Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.

Aspergillus flavus first gained scientific attention for its production of aflatoxin. The underlying regulation of aflatoxin biosynthesis has been serving as a theoretical model for biosynthesis of other microbial secondary metabolites. Nevertheless, for several decades, the DNA methylation status,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Si-Yang Liu, Jian-Qing Lin, Hong-Long Wu, Cheng-Cheng Wang, Shu-Jia Huang, Yan-Feng Luo, Ji-Hua Sun, Jian-Xiang Zhou, Shu-Jing Yan, Jian-Guo He, Jun Wang, Zhu-Mei He
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/0845f7014401473bbdd927a776d9a1d4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:0845f7014401473bbdd927a776d9a1d4
record_format	dspace
spelling	oai:doaj.org-article:0845f7014401473bbdd927a776d9a1d42021-11-18T07:29:43ZBisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.1932-620310.1371/journal.pone.0030349https://doaj.org/article/0845f7014401473bbdd927a776d9a1d42012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22276181/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Aspergillus flavus first gained scientific attention for its production of aflatoxin. The underlying regulation of aflatoxin biosynthesis has been serving as a theoretical model for biosynthesis of other microbial secondary metabolites. Nevertheless, for several decades, the DNA methylation status, one of the important epigenomic modifications involved in gene regulation, in A. flavus remains to be controversial. Here, we applied bisulfite sequencing in conjunction with a biological replicate strategy to investigate the DNA methylation profiling of A. flavus genome. Both the bisulfite sequencing data and the methylome comparisons with other fungi confirm that the DNA methylation level of this fungus is negligible. Further investigation into the DNA methyltransferase of Aspergillus uncovers its close relationship with RID-like enzymes as well as its divergence with the methyltransferase of species with validated DNA methylation. The lack of repeat contents of the A. flavus' genome and the high RIP-index of the small amount of remanent repeat potentially support our speculation that DNA methylation may be absent in A. flavus or that it may possess de novo DNA methylation which occurs very transiently during the obscure sexual stage of this fungal species. This work contributes to our understanding on the DNA methylation status of A. flavus, as well as reinforces our views on the DNA methylation in fungal species. In addition, our strategy of applying bisulfite sequencing to DNA methylation detection in species with low DNA methylation may serve as a reference for later scientific investigations in other hypomethylated species.Si-Yang LiuJian-Qing LinHong-Long WuCheng-Cheng WangShu-Jia HuangYan-Feng LuoJi-Hua SunJian-Xiang ZhouShu-Jing YanJian-Guo HeJun WangZhu-Mei HePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 1, p e30349 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Si-Yang Liu Jian-Qing Lin Hong-Long Wu Cheng-Cheng Wang Shu-Jia Huang Yan-Feng Luo Ji-Hua Sun Jian-Xiang Zhou Shu-Jing Yan Jian-Guo He Jun Wang Zhu-Mei He Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.
description	Aspergillus flavus first gained scientific attention for its production of aflatoxin. The underlying regulation of aflatoxin biosynthesis has been serving as a theoretical model for biosynthesis of other microbial secondary metabolites. Nevertheless, for several decades, the DNA methylation status, one of the important epigenomic modifications involved in gene regulation, in A. flavus remains to be controversial. Here, we applied bisulfite sequencing in conjunction with a biological replicate strategy to investigate the DNA methylation profiling of A. flavus genome. Both the bisulfite sequencing data and the methylome comparisons with other fungi confirm that the DNA methylation level of this fungus is negligible. Further investigation into the DNA methyltransferase of Aspergillus uncovers its close relationship with RID-like enzymes as well as its divergence with the methyltransferase of species with validated DNA methylation. The lack of repeat contents of the A. flavus' genome and the high RIP-index of the small amount of remanent repeat potentially support our speculation that DNA methylation may be absent in A. flavus or that it may possess de novo DNA methylation which occurs very transiently during the obscure sexual stage of this fungal species. This work contributes to our understanding on the DNA methylation status of A. flavus, as well as reinforces our views on the DNA methylation in fungal species. In addition, our strategy of applying bisulfite sequencing to DNA methylation detection in species with low DNA methylation may serve as a reference for later scientific investigations in other hypomethylated species.
format	article
author	Si-Yang Liu Jian-Qing Lin Hong-Long Wu Cheng-Cheng Wang Shu-Jia Huang Yan-Feng Luo Ji-Hua Sun Jian-Xiang Zhou Shu-Jing Yan Jian-Guo He Jun Wang Zhu-Mei He
author_facet	Si-Yang Liu Jian-Qing Lin Hong-Long Wu Cheng-Cheng Wang Shu-Jia Huang Yan-Feng Luo Ji-Hua Sun Jian-Xiang Zhou Shu-Jing Yan Jian-Guo He Jun Wang Zhu-Mei He
author_sort	Si-Yang Liu
title	Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.
title_short	Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.
title_full	Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.
title_fullStr	Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.
title_full_unstemmed	Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.
title_sort	bisulfite sequencing reveals that aspergillus flavus holds a hollow in dna methylation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/0845f7014401473bbdd927a776d9a1d4
work_keys_str_mv	AT siyangliu bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT jianqinglin bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT honglongwu bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT chengchengwang bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT shujiahuang bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT yanfengluo bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT jihuasun bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT jianxiangzhou bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT shujingyan bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT jianguohe bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT junwang bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation AT zhumeihe bisulfitesequencingrevealsthataspergillusflavusholdsahollowindnamethylation
_version_	1718423328763412480

Bisulfite sequencing reveals that Aspergillus flavus holds a hollow in DNA methylation.

Ejemplares similares