therefore, d...

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Autores principales:	Daisuke YOSHINO, Kazuhiro NAKAMURAYA, Tomoki NAKAJIMA, Takehiko SATO
Formato:	article
Lenguaje:	EN
Publicado:	The Japan Society of Mechanical Engineers 2015
Materias:	atmospheric-pressure plasma low-temperature plasma surface discharge sterilization circulating flow nitrogen oxide Mechanical engineering and machinery TJ1-1570
Acceso en línea:	https://doaj.org/article/082cef21462c430fa8a42608e731e0ad
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spelling	oai:doaj.org-article:082cef21462c430fa8a42608e731e0ad2021-11-26T06:30:10ZDevelopment of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow2187-974510.1299/mej.15-00187https://doaj.org/article/082cef21462c430fa8a42608e731e0ad2015-09-01T00:00:00Zhttps://www.jstage.jst.go.jp/article/mej/2/5/2_15-00187/_pdf/-char/enhttps://doaj.org/toc/2187-9745Medical instruments are generally sterilized using high-pressure steam, ethylene oxide gas, gamma radiation, or electron beams. However, such conventional methods entail many shortcomings such as limitations of applicable materials, high temperature, long time, and high operating costs. Therefore, development of a safe, low-temperature, rapid, and inexpensive sterilization method has been anticipated. This study developed and assessed a plasma sterilization device that can generate effective chemical species necessary for sterilization. Its sterilization effectiveness was verified using biological indicators of Geobacillus stearothermophilus and Bacillus atrophaeus spores. The G. stearothermophilus and B. atrophaeus were sterilized, respectively, in 25 and 35 min with 5,400 ppm and in 35 min with 7,600 ppm of plasma-generated NOx, respectively, at around 25 °C. The main factor in sterilization of spores using the plasma was also assessed. In the conditions of atmospheric pressure air plasma, nitrogen oxide such as NO and NO2 were mainly generated. Morphological observations of spores exposed to the plasma and NO2 using scanning electron microscopy confirmed that nitrogen oxide perforated the spore coats. That spore coat perforation caused by nitrogen oxide is expected to be a main factor in sterilization. To remove the toxic gases used for sterilization, two types of methods were introduced into the developed devices. Bubbling in water could be reduced the concentration of residual NOx in the reaction chamber after sterilization to less than 50 ppm, but it remained higher than the limitation of environmental regulations. On the other hand, using the surface discharge system, we were able to reduce the residual NOx to less than 0.04 ppm, which is the limit of environmental regulations. This study presents the important techniques to improve infection prevention and public health.Daisuke YOSHINOKazuhiro NAKAMURAYATomoki NAKAJIMATakehiko SATOThe Japan Society of Mechanical Engineersarticleatmospheric-pressure plasmalow-temperature plasmasurface dischargesterilizationcirculating flownitrogen oxideMechanical engineering and machineryTJ1-1570ENMechanical Engineering Journal, Vol 2, Iss 5, Pp 15-00187-15-00187 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	atmospheric-pressure plasma low-temperature plasma surface discharge sterilization circulating flow nitrogen oxide Mechanical engineering and machinery TJ1-1570
spellingShingle	atmospheric-pressure plasma low-temperature plasma surface discharge sterilization circulating flow nitrogen oxide Mechanical engineering and machinery TJ1-1570 Daisuke YOSHINO Kazuhiro NAKAMURAYA Tomoki NAKAJIMA Takehiko SATO Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow
description	Medical instruments are generally sterilized using high-pressure steam, ethylene oxide gas, gamma radiation, or electron beams. However, such conventional methods entail many shortcomings such as limitations of applicable materials, high temperature, long time, and high operating costs. Therefore, development of a safe, low-temperature, rapid, and inexpensive sterilization method has been anticipated. This study developed and assessed a plasma sterilization device that can generate effective chemical species necessary for sterilization. Its sterilization effectiveness was verified using biological indicators of Geobacillus stearothermophilus and Bacillus atrophaeus spores. The G. stearothermophilus and B. atrophaeus were sterilized, respectively, in 25 and 35 min with 5,400 ppm and in 35 min with 7,600 ppm of plasma-generated NOx, respectively, at around 25 °C. The main factor in sterilization of spores using the plasma was also assessed. In the conditions of atmospheric pressure air plasma, nitrogen oxide such as NO and NO2 were mainly generated. Morphological observations of spores exposed to the plasma and NO2 using scanning electron microscopy confirmed that nitrogen oxide perforated the spore coats. That spore coat perforation caused by nitrogen oxide is expected to be a main factor in sterilization. To remove the toxic gases used for sterilization, two types of methods were introduced into the developed devices. Bubbling in water could be reduced the concentration of residual NOx in the reaction chamber after sterilization to less than 50 ppm, but it remained higher than the limitation of environmental regulations. On the other hand, using the surface discharge system, we were able to reduce the residual NOx to less than 0.04 ppm, which is the limit of environmental regulations. This study presents the important techniques to improve infection prevention and public health.
format	article
author	Daisuke YOSHINO Kazuhiro NAKAMURAYA Tomoki NAKAJIMA Takehiko SATO
author_facet	Daisuke YOSHINO Kazuhiro NAKAMURAYA Tomoki NAKAJIMA Takehiko SATO
author_sort	Daisuke YOSHINO
title	Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow
title_short	Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow
title_full	Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow
title_fullStr	Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow
title_full_unstemmed	Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow
title_sort	development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow
publisher	The Japan Society of Mechanical Engineers
publishDate	2015
url	https://doaj.org/article/082cef21462c430fa8a42608e731e0ad
work_keys_str_mv	AT daisukeyoshino developmentoflowtemperaturesterilizationdeviceusingatmosphericpressureairplasmawithcirculatingflow AT kazuhironakamuraya developmentoflowtemperaturesterilizationdeviceusingatmosphericpressureairplasmawithcirculatingflow AT tomokinakajima developmentoflowtemperaturesterilizationdeviceusingatmosphericpressureairplasmawithcirculatingflow AT takehikosato developmentoflowtemperaturesterilizationdeviceusingatmosphericpressureairplasmawithcirculatingflow
_version_	1718409759991791616

Development of low-temperature sterilization device using atmospheric pressure air plasma with circulating flow

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