Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry

Abstract Accurate and precise determination of free fatty acid (FFA) contents is essential for quality control and assurance in food production. Herein, a mass spectrometric study was performed to develop a sample preparation protocol that can minimize exogenous FFA contamination during the quantifi...

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Autores principales:	Hyejin Park, Woo-Young Song, Hyeonjeon Cha, Tae-Young Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/085081ab168a4f82b7cda1ba79735a7c
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spelling	oai:doaj.org-article:085081ab168a4f82b7cda1ba79735a7c2021-12-02T16:31:07ZDevelopment of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry10.1038/s41598-021-85288-12045-2322https://doaj.org/article/085081ab168a4f82b7cda1ba79735a7c2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85288-1https://doaj.org/toc/2045-2322Abstract Accurate and precise determination of free fatty acid (FFA) contents is essential for quality control and assurance in food production. Herein, a mass spectrometric study was performed to develop a sample preparation protocol that can minimize exogenous FFA contamination during the quantification of FFA in food. The quantities of exogenous FFAs were measured using various combinations of seven pretreatment methods for a sample tube, three extraction methods, and four types of sample tubes. Methanol washing could effectively reduce exogenous palmitic acid (PA) and stearic acid (SA) by 73 and 64%, respectively, in contrast to furnace baking resulting in a decrease in the amount of PA and SA contaminants by 50 and 37%, respectively. A lower amount of FFA contaminants was extracted from glass tubes during comparative analysis of the four different sample tubes studied. A combination of a methanol-washed glass tube and chloroform extraction solvent was determined to be the optimal method for minimizing the error in FFA quantification. The optimized sample preparation protocol used for FFA quantification can lower the amount of foreign palmitic acid and stearic acid to the sub-nanomolar level in the analysis of FFAs in skimmed milk.Hyejin ParkWoo-Young SongHyeonjeon ChaTae-Young KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hyejin Park Woo-Young Song Hyeonjeon Cha Tae-Young Kim Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry
description	Abstract Accurate and precise determination of free fatty acid (FFA) contents is essential for quality control and assurance in food production. Herein, a mass spectrometric study was performed to develop a sample preparation protocol that can minimize exogenous FFA contamination during the quantification of FFA in food. The quantities of exogenous FFAs were measured using various combinations of seven pretreatment methods for a sample tube, three extraction methods, and four types of sample tubes. Methanol washing could effectively reduce exogenous palmitic acid (PA) and stearic acid (SA) by 73 and 64%, respectively, in contrast to furnace baking resulting in a decrease in the amount of PA and SA contaminants by 50 and 37%, respectively. A lower amount of FFA contaminants was extracted from glass tubes during comparative analysis of the four different sample tubes studied. A combination of a methanol-washed glass tube and chloroform extraction solvent was determined to be the optimal method for minimizing the error in FFA quantification. The optimized sample preparation protocol used for FFA quantification can lower the amount of foreign palmitic acid and stearic acid to the sub-nanomolar level in the analysis of FFAs in skimmed milk.
format	article
author	Hyejin Park Woo-Young Song Hyeonjeon Cha Tae-Young Kim
author_facet	Hyejin Park Woo-Young Song Hyeonjeon Cha Tae-Young Kim
author_sort	Hyejin Park
title	Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry
title_short	Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry
title_full	Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry
title_fullStr	Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry
title_full_unstemmed	Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry
title_sort	development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/085081ab168a4f82b7cda1ba79735a7c
work_keys_str_mv	AT hyejinpark developmentofanoptimizedsamplepreparationmethodforquantificationoffreefattyacidsinfoodusingliquidchromatographymassspectrometry AT wooyoungsong developmentofanoptimizedsamplepreparationmethodforquantificationoffreefattyacidsinfoodusingliquidchromatographymassspectrometry AT hyeonjeoncha developmentofanoptimizedsamplepreparationmethodforquantificationoffreefattyacidsinfoodusingliquidchromatographymassspectrometry AT taeyoungkim developmentofanoptimizedsamplepreparationmethodforquantificationoffreefattyacidsinfoodusingliquidchromatographymassspectrometry
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Development of an optimized sample preparation method for quantification of free fatty acids in food using liquid chromatography-mass spectrometry

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