Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7

Introduction: Transforming growth factor-β (TGF-β), a common outcome of various progressive chronic kidney diseases, can regulate and induce fibrosis. Objective: The study aimed to identify downstream targets of lncRNA ENST00000453774.1 (lnc453774.1) and outline their functions on the development of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiangning Yuan, Wen-bin Tang, Ling Peng, Yusa Chen, Shumei Tang, Huipeng Ge, Xiufen Wang, Xiangcheng Xiao
Formato:	article
Lenguaje:	EN
Publicado:	Karger Publishers 2021
Materias:	renal fibrosis long noncoding rna enst00000453774.1 transforming growth factor-β1 weighted gene correlation network analysis rna sequencing Dermatology RL1-803 Diseases of the circulatory (Cardiovascular) system RC666-701 Diseases of the genitourinary system. Urology RC870-923
Acceso en línea:	https://doaj.org/article/0845dde470144c9592a4a43dfd5ed87e
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0845dde470144c9592a4a43dfd5ed87e
record_format	dspace
spelling	oai:doaj.org-article:0845dde470144c9592a4a43dfd5ed87e2021-11-04T14:40:31ZElevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF71420-40961423-014310.1159/000515624https://doaj.org/article/0845dde470144c9592a4a43dfd5ed87e2021-10-01T00:00:00Zhttps://www.karger.com/Article/FullText/515624https://doaj.org/toc/1420-4096https://doaj.org/toc/1423-0143Introduction: Transforming growth factor-β (TGF-β), a common outcome of various progressive chronic kidney diseases, can regulate and induce fibrosis. Objective: The study aimed to identify downstream targets of lncRNA ENST00000453774.1 (lnc453774.1) and outline their functions on the development of renal fibrosis. Methods: HK-2 cells were induced with 5 ng/mL TGF-β1 for 24 h to construct a renal fibrosis cell model. Differentially expressed genes (DEGs) targeted by lnc453774.1 in TGF-β1-induced renal fibrosis were identified using RNA sequencing. The dataset GSE23338 was employed to identify DEGs in 48-h TGF-β1-stimulated human kidney epithelial cells, and these DEGs were intersected with genes in the key module using weighted gene co-expression network analysis to generate key genes associated with renal fibrosis. MicroRNAs (miRs) that had targeting relationship with keys genes and lnc453774.1 were predicted by using Miranda software, and important genes were intersected with key genes that had targeting relationship with these miRs. Key target genes by lnc453774.1 were identified in a protein-protein interaction network among lnc453774.1, important genes, and reported genes related to autophagy, oxidative stress, and cell adhesion. Results: Key genes in the key module (turquoise) were intersected with DEGs in the dataset GSE23338 and yielded 20 key genes regulated by lnc453774.1 involved in renal fibrosis. Fourteen miRs had targeting relationship with lnc453774.1 and key genes, and 8 important genes targeted by these 14 miRs were identified. Fibrillin-1 (FBN1), insulin-like growth factor 1 receptor (IGF1R), and Kruppel-like factor 7 (KLF7) were identified to be involved in autophagy, oxidative stress, and cell adhesion and were elevated in the lnc453774.1-overexpressing TGF-β1-induced cells. Conclusion: These results show FBN1, IGF1R, and KLF7 serve as downstream targets of lnc453774.1, and that lnc453774.1 may protect against renal fibrosis through competing endogenous miRs which target FBN1, IGF1R, and KLF7 mRNAs.Xiangning YuanWen-bin TangLing PengYusa ChenShumei TangHuipeng GeXiufen WangXiangcheng XiaoKarger Publishersarticlerenal fibrosislong noncoding rna enst00000453774.1transforming growth factor-β1weighted gene correlation network analysisrna sequencingDermatologyRL1-803Diseases of the circulatory (Cardiovascular) systemRC666-701Diseases of the genitourinary system. UrologyRC870-923ENKidney & Blood Pressure Research, Vol 46, Iss 5, Pp 563-573 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	renal fibrosis long noncoding rna enst00000453774.1 transforming growth factor-β1 weighted gene correlation network analysis rna sequencing Dermatology RL1-803 Diseases of the circulatory (Cardiovascular) system RC666-701 Diseases of the genitourinary system. Urology RC870-923
