The Impact of the Entomopathogenic Fungus Conidiobolus coronatus on the Free Fatty Acid Profile of the Flesh Fly Sarcophaga argyrostoma

The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus Conidiobolus coronatus. In this study, Sarcophaga argy...

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Autores principales:	Agata Kaczmarek, Mieczysława Irena Boguś
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cuticle entomopathogens free fatty acids fungal infection polyunsaturated fatty acids Science Q
Acceso en línea:	https://doaj.org/article/083ebd7306494843bf3026cb0426c03a
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spelling	oai:doaj.org-article:083ebd7306494843bf3026cb0426c03a2021-11-25T17:59:10ZThe Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>10.3390/insects121109702075-4450https://doaj.org/article/083ebd7306494843bf3026cb0426c03a2021-10-01T00:00:00Zhttps://www.mdpi.com/2075-4450/12/11/970https://doaj.org/toc/2075-4450The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus <i>Conidiobolus coronatus</i>. In this study, <i>Sarcophaga argyrostoma</i> flies were exposed to sporulating <i>C. coronatus</i> colonies for 24 h; the pupae were resistant, but the adults demonstrated 60% mortality. Although the pupae demonstrated no sign of infection nor any abnormal development, our findings indicate that after 24 h of contact with the fungus, the pupae demonstrated a 25.2-fold increase in total cuticular free fatty acids (FFAs) and a 1.9-fold decrease in total internal FFAs. Also, the cuticular FFA increased from 26 to 30, while the internal FFA class increased from 13 to 23. In exposed adults, the total mass of cuticular FFAs increased 1.7-fold, while the number of FFAs stayed the same (32 FFAs). Also, the internal FFA class increased from 26 to 35 and the total FFA mass increased 1.1-fold. These considerable differences between adults and pupae associated with <i>C. coronatus</i> exposure indicate developmental changes in the mechanisms governing lipid metabolism and spatial distribution in the organism, and suggest that cuticular lipids play a vital role in the defence against pathogenic fungi.Agata KaczmarekMieczysława Irena BoguśMDPI AGarticlecuticleentomopathogensfree fatty acidsfungal infectionpolyunsaturated fatty acidsScienceQENInsects, Vol 12, Iss 970, p 970 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cuticle entomopathogens free fatty acids fungal infection polyunsaturated fatty acids Science Q
spellingShingle	cuticle entomopathogens free fatty acids fungal infection polyunsaturated fatty acids Science Q Agata Kaczmarek Mieczysława Irena Boguś The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>
description	The chemical composition of the insect cuticle varies remarkably between species and their life stages. It can affect host resistance and substrate utilization by invading entomopathogen fungi, such as the soil fungus <i>Conidiobolus coronatus</i>. In this study, <i>Sarcophaga argyrostoma</i> flies were exposed to sporulating <i>C. coronatus</i> colonies for 24 h; the pupae were resistant, but the adults demonstrated 60% mortality. Although the pupae demonstrated no sign of infection nor any abnormal development, our findings indicate that after 24 h of contact with the fungus, the pupae demonstrated a 25.2-fold increase in total cuticular free fatty acids (FFAs) and a 1.9-fold decrease in total internal FFAs. Also, the cuticular FFA increased from 26 to 30, while the internal FFA class increased from 13 to 23. In exposed adults, the total mass of cuticular FFAs increased 1.7-fold, while the number of FFAs stayed the same (32 FFAs). Also, the internal FFA class increased from 26 to 35 and the total FFA mass increased 1.1-fold. These considerable differences between adults and pupae associated with <i>C. coronatus</i> exposure indicate developmental changes in the mechanisms governing lipid metabolism and spatial distribution in the organism, and suggest that cuticular lipids play a vital role in the defence against pathogenic fungi.
format	article
author	Agata Kaczmarek Mieczysława Irena Boguś
author_facet	Agata Kaczmarek Mieczysława Irena Boguś
author_sort	Agata Kaczmarek
title	The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>
title_short	The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>
title_full	The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>
title_fullStr	The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>
title_full_unstemmed	The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>
title_sort	impact of the entomopathogenic fungus <i>conidiobolus coronatus</i> on the free fatty acid profile of the flesh fly <i>sarcophaga argyrostoma</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/083ebd7306494843bf3026cb0426c03a
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The Impact of the Entomopathogenic Fungus <i>Conidiobolus coronatus</i> on the Free Fatty Acid Profile of the Flesh Fly <i>Sarcophaga argyrostoma</i>

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