spellingShingle	renal fibrosis long noncoding rna enst00000453774.1 transforming growth factor-β1 weighted gene correlation network analysis rna sequencing Dermatology RL1-803 Diseases of the circulatory (Cardiovascular) system RC666-701 Diseases of the genitourinary system. Urology RC870-923 Xiangning Yuan Wen-bin Tang Ling Peng Yusa Chen Shumei Tang Huipeng Ge Xiufen Wang Xiangcheng Xiao Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7
description	Introduction: Transforming growth factor-β (TGF-β), a common outcome of various progressive chronic kidney diseases, can regulate and induce fibrosis. Objective: The study aimed to identify downstream targets of lncRNA ENST00000453774.1 (lnc453774.1) and outline their functions on the development of renal fibrosis. Methods: HK-2 cells were induced with 5 ng/mL TGF-β1 for 24 h to construct a renal fibrosis cell model. Differentially expressed genes (DEGs) targeted by lnc453774.1 in TGF-β1-induced renal fibrosis were identified using RNA sequencing. The dataset GSE23338 was employed to identify DEGs in 48-h TGF-β1-stimulated human kidney epithelial cells, and these DEGs were intersected with genes in the key module using weighted gene co-expression network analysis to generate key genes associated with renal fibrosis. MicroRNAs (miRs) that had targeting relationship with keys genes and lnc453774.1 were predicted by using Miranda software, and important genes were intersected with key genes that had targeting relationship with these miRs. Key target genes by lnc453774.1 were identified in a protein-protein interaction network among lnc453774.1, important genes, and reported genes related to autophagy, oxidative stress, and cell adhesion. Results: Key genes in the key module (turquoise) were intersected with DEGs in the dataset GSE23338 and yielded 20 key genes regulated by lnc453774.1 involved in renal fibrosis. Fourteen miRs had targeting relationship with lnc453774.1 and key genes, and 8 important genes targeted by these 14 miRs were identified. Fibrillin-1 (FBN1), insulin-like growth factor 1 receptor (IGF1R), and Kruppel-like factor 7 (KLF7) were identified to be involved in autophagy, oxidative stress, and cell adhesion and were elevated in the lnc453774.1-overexpressing TGF-β1-induced cells. Conclusion: These results show FBN1, IGF1R, and KLF7 serve as downstream targets of lnc453774.1, and that lnc453774.1 may protect against renal fibrosis through competing endogenous miRs which target FBN1, IGF1R, and KLF7 mRNAs.
format	article
author	Xiangning Yuan Wen-bin Tang Ling Peng Yusa Chen Shumei Tang Huipeng Ge Xiufen Wang Xiangcheng Xiao
author_facet	Xiangning Yuan Wen-bin Tang Ling Peng Yusa Chen Shumei Tang Huipeng Ge Xiufen Wang Xiangcheng Xiao
author_sort	Xiangning Yuan
title	Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7
title_short	Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7
title_full	Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7
title_fullStr	Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7
title_full_unstemmed	Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7
title_sort	elevation of lncrna enst00000453774.1 prevents renal fibrosis by upregulating fbn1, igf1r, and klf7
publisher	Karger Publishers
publishDate	2021
url	https://doaj.org/article/0845dde470144c9592a4a43dfd5ed87e
work_keys_str_mv	AT xiangningyuan elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7 AT wenbintang elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7 AT lingpeng elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7 AT yusachen elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7 AT shumeitang elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7 AT huipengge elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7 AT xiufenwang elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7 AT xiangchengxiao elevationoflncrnaenst000004537741preventsrenalfibrosisbyupregulatingfbn1igf1randklf7
_version_	1718444817710579712

Elevation of LncRNA ENST00000453774.1 Prevents Renal Fibrosis by Upregulating FBN1, IGF1R, and KLF7

Ejemplares similares